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Strain Detail Sheet

Strain Name:
C57BL/6J-MtgxR8887Btlr/Mmmh
Stock Number:
068752-MU
Citation ID:
RRID:MMRRC_068752-MU
Other Names:
R8887 (G1)
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Vcan
Name: versican
Synonyms: PG-M, hdf, heart defect, 5430420N07Rik, Cspg2, DPEAAE
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 13003
HGNC: HGNC:2464
Homologene: 3228

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Gldc
Name: glycine decarboxylase
Synonyms: D19Wsu57e, D030049L12Rik, b2b2679Clo
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 104174
VEGA: 19
HGNC: HGNC:4313
Homologene: 141

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IMPC
Google
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Gene Ontology
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Mouse Phenome DB
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Monarch
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ClinGen
Ldb1
Name: LIM domain binding 1
Synonyms: CLIM2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 16825
HGNC: HGNC:6532
Homologene: 2891

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ClinGen
Dlc1
Name: deleted in liver cancer 1
Synonyms: p122-RhoGAP, STARD12, Arhgap7, A730069N07Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 50768
HGNC: HGNC:2897
Homologene: 4442

Additional Resources
IMPC
Google
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Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
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ClinGen
Ppp1r13b
Name: protein phosphatase 1, regulatory subunit 13B
Synonyms: ASPP1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 21981
VEGA: 12
Homologene: 9090

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Dip2c
Name: disco interacting protein 2 homolog C
Synonyms: 2900024P20Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 208440
Homologene: 40996

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Adam23
Name: a disintegrin and metallopeptidase domain 23
Synonyms: MDC3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 23792
HGNC: HGNC:202
Homologene: 2826

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 40,718,849 bp
  • T to A, chromosome 1 at 63,515,585 bp
  • T to C, chromosome 1 at 66,415,599 bp
  • A to G, chromosome 1 at 82,870,804 bp
  • T to C, chromosome 1 at 90,828,226 bp
  • T to C, chromosome 1 at 172,285,655 bp
  • T to A, chromosome 1 at 173,974,914 bp
  • C to A, chromosome 1 at 175,772,284 bp
  • C to A, chromosome 1 at 182,973,767 bp
  • A to G, chromosome 2 at 32,594,098 bp
  • T to G, chromosome 2 at 76,718,895 bp
  • A to T, chromosome 2 at 76,741,588 bp
  • A to G, chromosome 2 at 87,532,843 bp
  • A to G, chromosome 2 at 90,021,925 bp
  • A to C, chromosome 2 at 122,289,563 bp
  • C to A, chromosome 2 at 160,909,188 bp
  • G to T, chromosome 3 at 37,033,354 bp
  • A to G, chromosome 3 at 62,339,980 bp
  • T to A, chromosome 3 at 79,651,982 bp
  • T to C, chromosome 3 at 84,969,242 bp
  • A to G, chromosome 3 at 131,302,854 bp
  • T to A, chromosome 3 at 133,109,242 bp
  • C to A, chromosome 3 at 145,085,049 bp
  • A to T, chromosome 4 at 45,058,887 bp
  • A to G, chromosome 4 at 47,287,091 bp
  • A to C, chromosome 4 at 84,291,470 bp
  • C to G, chromosome 4 at 98,930,948 bp
  • T to C, chromosome 4 at 133,411,947 bp
  • A to T, chromosome 4 at 140,796,484 bp
  • A to G, chromosome 4 at 143,134,201 bp
  • A to G, chromosome 4 at 143,812,687 bp
  • A to T, chromosome 4 at 147,613,762 bp
  • G to A, chromosome 5 at 23,743,478 bp
  • A to T, chromosome 5 at 72,329,610 bp
  • A to G, chromosome 5 at 86,159,202 bp
  • T to A, chromosome 5 at 110,205,760 bp
  • A to G, chromosome 5 at 121,295,478 bp
  • A to G, chromosome 6 at 7,866,379 bp
  • A to G, chromosome 6 at 72,586,511 bp
  • A to T, chromosome 6 at 115,891,919 bp
  • C to A, chromosome 6 at 120,738,201 bp
  • A to T, chromosome 6 at 129,401,740 bp
  • A to G, chromosome 7 at 3,322,341 bp
  • T to C, chromosome 7 at 4,431,523 bp
  • A to G, chromosome 7 at 13,296,787 bp
  • A to C, chromosome 7 at 28,980,476 bp
  • T to A, chromosome 7 at 43,408,584 bp
  • G to A, chromosome 8 at 14,179,682 bp
  • A to T, chromosome 8 at 36,584,327 bp
  • T to A, chromosome 8 at 61,533,478 bp
  • A to G, chromosome 8 at 84,726,414 bp
  • T to G, chromosome 9 at 89,087,589 bp
  • T to C, chromosome 10 at 3,267,255 bp
  • C to T, chromosome 10 at 5,604,208 bp
  • C to T, chromosome 10 at 7,154,467 bp
  • A to G, chromosome 10 at 78,034,464 bp
  • A to T, chromosome 10 at 110,774,813 bp
  • T to C, chromosome 10 at 121,351,154 bp
  • A to T, chromosome 10 at 129,691,503 bp
  • G to A, chromosome 11 at 6,225,665 bp
  • T to C, chromosome 11 at 65,855,384 bp
  • T to C, chromosome 11 at 96,156,666 bp
  • T to A, chromosome 11 at 96,284,397 bp
  • T to C, chromosome 11 at 116,854,700 bp
  • A to G, chromosome 12 at 80,168,423 bp
  • A to T, chromosome 12 at 111,836,996 bp
  • G to A, chromosome 12 at 113,360,225 bp
  • A to G, chromosome 12 at 113,762,510 bp
  • C to A, chromosome 12 at 115,593,412 bp
  • T to A, chromosome 13 at 9,623,953 bp
  • T to A, chromosome 13 at 67,098,729 bp
  • T to A, chromosome 13 at 67,863,509 bp
  • A to T, chromosome 13 at 70,602,931 bp
  • T to C, chromosome 13 at 89,704,907 bp
  • C to T, chromosome 13 at 115,088,915 bp
  • T to A, chromosome 14 at 6,874,222 bp
  • A to G, chromosome 14 at 31,311,040 bp
  • G to A, chromosome 14 at 67,724,567 bp
  • A to T, chromosome 15 at 8,361,787 bp
  • T to C, chromosome 15 at 68,184,466 bp
  • A to G, chromosome 15 at 76,447,274 bp
  • C to T, chromosome 15 at 76,454,324 bp
  • T to A, chromosome 15 at 78,904,340 bp
  • T to C, chromosome 15 at 80,374,693 bp
  • G to T, chromosome 15 at 101,953,311 bp
  • A to C, chromosome 16 at 34,227,126 bp
  • T to C, chromosome 16 at 58,408,907 bp
  • A to T, chromosome 16 at 59,074,483 bp
  • C to T, chromosome 17 at 13,896,139 bp
  • A to G, chromosome 17 at 26,067,953 bp
  • G to A, chromosome 17 at 27,118,677 bp
  • A to T, chromosome 17 at 37,313,751 bp
  • T to C, chromosome 17 at 56,868,285 bp
  • T to G, chromosome 17 at 75,179,082 bp
  • T to C, chromosome 18 at 73,573,729 bp
  • A to T, chromosome 19 at 9,284,124 bp
  • A to G, chromosome 19 at 30,133,756 bp
  • T to C, chromosome 19 at 46,034,855 bp

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8887 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
068752-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.