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Strain Name:
C57BL/6J-MtgxR8831Btlr/Mmmh
Stock Number:
068659-MU
Citation ID:
RRID:MMRRC_068659-MU
Other Names:
R8831 (G1)
Major Collection:

Strain Information

Tyrp1
Name: tyrosinase-related protein 1
Synonyms: Tyrp, isa, TRP-1, TRP1, Oca3
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 22178
Homologene: 464
Atrn
Name: attractin
Synonyms: Mgca
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 11990
HGNC: HGNC:885
Homologene: 22542
Ncor1
Name: nuclear receptor co-repressor 1
Synonyms: N-CoR, Rxrip13, A230020K14Rik, 5730405M06Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20185
HGNC: HGNC:7672
Homologene: 38166
Nrcam
Name: neuronal cell adhesion molecule
Synonyms: Bravo, C030017F07Rik, C130076O07Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 319504
VEGA: 12
HGNC: HGNC:7994
Homologene: 21041
Mdm4
Name: transformed mouse 3T3 cell double minute 4
Synonyms: Mdmx, 4933417N07Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 17248
HGNC: HGNC:6974
Homologene: 1794
Phf3
Name: PHD finger protein 3
Synonyms: 2310061N19Rik, AU020177
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 213109
HGNC: HGNC:8921
Homologene: 9040
Usp48
Name: ubiquitin specific peptidase 48
Synonyms: D330022K21Rik, 2810449C13Rik, Usp31
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 170707
Homologene: 12988
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 30,821,266 bp
  • T to C, chromosome 1 at 34,444,364 bp
  • A to T, chromosome 1 at 34,472,190 bp
  • T to C, chromosome 1 at 133,003,863 bp
  • T to C, chromosome 1 at 144,001,759 bp
  • T to A, chromosome 1 at 162,574,383 bp
  • C to T, chromosome 1 at 164,256,874 bp
  • A to T, chromosome 2 at 24,349,493 bp
  • G to A, chromosome 2 at 36,479,782 bp
  • C to A, chromosome 2 at 91,190,746 bp
  • T to A, chromosome 2 at 98,663,989 bp
  • T to A, chromosome 2 at 127,257,059 bp
  • T to C, chromosome 2 at 130,906,601 bp
  • G to A, chromosome 2 at 147,085,194 bp
  • C to A, chromosome 2 at 152,622,979 bp
  • A to T, chromosome 2 at 154,554,687 bp
  • G to C, chromosome 2 at 160,747,812 bp
  • T to A, chromosome 2 at 160,780,771 bp
  • G to A, chromosome 3 at 68,962,250 bp
  • T to C, chromosome 3 at 83,285,363 bp
  • A to T, chromosome 3 at 98,446,731 bp
  • C to T, chromosome 4 at 59,379,594 bp
  • G to A, chromosome 4 at 80,835,162 bp
  • A to G, chromosome 4 at 104,790,677 bp
  • A to T, chromosome 4 at 120,567,310 bp
  • A to T, chromosome 4 at 137,613,769 bp
  • G to A, chromosome 4 at 149,646,323 bp
  • A to G, chromosome 5 at 34,459,030 bp
  • G to A, chromosome 5 at 107,720,272 bp
  • A to T, chromosome 5 at 109,676,308 bp
  • G to C, chromosome 5 at 136,697,971 bp
  • G to A, chromosome 5 at 137,567,043 bp
  • T to C, chromosome 6 at 57,233,520 bp
  • A to G, chromosome 6 at 88,084,793 bp
  • G to T, chromosome 7 at 27,518,113 bp
  • A to G, chromosome 7 at 49,828,981 bp
  • G to T, chromosome 7 at 85,409,810 bp
  • T to C, chromosome 7 at 100,634,724 bp
  • T to A, chromosome 7 at 103,172,996 bp
  • A to T, chromosome 8 at 12,279,688 bp
  • A to T, chromosome 8 at 36,146,737 bp
  • C to T, chromosome 8 at 48,276,382 bp
  • G to A, chromosome 8 at 95,062,217 bp
  • A to T, chromosome 8 at 106,210,557 bp
  • A to G, chromosome 8 at 119,749,294 bp
  • A to G, chromosome 9 at 21,580,367 bp
  • T to A, chromosome 9 at 107,300,472 bp
  • T to C, chromosome 9 at 108,564,758 bp
  • A to G, chromosome 9 at 110,088,251 bp
  • A to G, chromosome 10 at 18,652,743 bp
  • A to G, chromosome 10 at 84,613,658 bp
  • G to A, chromosome 10 at 130,391,099 bp
  • G to A, chromosome 11 at 3,913,087 bp
  • T to A, chromosome 11 at 7,161,362 bp
  • G to A, chromosome 11 at 40,721,672 bp
  • A to T, chromosome 11 at 60,725,248 bp
  • A to G, chromosome 11 at 61,216,316 bp
  • A to T, chromosome 11 at 62,369,408 bp
  • A to G, chromosome 11 at 63,964,480 bp
  • A to T, chromosome 11 at 81,967,900 bp
  • A to C, chromosome 11 at 94,350,961 bp
  • T to A, chromosome 11 at 100,703,980 bp
  • T to C, chromosome 11 at 107,176,836 bp
  • A to T, chromosome 11 at 115,001,018 bp
  • A to T, chromosome 12 at 25,036,455 bp
  • T to A, chromosome 12 at 44,544,897 bp
  • C to A, chromosome 13 at 47,064,141 bp
  • G to T, chromosome 13 at 112,504,380 bp
  • A to G, chromosome 13 at 118,789,135 bp
  • T to A, chromosome 14 at 32,560,827 bp
  • T to C, chromosome 14 at 54,894,457 bp
  • A to T, chromosome 14 at 70,967,063 bp
  • T to C, chromosome 15 at 8,182,136 bp
  • A to G, chromosome 15 at 102,486,018 bp
  • C to T, chromosome 16 at 20,550,464 bp
  • A to G, chromosome 17 at 24,157,738 bp
  • C to T, chromosome 17 at 25,834,883 bp
  • G to A, chromosome 17 at 28,080,219 bp
  • A to T, chromosome 17 at 35,514,897 bp
  • A to G, chromosome 17 at 35,888,108 bp
  • A to T, chromosome 17 at 71,752,310 bp
  • G to T, chromosome 17 at 84,668,995 bp
  • A to G, chromosome 17 at 91,089,003 bp
  • T to A, chromosome 18 at 12,200,820 bp
  • T to C, chromosome 18 at 20,320,308 bp
  • A to G, chromosome 18 at 37,437,333 bp
  • A to G, chromosome 19 at 4,269,025 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8831 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068659-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.