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Strain Name:
C57BL/6J-MtgxR8839Btlr/Mmmh
Stock Number:
068667-MU
Citation ID:
RRID:MMRRC_068667-MU
Other Names:
R8839 (G1)
Major Collection:

Strain Information

Sema3b
Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B
Synonyms: SemA, semaV, sema5, SemA, LUCA-1, Semaa
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20347
Homologene: 20972
Gpr37
Name: G protein-coupled receptor 37
Synonyms: Pael-R, parkin-associated endothelin B-like receptor
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 14763
HGNC: HGNC:4494
Homologene: 3875
Ptch1
Name: patched 1
Synonyms: Ptc, Patched 1, Ptc1, A230106A15Rik, wig
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 19206
HGNC: HGNC:9585
Homologene: 223
Inpp5d
Name: inositol polyphosphate-5-phosphatase D
Synonyms: SHIP, Src homology 2 domain-containing inositol-5-phosphatase, s-SHIP, SHIP-1, SHIP1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16331
HGNC: HGNC:6079
Homologene: 4046
Inpp5a
Name: inositol polyphosphate-5-phosphatase A
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 212111
HGNC: HGNC:6076
Homologene: 4045
Ints6
Name: integrator complex subunit 6
Synonyms: Notch2l, 2900075H24Rik, DICE1, Ddx26
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 18130
VEGA: 14
Homologene: 8121
Smug1
Name: single-strand selective monofunctional uracil DNA glycosylase
Synonyms: 1200013B09Rik, A930006H09Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 71726
VEGA: 15
Homologene: 8629
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 40,774,394 bp
  • T to A, chromosome 1 at 87,691,711 bp
  • T to C, chromosome 2 at 76,667,237 bp
  • C to T, chromosome 2 at 132,101,472 bp
  • A to G, chromosome 3 at 5,401,855 bp
  • A to G, chromosome 3 at 34,046,682 bp
  • A to G, chromosome 4 at 58,286,151 bp
  • A to G, chromosome 4 at 118,754,214 bp
  • A to C, chromosome 4 at 128,442,888 bp
  • A to G, chromosome 4 at 129,182,207 bp
  • T to A, chromosome 4 at 156,222,589 bp
  • T to C, chromosome 5 at 30,907,591 bp
  • A to G, chromosome 5 at 87,254,556 bp
  • A to T, chromosome 5 at 146,109,086 bp
  • T to G, chromosome 6 at 25,669,370 bp
  • T to C, chromosome 6 at 48,931,040 bp
  • G to A, chromosome 6 at 113,519,970 bp
  • A to T, chromosome 6 at 123,396,556 bp
  • A to T, chromosome 6 at 148,873,027 bp
  • T to G, chromosome 7 at 26,200,760 bp
  • A to T, chromosome 7 at 84,946,281 bp
  • A to G, chromosome 7 at 103,740,814 bp
  • A to T, chromosome 7 at 110,042,016 bp
  • A to G, chromosome 7 at 133,653,915 bp
  • T to C, chromosome 7 at 139,389,433 bp
  • G to A, chromosome 9 at 22,356,172 bp
  • T to C, chromosome 9 at 27,094,434 bp
  • C to T, chromosome 9 at 107,601,353 bp
  • T to C, chromosome 10 at 75,656,816 bp
  • A to C, chromosome 10 at 81,348,915 bp
  • T to C, chromosome 11 at 60,718,607 bp
  • T to C, chromosome 11 at 72,730,566 bp
  • T to C, chromosome 11 at 105,998,368 bp
  • G to A, chromosome 11 at 119,156,648 bp
  • T to C, chromosome 12 at 50,342,833 bp
  • T to C, chromosome 12 at 58,990,995 bp
  • A to G, chromosome 12 at 104,237,779 bp
  • G to A, chromosome 12 at 114,140,765 bp
  • A to T, chromosome 13 at 63,541,224 bp
  • A to T, chromosome 13 at 77,999,662 bp
  • A to T, chromosome 13 at 119,357,637 bp
  • A to T, chromosome 14 at 50,710,500 bp
  • A to G, chromosome 14 at 62,693,673 bp
  • T to A, chromosome 15 at 73,058,238 bp
  • A to T, chromosome 15 at 89,103,478 bp
  • T to C, chromosome 15 at 103,155,820 bp
  • C to A, chromosome 16 at 87,418,502 bp
  • A to G, chromosome 16 at 89,884,939 bp
  • A to G, chromosome 17 at 33,950,893 bp
  • T to C, chromosome 17 at 58,950,242 bp
  • T to A, chromosome 17 at 85,988,421 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8839 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068667-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.