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Strain Name:
C57BL/6J-MtgxR8864Btlr/Mmmh
Stock Number:
068680-MU
Citation ID:
RRID:MMRRC_068680-MU
Other Names:
R8864 (G1)
Major Collection:

Strain Information

Tln2
Name: talin 2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 70549
VEGA: 9
Homologene: 56692
Setd5
Name: SET domain containing 5
Synonyms: 2900045N06Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 72895
Homologene: 12485
Nfia
Name: nuclear factor I/A
Synonyms: 1110047K16Rik, NF1-A, 9430022M17Rik, NF1A
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18027
HGNC: HGNC:7784
Homologene: 4086
Ggnbp2
Name: gametogenetin binding protein 2
Synonyms: DIF-3, Zfp403, D330017P12Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217039
Homologene: 11750
Rangap1
Name: RAN GTPase activating protein 1
Synonyms: Fug1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 19387
VEGA: 15
HGNC: HGNC:9854
Homologene: 55700
Wapl
Name: WAPL cohesin release factor
Synonyms: A530089A20Rik, Wapal
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 218914
Homologene: 41002
Eif3b
Name: eukaryotic translation initiation factor 3, subunit B
Synonyms: EIF3-P116, D5Wsu45e, Eif3s9
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 27979
HGNC: HGNC:3280
Homologene: 2780
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 127,909,893 bp
  • T to A, chromosome 1 at 169,690,421 bp
  • C to T, chromosome 2 at 41,112,706 bp
  • C to T, chromosome 2 at 90,854,930 bp
  • T to A, chromosome 3 at 155,187,938 bp
  • T to C, chromosome 4 at 43,174,724 bp
  • C to T, chromosome 4 at 63,993,059 bp
  • T to C, chromosome 4 at 96,121,513 bp
  • T to C, chromosome 4 at 98,063,145 bp
  • T to A, chromosome 4 at 100,994,829 bp
  • T to C, chromosome 5 at 89,707,122 bp
  • T to C, chromosome 5 at 140,426,532 bp
  • A to G, chromosome 6 at 83,035,758 bp
  • T to A, chromosome 6 at 87,054,623 bp
  • G to A, chromosome 6 at 113,111,508 bp
  • A to G, chromosome 6 at 131,685,669 bp
  • A to G, chromosome 6 at 148,181,895 bp
  • T to C, chromosome 7 at 29,934,810 bp
  • A to T, chromosome 8 at 47,547,841 bp
  • T to A, chromosome 8 at 85,009,313 bp
  • T to C, chromosome 9 at 13,244,321 bp
  • G to T, chromosome 9 at 67,330,552 bp
  • T to C, chromosome 10 at 5,420,473 bp
  • T to A, chromosome 10 at 56,087,559 bp
  • A to C, chromosome 11 at 36,027,195 bp
  • G to A, chromosome 11 at 84,840,076 bp
  • T to C, chromosome 11 at 102,436,385 bp
  • C to T, chromosome 12 at 114,665,999 bp
  • C to T, chromosome 13 at 36,877,779 bp
  • T to C, chromosome 13 at 49,787,512 bp
  • T to C, chromosome 13 at 100,426,320 bp
  • G to T, chromosome 14 at 29,333,778 bp
  • T to A, chromosome 14 at 34,692,202 bp
  • T to C, chromosome 15 at 9,599,747 bp
  • T to C, chromosome 15 at 36,265,306 bp
  • C to G, chromosome 15 at 81,726,069 bp
  • A to T, chromosome 17 at 24,899,518 bp
  • T to C, chromosome 17 at 29,931,321 bp
  • T to C, chromosome 17 at 30,762,642 bp
  • T to A, chromosome 18 at 58,890,425 bp
  • C to A, chromosome 19 at 4,988,528 bp
  • T to C, chromosome 19 at 6,211,620 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8864 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068680-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.