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Strain Name:
C57BL/6J-MtgxR8934Btlr/Mmmh
Stock Number:
068777-MU
Citation ID:
RRID:MMRRC_068777-MU
Other Names:
R8934 (G1)
Major Collection:

Strain Information

Lamb3
Name: laminin, beta 3
Synonyms: nicein, 125kDa
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16780
HGNC: HGNC:6490
Homologene: 191
Lhx3
Name: LIM homeobox protein 3
Synonyms: P-LIM, mLim-3, Lim3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16871
HGNC: HGNC:6595
Homologene: 7814
Slamf6
Name: SLAM family member 6
Synonyms: SF2000, NTB-A, KAL1b, KAL1, Ly108
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 30925
Homologene: 49945
Abce1
Name: ATP-binding cassette, sub-family E member 1
Synonyms: RNS4l (Eye), Oabp, Rnaseli
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 24015
HGNC: HGNC:69
Homologene: 2205
Dusp16
Name: dual specificity phosphatase 16
Synonyms: MKP-7, MKP7, 3830417M17Rik, D6Ertd213e
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 70686
Homologene: 15604
Shroom3
Name: shroom family member 3
Synonyms: D5Ertd287e, Shrm, Shrm3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 27428
Homologene: 9263
Zfp609
Name: zinc finger protein 609
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 214812
Homologene: 72220
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 20,392,010 bp
  • T to A, chromosome 1 at 52,649,709 bp
  • T to C, chromosome 1 at 59,114,179 bp
  • A to G, chromosome 1 at 171,126,121 bp
  • C to T, chromosome 1 at 171,917,771 bp
  • T to C, chromosome 1 at 181,622,723 bp
  • T to A, chromosome 1 at 191,368,638 bp
  • T to C, chromosome 1 at 193,162,725 bp
  • T to C, chromosome 1 at 193,338,860 bp
  • C to A, chromosome 2 at 26,202,246 bp
  • G to A, chromosome 2 at 26,634,854 bp
  • T to C, chromosome 2 at 71,051,718 bp
  • T to A, chromosome 2 at 90,282,012 bp
  • C to T, chromosome 2 at 119,769,249 bp
  • T to C, chromosome 2 at 131,856,181 bp
  • A to T, chromosome 2 at 165,352,794 bp
  • A to G, chromosome 3 at 93,395,912 bp
  • T to G, chromosome 3 at 96,526,593 bp
  • A to G, chromosome 3 at 104,889,318 bp
  • C to T, chromosome 4 at 131,818,986 bp
  • C to T, chromosome 4 at 144,363,775 bp
  • T to C, chromosome 5 at 41,819,601 bp
  • T to A, chromosome 5 at 92,941,725 bp
  • T to C, chromosome 5 at 140,710,249 bp
  • A to T, chromosome 5 at 145,794,976 bp
  • A to T, chromosome 6 at 134,741,676 bp
  • A to T, chromosome 7 at 16,248,477 bp
  • G to A, chromosome 7 at 19,179,813 bp
  • A to G, chromosome 7 at 39,411,129 bp
  • T to A, chromosome 7 at 44,657,428 bp
  • T to A, chromosome 7 at 85,561,980 bp
  • T to C, chromosome 7 at 103,561,083 bp
  • T to C, chromosome 8 at 22,660,391 bp
  • A to G, chromosome 8 at 79,703,032 bp
  • A to T, chromosome 8 at 95,765,067 bp
  • T to A, chromosome 8 at 113,736,878 bp
  • G to A, chromosome 8 at 119,614,796 bp
  • A to C, chromosome 8 at 124,384,259 bp
  • T to C, chromosome 9 at 21,127,120 bp
  • C to T, chromosome 9 at 65,703,279 bp
  • T to C, chromosome 9 at 83,391,856 bp
  • T to C, chromosome 9 at 107,592,270 bp
  • T to A, chromosome 11 at 50,407,878 bp
  • A to G, chromosome 11 at 58,998,259 bp
  • A to G, chromosome 11 at 101,431,501 bp
  • G to T, chromosome 11 at 102,314,029 bp
  • T to C, chromosome 13 at 6,556,630 bp
  • T to C, chromosome 13 at 19,593,180 bp
  • G to A, chromosome 14 at 32,660,657 bp
  • G to A, chromosome 14 at 35,321,707 bp
  • A to G, chromosome 15 at 11,299,929 bp
  • T to C, chromosome 15 at 76,450,186 bp
  • T to C, chromosome 15 at 79,536,016 bp
  • T to C, chromosome 15 at 98,861,886 bp
  • T to C, chromosome 16 at 26,676,984 bp
  • C to T, chromosome 17 at 23,973,654 bp
  • G to A, chromosome 17 at 34,732,984 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8934 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068777-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.