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Strain Name:
C57BL/6J-MtgxR8933Btlr/Mmmh
Stock Number:
068709-MU
Citation ID:
RRID:MMRRC_068709-MU
Other Names:
R8933 (G1)
Major Collection:

Strain Information

Hmox1
Name: heme oxygenase 1
Synonyms: Hsp32, HO-1, heme oxygenase 1, D8Wsu38e, Hmox, HO1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 15368
HGNC: HGNC:5013
Homologene: 31075
Drd1
Name: dopamine receptor D1
Synonyms: D1 receptor, Gpcr15, Drd-1, C030036C15Rik, Drd1a
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 13488
VEGA: 13
HGNC: HGNC:3020
Homologene: 30992
Nap1l1
Name: nucleosome assembly protein 1-like 1
Synonyms: D10Ertd68e
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 53605
VEGA: 10
HGNC: HGNC:7637
Homologene: 129218
Tanc1
Name: tetratricopeptide repeat, ankyrin repeat and coiled-coil containing 1
Synonyms: 1200003E16Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 66860
Homologene: 18946
Zfp113
Name: zinc finger protein 113
Synonyms: 4732456B05Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 56314
Homologene: 50027
Wnk1
Name: WNK lysine deficient protein kinase 1
Synonyms: 6430573H23Rik, Prkwnk1, EG406236, Hsn2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232341
Homologene: 14253
Kdm3a
Name: lysine (K)-specific demethylase 3A
Synonyms: 1700105C21Rik, Tsga, C230043E16Rik, Jmjd1, Jmjd1a
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 104263
Homologene: 10196
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 45,421,963 bp
  • T to C, chromosome 1 at 106,753,219 bp
  • A to T, chromosome 1 at 155,863,415 bp
  • A to T, chromosome 1 at 181,139,073 bp
  • A to T, chromosome 2 at 35,085,702 bp
  • T to C, chromosome 2 at 38,760,388 bp
  • T to G, chromosome 2 at 59,785,456 bp
  • C to T, chromosome 2 at 60,627,903 bp
  • T to C, chromosome 2 at 76,378,724 bp
  • A to G, chromosome 2 at 97,629,481 bp
  • A to G, chromosome 2 at 120,338,585 bp
  • T to A, chromosome 2 at 146,942,117 bp
  • T to C, chromosome 3 at 88,747,704 bp
  • T to A, chromosome 3 at 90,279,216 bp
  • T to C, chromosome 3 at 101,823,458 bp
  • T to C, chromosome 3 at 104,840,893 bp
  • T to A, chromosome 3 at 106,129,017 bp
  • T to A, chromosome 3 at 117,323,041 bp
  • T to A, chromosome 3 at 122,128,137 bp
  • T to A, chromosome 3 at 125,611,506 bp
  • T to C, chromosome 3 at 141,856,608 bp
  • A to T, chromosome 4 at 25,262,258 bp
  • A to T, chromosome 4 at 25,619,861 bp
  • A to T, chromosome 4 at 33,146,972 bp
  • T to C, chromosome 5 at 90,258,466 bp
  • A to G, chromosome 5 at 103,579,805 bp
  • T to A, chromosome 5 at 108,703,391 bp
  • T to A, chromosome 5 at 122,113,202 bp
  • C to A, chromosome 5 at 137,290,187 bp
  • T to C, chromosome 5 at 138,144,830 bp
  • A to T, chromosome 6 at 34,927,689 bp
  • A to G, chromosome 6 at 42,818,016 bp
  • C to T, chromosome 6 at 66,732,247 bp
  • A to G, chromosome 6 at 71,600,108 bp
  • A to G, chromosome 6 at 120,036,998 bp
  • C to T, chromosome 7 at 3,296,917 bp
  • A to C, chromosome 7 at 7,002,797 bp
  • G to A, chromosome 7 at 13,339,702 bp
  • T to A, chromosome 7 at 25,023,318 bp
  • A to G, chromosome 7 at 49,461,957 bp
  • A to G, chromosome 7 at 56,984,958 bp
  • G to T, chromosome 7 at 74,284,500 bp
  • A to T, chromosome 7 at 79,117,630 bp
  • A to T, chromosome 7 at 102,604,430 bp
  • A to T, chromosome 7 at 104,617,666 bp
  • A to G, chromosome 7 at 104,861,100 bp
  • A to G, chromosome 7 at 105,069,392 bp
  • A to G, chromosome 7 at 112,898,611 bp
  • G to A, chromosome 7 at 127,552,394 bp
  • A to G, chromosome 7 at 141,789,756 bp
  • T to A, chromosome 8 at 22,111,210 bp
  • A to T, chromosome 8 at 22,120,843 bp
  • C to T, chromosome 8 at 48,279,060 bp
  • T to C, chromosome 8 at 75,097,016 bp
  • T to C, chromosome 9 at 44,822,505 bp
  • A to G, chromosome 9 at 103,448,649 bp
  • A to T, chromosome 10 at 17,806,798 bp
  • G to A, chromosome 10 at 20,357,528 bp
  • C to T, chromosome 10 at 21,367,685 bp
  • T to C, chromosome 10 at 77,565,188 bp
  • C to A, chromosome 10 at 91,083,230 bp
  • T to C, chromosome 10 at 111,492,849 bp
  • A to T, chromosome 10 at 129,322,390 bp
  • T to C, chromosome 11 at 5,524,741 bp
  • T to C, chromosome 11 at 7,198,333 bp
  • T to A, chromosome 11 at 65,855,252 bp
  • A to C, chromosome 11 at 95,021,201 bp
  • C to T, chromosome 11 at 96,107,426 bp
  • T to C, chromosome 11 at 98,005,752 bp
  • T to C, chromosome 11 at 99,321,238 bp
  • G to T, chromosome 11 at 106,250,834 bp
  • A to G, chromosome 13 at 14,054,343 bp
  • T to A, chromosome 13 at 22,524,521 bp
  • A to G, chromosome 13 at 54,053,271 bp
  • C to T, chromosome 13 at 59,769,683 bp
  • C to T, chromosome 13 at 62,777,040 bp
  • T to A, chromosome 14 at 118,906,914 bp
  • C to T, chromosome 14 at 121,486,679 bp
  • T to A, chromosome 15 at 98,909,269 bp
  • A to G, chromosome 16 at 18,400,095 bp
  • A to T, chromosome 17 at 12,701,244 bp
  • G to A, chromosome 17 at 12,704,637 bp
  • A to G, chromosome 17 at 18,583,979 bp
  • A to G, chromosome 17 at 34,804,311 bp
  • C to A, chromosome 17 at 37,225,455 bp
  • A to G, chromosome 19 at 34,069,480 bp
  • T to C, chromosome 19 at 34,594,013 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8933 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068709-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.