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Strain Name:
C57BL/6J-MtgxR8825Btlr/Mmmh
Stock Number:
068728-MU
Citation ID:
RRID:MMRRC_068728-MU
Other Names:
R8825 (G1)
Major Collection:

Strain Information

Gsta4
Name: glutathione S-transferase, alpha 4
Synonyms: GST 5.7, mGsta4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 14860
VEGA: 9
Homologene: 135605
Mycbp2
Name: MYC binding protein 2, E3 ubiquitin protein ligase
Synonyms: Pam, C130061D10Rik, Phr1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 105689
Homologene: 9005
Mark2
Name: MAP/microtubule affinity regulating kinase 2
Synonyms: Par-1, Emk
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 13728
HGNC: HGNC:3332
Homologene: 69013
Arfgap2
Name: ARF GTPase activating protein 2
Synonyms: 2310032E02Rik, Zfp289
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 77038
Homologene: 8735
Birc6
Name: baculoviral IAP repeat-containing 6
Synonyms: apollon, Bruce, A430032G04Rik, D630005A10Rik, A430040A19Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 12211
Homologene: 7248
Cdkal1
Name: CDK5 regulatory subunit associated protein 1-like 1
Synonyms: 6620401C13Rik, 1190005B03Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 68916
Homologene: 9830
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 9,960,999 bp
  • T to A, chromosome 1 at 186,628,939 bp
  • A to G, chromosome 2 at 10,190,420 bp
  • A to G, chromosome 2 at 26,423,574 bp
  • T to A, chromosome 2 at 91,273,561 bp
  • A to G, chromosome 2 at 158,267,750 bp
  • T to C, chromosome 2 at 168,951,632 bp
  • T to A, chromosome 3 at 53,863,672 bp
  • T to A, chromosome 3 at 90,259,156 bp
  • A to T, chromosome 3 at 152,276,823 bp
  • T to A, chromosome 4 at 43,237,683 bp
  • T to C, chromosome 4 at 148,140,820 bp
  • G to A, chromosome 4 at 148,568,702 bp
  • T to A, chromosome 4 at 155,885,130 bp
  • T to C, chromosome 5 at 34,825,960 bp
  • T to C, chromosome 5 at 98,942,184 bp
  • T to C, chromosome 5 at 109,339,153 bp
  • T to G, chromosome 5 at 109,678,880 bp
  • C to T, chromosome 5 at 135,422,122 bp
  • G to C, chromosome 6 at 23,654,379 bp
  • A to T, chromosome 7 at 15,627,301 bp
  • A to G, chromosome 7 at 24,929,257 bp
  • T to A, chromosome 7 at 56,050,878 bp
  • A to T, chromosome 7 at 86,803,647 bp
  • T to A, chromosome 7 at 104,411,322 bp
  • T to A, chromosome 7 at 105,693,967 bp
  • A to T, chromosome 7 at 106,845,429 bp
  • T to C, chromosome 7 at 143,083,016 bp
  • T to A, chromosome 8 at 3,386,951 bp
  • A to T, chromosome 8 at 60,929,488 bp
  • A to C, chromosome 8 at 66,513,667 bp
  • C to T, chromosome 8 at 104,967,322 bp
  • T to C, chromosome 9 at 39,750,698 bp
  • A to G, chromosome 9 at 57,241,471 bp
  • T to G, chromosome 9 at 78,208,839 bp
  • C to T, chromosome 9 at 108,485,261 bp
  • A to G, chromosome 10 at 81,325,658 bp
  • A to T, chromosome 10 at 123,013,871 bp
  • T to A, chromosome 10 at 128,291,364 bp
  • T to C, chromosome 11 at 29,823,696 bp
  • T to C, chromosome 11 at 46,162,046 bp
  • A to G, chromosome 11 at 115,797,375 bp
  • T to A, chromosome 12 at 119,445,852 bp
  • T to A, chromosome 13 at 13,005,071 bp
  • C to T, chromosome 13 at 29,354,794 bp
  • T to G, chromosome 13 at 69,813,927 bp
  • C to A, chromosome 13 at 73,665,632 bp
  • T to G, chromosome 13 at 108,384,782 bp
  • G to T, chromosome 14 at 7,887,566 bp
  • T to A, chromosome 14 at 47,380,100 bp
  • C to A, chromosome 14 at 103,229,435 bp
  • T to C, chromosome 15 at 4,731,688 bp
  • T to G, chromosome 15 at 32,689,352 bp
  • T to C, chromosome 15 at 83,552,611 bp
  • T to A, chromosome 16 at 22,562,769 bp
  • A to G, chromosome 16 at 36,919,447 bp
  • G to A, chromosome 16 at 45,394,794 bp
  • T to A, chromosome 17 at 74,613,505 bp
  • A to T, chromosome 18 at 3,268,061 bp
  • A to G, chromosome 18 at 53,409,150 bp
  • T to C, chromosome 18 at 74,759,098 bp
  • T to C, chromosome 19 at 7,341,206 bp
  • A to G, chromosome 19 at 24,949,237 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8825 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068728-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.