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Strain Name:
C57BL/6J-MtgxR8826Btlr/Mmmh
Stock Number:
068729-MU
Citation ID:
RRID:MMRRC_068729-MU
Other Names:
R8826 (G1)
Major Collection:

Strain Information

Fgg
Name: fibrinogen gamma chain
Synonyms: gamma-fibrinogen, 3010002H13Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 99571
HGNC: HGNC:3694
Homologene: 429
Ank2
Name: ankyrin 2, brain
Synonyms: ankyrin B, Ank-2, Ankyrin-2, Ankyrin-B, Gm4392
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 109676
HGNC: HGNC:493
Olig1
Name: oligodendrocyte transcription factor 1
Synonyms: Olg-1, Bhlhb6, bHLHe21
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 50914
Homologene: 9667
Cd86
Name: CD86 antigen
Synonyms: B7-2, MB7-2, B70, Ly-58, Cd28l2, B7.2, Ly58
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 12524
HGNC: HGNC:1705
Homologene: 10443
Ess2
Name: ess-2 splicing factor
Synonyms: ES2, Dgsi, D16H22S1269E, Es2el, Dgcr14
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 27886
VEGA: 16
Homologene: 11184
Upf1
Name: UPF1 RNA helicase and ATPase
Synonyms: PNORF-1, B430202H16Rik, Rent1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 19704
HGNC: HGNC:9962
Homologene: 2185
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 75,142,881 bp
  • A to G, chromosome 1 at 105,554,102 bp
  • A to G, chromosome 1 at 134,765,992 bp
  • C to G, chromosome 1 at 167,356,550 bp
  • T to A, chromosome 2 at 18,162,542 bp
  • T to A, chromosome 2 at 36,786,843 bp
  • A to G, chromosome 2 at 76,881,127 bp
  • G to T, chromosome 2 at 153,941,897 bp
  • A to T, chromosome 2 at 166,835,466 bp
  • A to G, chromosome 3 at 19,985,575 bp
  • T to C, chromosome 3 at 36,164,106 bp
  • C to A, chromosome 3 at 58,548,628 bp
  • A to G, chromosome 3 at 83,014,318 bp
  • C to T, chromosome 3 at 87,922,394 bp
  • A to T, chromosome 3 at 104,085,346 bp
  • A to T, chromosome 3 at 126,947,302 bp
  • G to A, chromosome 3 at 129,271,418 bp
  • A to C, chromosome 4 at 41,121,823 bp
  • A to T, chromosome 4 at 95,992,195 bp
  • A to G, chromosome 4 at 109,312,308 bp
  • A to G, chromosome 4 at 123,382,229 bp
  • G to A, chromosome 4 at 148,568,702 bp
  • T to C, chromosome 4 at 154,986,683 bp
  • C to T, chromosome 5 at 74,564,527 bp
  • A to G, chromosome 5 at 140,469,679 bp
  • G to C, chromosome 6 at 23,654,379 bp
  • C to T, chromosome 6 at 71,908,661 bp
  • T to A, chromosome 6 at 118,734,875 bp
  • T to C, chromosome 7 at 3,240,991 bp
  • A to G, chromosome 7 at 17,054,746 bp
  • A to T, chromosome 7 at 41,375,203 bp
  • A to T, chromosome 7 at 42,927,017 bp
  • A to G, chromosome 7 at 56,106,396 bp
  • A to T, chromosome 7 at 79,377,366 bp
  • A to T, chromosome 7 at 127,194,085 bp
  • A to G, chromosome 7 at 145,308,309 bp
  • T to A, chromosome 8 at 61,945,956 bp
  • T to C, chromosome 8 at 69,816,098 bp
  • T to C, chromosome 8 at 70,338,280 bp
  • T to C, chromosome 8 at 87,471,792 bp
  • T to A, chromosome 8 at 104,953,102 bp
  • T to C, chromosome 8 at 123,268,470 bp
  • T to C, chromosome 8 at 123,893,239 bp
  • A to G, chromosome 8 at 124,305,608 bp
  • T to A, chromosome 9 at 89,601,181 bp
  • A to T, chromosome 9 at 106,184,776 bp
  • T to C, chromosome 9 at 108,798,180 bp
  • A to G, chromosome 10 at 8,762,105 bp
  • A to T, chromosome 10 at 76,603,599 bp
  • A to G, chromosome 10 at 79,979,149 bp
  • G to T, chromosome 10 at 99,263,607 bp
  • G to A, chromosome 11 at 46,154,643 bp
  • G to A, chromosome 11 at 50,176,861 bp
  • A to T, chromosome 11 at 58,329,764 bp
  • A to T, chromosome 11 at 65,849,916 bp
  • G to T, chromosome 11 at 82,062,218 bp
  • A to T, chromosome 11 at 97,688,332 bp
  • C to T, chromosome 11 at 101,186,829 bp
  • T to C, chromosome 12 at 13,227,331 bp
  • T to A, chromosome 12 at 13,352,874 bp
  • G to T, chromosome 12 at 69,258,618 bp
  • A to G, chromosome 12 at 79,238,968 bp
  • A to G, chromosome 12 at 82,342,433 bp
  • A to T, chromosome 12 at 112,766,776 bp
  • T to G, chromosome 13 at 17,252,595 bp
  • C to A, chromosome 13 at 55,362,555 bp
  • A to G, chromosome 14 at 32,209,218 bp
  • G to A, chromosome 14 at 54,075,489 bp
  • T to A, chromosome 14 at 70,531,272 bp
  • A to G, chromosome 14 at 110,751,369 bp
  • G to T, chromosome 14 at 120,919,954 bp
  • A to G, chromosome 15 at 85,066,328 bp
  • A to T, chromosome 15 at 89,549,395 bp
  • A to G, chromosome 15 at 102,001,435 bp
  • C to G, chromosome 16 at 4,766,002 bp
  • T to G, chromosome 16 at 10,966,557 bp
  • A to T, chromosome 16 at 17,905,090 bp
  • C to A, chromosome 16 at 22,065,116 bp
  • G to T, chromosome 16 at 36,615,288 bp
  • G to A, chromosome 16 at 45,394,794 bp
  • A to G, chromosome 16 at 91,270,139 bp
  • C to T, chromosome 16 at 94,431,970 bp
  • A to G, chromosome 17 at 30,983,984 bp
  • A to T, chromosome 18 at 23,753,831 bp
  • A to T, chromosome 18 at 34,498,017 bp
  • A to T, chromosome 18 at 37,762,248 bp
  • A to T, chromosome 18 at 52,915,309 bp
  • A to G, chromosome 18 at 67,654,088 bp
  • C to T, chromosome 19 at 3,471,984 bp
  • T to C, chromosome 19 at 45,007,995 bp
  • T to C, chromosome 19 at 47,536,270 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8826 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068729-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.