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Strain Name:
C57BL/6J-MtgxR8900Btlr/Mmmh
Stock Number:
068757-MU
Citation ID:
RRID:MMRRC_068757-MU
Other Names:
R8900 (G1)
Major Collection:

Strain Information

Tiparp
Name: TCDD-inducible poly(ADP-ribose) polymerase
Synonyms: DDF1, PARP-7, PARP7
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 99929
Homologene: 9167
Ppp1r13b
Name: protein phosphatase 1, regulatory subunit 13B
Synonyms: ASPP1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 21981
VEGA: 12
Homologene: 9090
Cwf19l2
Name: CWF19 like cell cycle control factor 2
Synonyms: 3230401L03Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 244672
Homologene: 12366
Dock9
Name: dedicator of cytokinesis 9
Synonyms: B230309H04Rik, D14Wsu89e, Zizimin1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 105445
Homologene: 41026
Ncaph2
Name: non-SMC condensin II complex, subunit H2
Synonyms: 0610010J20Rik, 2610524G04Rik, D15Ertd785e, Kleisin beta
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 52683
Homologene: 12045
Hip1
Name: huntingtin interacting protein 1
Synonyms: 2610109B09Rik, A930014B11Rik, E130315I21Rik, HIP-1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 215114
HGNC: HGNC:4913
Homologene: 68463
Cebpa
Name: CCAAT/enhancer binding protein alpha
Synonyms: C/EBP alpha, Cebp, C/ebpalpha
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 12606
HGNC: HGNC:1833
Homologene: 3211
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 45,799,127 bp
  • T to C, chromosome 1 at 58,595,665 bp
  • T to G, chromosome 1 at 83,198,177 bp
  • T to A, chromosome 1 at 162,307,876 bp
  • A to T, chromosome 2 at 34,905,472 bp
  • C to A, chromosome 2 at 73,273,519 bp
  • T to A, chromosome 2 at 79,314,988 bp
  • T to A, chromosome 2 at 130,393,440 bp
  • A to T, chromosome 2 at 154,863,573 bp
  • T to C, chromosome 3 at 5,398,864 bp
  • T to A, chromosome 3 at 65,553,182 bp
  • T to A, chromosome 3 at 87,367,575 bp
  • T to C, chromosome 3 at 100,952,640 bp
  • C to A, chromosome 3 at 101,018,746 bp
  • G to T, chromosome 4 at 43,781,432 bp
  • G to A, chromosome 4 at 155,436,730 bp
  • T to C, chromosome 4 at 156,225,621 bp
  • T to C, chromosome 5 at 23,811,884 bp
  • A to G, chromosome 5 at 43,820,600 bp
  • T to C, chromosome 5 at 109,363,753 bp
  • A to T, chromosome 5 at 109,427,997 bp
  • A to T, chromosome 5 at 125,778,820 bp
  • T to C, chromosome 5 at 135,430,290 bp
  • T to A, chromosome 5 at 143,563,473 bp
  • T to C, chromosome 6 at 9,247,601 bp
  • T to C, chromosome 6 at 90,129,860 bp
  • C to T, chromosome 6 at 115,780,933 bp
  • A to G, chromosome 6 at 123,023,916 bp
  • C to T, chromosome 7 at 8,194,793 bp
  • A to G, chromosome 7 at 35,120,481 bp
  • A to T, chromosome 7 at 131,302,468 bp
  • G to A, chromosome 7 at 140,228,565 bp
  • A to G, chromosome 8 at 25,983,356 bp
  • A to T, chromosome 8 at 27,529,604 bp
  • A to T, chromosome 8 at 48,236,402 bp
  • A to C, chromosome 8 at 105,891,225 bp
  • T to A, chromosome 9 at 3,447,245 bp
  • T to A, chromosome 9 at 19,647,412 bp
  • C to A, chromosome 9 at 30,954,634 bp
  • A to T, chromosome 9 at 88,957,258 bp
  • A to C, chromosome 9 at 121,767,639 bp
  • C to G, chromosome 10 at 78,000,781 bp
  • A to G, chromosome 10 at 81,387,901 bp
  • T to C, chromosome 11 at 74,289,587 bp
  • T to C, chromosome 11 at 97,149,501 bp
  • T to C, chromosome 11 at 110,154,392 bp
  • C to T, chromosome 12 at 8,790,460 bp
  • G to T, chromosome 12 at 31,640,693 bp
  • T to A, chromosome 12 at 111,872,344 bp
  • G to A, chromosome 13 at 38,181,179 bp
  • G to A, chromosome 14 at 33,271,923 bp
  • T to G, chromosome 14 at 55,596,232 bp
  • T to C, chromosome 14 at 121,580,528 bp
  • A to T, chromosome 14 at 123,983,914 bp
  • G to A, chromosome 15 at 31,297,684 bp
  • T to A, chromosome 15 at 89,369,391 bp
  • C to T, chromosome 16 at 38,752,042 bp
  • T to C, chromosome 16 at 55,844,660 bp
  • A to G, chromosome 17 at 20,041,662 bp
  • A to G, chromosome 17 at 24,880,040 bp
  • T to G, chromosome 18 at 37,012,746 bp
  • T to G, chromosome 18 at 47,291,115 bp
  • T to C, chromosome 18 at 63,115,025 bp
  • T to C, chromosome 18 at 69,564,690 bp
  • A to C, chromosome 19 at 8,337,976 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8900 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068757-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.