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Strain Name:
C57BL/6J-MtgxR8935Btlr/Mmmh
Stock Number:
068778-MU
Citation ID:
RRID:MMRRC_068778-MU
Other Names:
R8935 (G1)
Major Collection:

Strain Information

Zfp143
Name: zinc finger protein 143
Synonyms: D7Ertd805e, pHZ-1, KRAB14, Zfp79, Zfp80-rs1, Staf
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20841
Homologene: 56444
Sema3e
Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E
Synonyms: Semah
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 20349
Homologene: 8247
Gldc
Name: glycine decarboxylase
Synonyms: D19Wsu57e, D030049L12Rik, b2b2679Clo
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 104174
VEGA: 19
HGNC: HGNC:4313
Homologene: 141
Gli2
Name: GLI-Kruppel family member GLI2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14633
HGNC: HGNC:4318
Homologene: 12725
Rbm6
Name: RNA binding motif protein 6
Synonyms: NY-LU-12, g16, Def-3
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 19654
HGNC: HGNC:9903
Homologene: 31336
Nfya
Name: nuclear transcription factor-Y alpha
Synonyms: Sez10, Cbf-b
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18044
HGNC: HGNC:7804
Homologene: 32114
Supt20
Name: SPT20 SAGA complex component
Synonyms: p38 interacting protein, p38IP, D3Ertd300e, Fam48a
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 56790
Homologene: 134155
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 60,371,314 bp
  • A to T, chromosome 1 at 65,165,219 bp
  • A to T, chromosome 1 at 75,422,606 bp
  • A to G, chromosome 1 at 87,289,497 bp
  • T to C, chromosome 1 at 118,836,392 bp
  • C to A, chromosome 1 at 134,040,848 bp
  • A to G, chromosome 1 at 166,642,220 bp
  • C to T, chromosome 2 at 26,943,929 bp
  • T to C, chromosome 2 at 52,251,768 bp
  • T to C, chromosome 2 at 76,549,456 bp
  • A to G, chromosome 2 at 87,608,077 bp
  • A to AC, chromosome 2 at 98,667,033 bp
  • A to T, chromosome 2 at 111,691,899 bp
  • A to T, chromosome 2 at 112,678,057 bp
  • A to G, chromosome 3 at 54,727,567 bp
  • A to G, chromosome 3 at 104,870,888 bp
  • T to C, chromosome 4 at 108,092,875 bp
  • T to A, chromosome 4 at 126,043,407 bp
  • A to T, chromosome 4 at 141,685,289 bp
  • C to T, chromosome 5 at 9,441,764 bp
  • A to T, chromosome 5 at 14,232,113 bp
  • A to C, chromosome 5 at 33,735,466 bp
  • C to T, chromosome 5 at 36,035,858 bp
  • T to G, chromosome 5 at 150,568,981 bp
  • T to C, chromosome 6 at 66,776,995 bp
  • T to C, chromosome 6 at 69,283,928 bp
  • T to C, chromosome 6 at 83,138,215 bp
  • A to G, chromosome 6 at 145,543,503 bp
  • T to A, chromosome 7 at 42,788,367 bp
  • T to C, chromosome 7 at 73,503,462 bp
  • T to C, chromosome 7 at 80,115,198 bp
  • C to T, chromosome 7 at 100,878,117 bp
  • T to C, chromosome 7 at 110,070,529 bp
  • C to T, chromosome 7 at 118,698,691 bp
  • T to C, chromosome 8 at 40,794,952 bp
  • T to C, chromosome 8 at 47,192,860 bp
  • A to G, chromosome 8 at 71,492,537 bp
  • G to T, chromosome 8 at 116,971,442 bp
  • T to C, chromosome 9 at 65,075,784 bp
  • C to T, chromosome 9 at 107,800,746 bp
  • T to A, chromosome 9 at 110,816,459 bp
  • C to T, chromosome 10 at 127,850,060 bp
  • A to G, chromosome 11 at 73,136,064 bp
  • C to T, chromosome 11 at 76,840,469 bp
  • T to A, chromosome 11 at 101,038,693 bp
  • T to C, chromosome 11 at 104,507,200 bp
  • C to T, chromosome 11 at 118,276,831 bp
  • T to C, chromosome 12 at 29,827,244 bp
  • C to T, chromosome 12 at 91,266,996 bp
  • C to T, chromosome 13 at 22,354,922 bp
  • A to G, chromosome 13 at 69,619,467 bp
  • G to A, chromosome 14 at 35,321,707 bp
  • T to C, chromosome 15 at 9,607,350 bp
  • T to C, chromosome 15 at 27,591,026 bp
  • G to A, chromosome 15 at 50,888,948 bp
  • C to G, chromosome 15 at 76,370,392 bp
  • T to C, chromosome 16 at 89,884,933 bp
  • A to G, chromosome 17 at 20,583,695 bp
  • A to T, chromosome 17 at 48,393,266 bp
  • C to T, chromosome 18 at 67,862,472 bp
  • T to A, chromosome 19 at 10,531,981 bp
  • C to T, chromosome 19 at 30,131,693 bp
  • G to T, chromosome 19 at 55,194,700 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8935 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068778-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.