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Strain Name:
C57BL/6J-MtgxR8855Btlr/Mmmh
Stock Number:
068736-MU
Citation ID:
RRID:MMRRC_068736-MU
Other Names:
R8855 (G1)
Major Collection:

Strain Information

Pkd1
Name: polycystin 1, transient receptor potential channel interacting
Synonyms: polycystin-1, PC1, PC-1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18763
VEGA: 17
HGNC: HGNC:9008
Homologene: 250
Hap1
Name: huntingtin-associated protein 1
Synonyms: HAP-1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 15114
HGNC: HGNC:4812
Homologene: 2935
Hcn4
Name: hyperpolarization-activated, cyclic nucleotide-gated K+ 4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 330953
VEGA: 9
Homologene: 3997
Numa1
Name: nuclear mitotic apparatus protein 1
Synonyms: 6720401E04Rik, NuMA
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 101706
HGNC: HGNC:8059
Homologene: 38150
Btaf1
Name: B-TFIID TATA-box binding protein associated factor 1
Synonyms: E430027O22Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 107182
Homologene: 31978
Zc3h7b
Name: zinc finger CCCH type containing 7B
Synonyms: Scrg3
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 20286
VEGA: 15
Homologene: 9735
Ahctf1
Name: AT hook containing transcription factor 1
Synonyms: 6230412P20Rik, Elys
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226747
Homologene: 9142
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to C, chromosome 1 at 75,185,223 bp
  • G to A, chromosome 1 at 179,786,776 bp
  • C to A, chromosome 1 at 189,653,233 bp
  • G to A, chromosome 2 at 26,374,533 bp
  • G to A, chromosome 2 at 26,439,840 bp
  • A to G, chromosome 2 at 82,980,177 bp
  • G to T, chromosome 2 at 91,669,399 bp
  • T to A, chromosome 2 at 140,183,920 bp
  • T to A, chromosome 2 at 169,886,505 bp
  • C to A, chromosome 2 at 181,013,891 bp
  • T to C, chromosome 3 at 9,699,469 bp
  • T to C, chromosome 3 at 10,340,751 bp
  • C to T, chromosome 3 at 60,007,090 bp
  • T to A, chromosome 3 at 116,570,141 bp
  • A to T, chromosome 4 at 32,601,573 bp
  • A to G, chromosome 4 at 62,415,998 bp
  • A to T, chromosome 4 at 70,300,650 bp
  • A to G, chromosome 4 at 123,715,862 bp
  • A to G, chromosome 4 at 134,232,183 bp
  • A to T, chromosome 4 at 141,634,347 bp
  • A to T, chromosome 4 at 143,994,335 bp
  • C to A, chromosome 5 at 31,087,981 bp
  • T to A, chromosome 5 at 31,138,513 bp
  • A to G, chromosome 5 at 96,769,606 bp
  • A to G, chromosome 5 at 146,948,911 bp
  • A to T, chromosome 5 at 147,570,872 bp
  • A to T, chromosome 5 at 147,681,650 bp
  • A to T, chromosome 6 at 129,903,570 bp
  • A to T, chromosome 6 at 134,468,822 bp
  • A to G, chromosome 6 at 135,023,393 bp
  • T to C, chromosome 7 at 28,370,101 bp
  • A to C, chromosome 7 at 38,100,621 bp
  • A to T, chromosome 7 at 45,644,979 bp
  • G to C, chromosome 7 at 101,990,628 bp
  • G to T, chromosome 7 at 102,588,738 bp
  • T to A, chromosome 7 at 103,309,961 bp
  • G to T, chromosome 7 at 109,481,981 bp
  • A to G, chromosome 7 at 118,206,899 bp
  • G to A, chromosome 7 at 141,068,575 bp
  • C to T, chromosome 8 at 4,243,594 bp
  • C to A, chromosome 9 at 14,555,573 bp
  • T to A, chromosome 9 at 20,875,644 bp
  • C to G, chromosome 9 at 37,804,413 bp
  • T to A, chromosome 9 at 58,858,104 bp
  • G to A, chromosome 9 at 64,912,390 bp
  • T to C, chromosome 9 at 78,337,598 bp
  • C to T, chromosome 9 at 106,886,396 bp
  • T to C, chromosome 9 at 111,312,221 bp
  • T to G, chromosome 10 at 21,995,827 bp
  • C to T, chromosome 10 at 23,921,078 bp
  • T to C, chromosome 10 at 33,528,404 bp
  • A to T, chromosome 11 at 5,704,642 bp
  • T to A, chromosome 11 at 67,211,421 bp
  • C to A, chromosome 11 at 100,356,038 bp
  • T to A, chromosome 11 at 105,970,598 bp
  • A to G, chromosome 11 at 118,988,365 bp
  • T to C, chromosome 11 at 120,494,969 bp
  • T to C, chromosome 12 at 110,635,899 bp
  • A to G, chromosome 12 at 110,868,414 bp
  • T to A, chromosome 12 at 118,192,372 bp
  • A to G, chromosome 13 at 24,986,907 bp
  • A to T, chromosome 13 at 33,899,326 bp
  • T to C, chromosome 13 at 62,841,895 bp
  • A to T, chromosome 13 at 75,769,674 bp
  • A to T, chromosome 14 at 52,372,739 bp
  • A to G, chromosome 15 at 43,866,835 bp
  • G to A, chromosome 15 at 81,772,480 bp
  • A to T, chromosome 15 at 89,011,966 bp
  • A to T, chromosome 15 at 98,856,356 bp
  • A to T, chromosome 16 at 10,644,867 bp
  • A to T, chromosome 16 at 22,939,571 bp
  • A to G, chromosome 16 at 38,184,538 bp
  • T to A, chromosome 16 at 75,558,593 bp
  • T to C, chromosome 17 at 24,573,077 bp
  • T to C, chromosome 17 at 35,015,644 bp
  • A to T, chromosome 18 at 12,697,946 bp
  • T to C, chromosome 19 at 25,411,338 bp
  • T to A, chromosome 19 at 36,958,501 bp
  • C to T, chromosome 19 at 45,011,833 bp
  • C to T, chromosome Y at 1,395,758 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8855 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068736-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.