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Strain Name:
C57BL/6J-MtgxR8954Btlr/Mmmh
Stock Number:
068790-MU
Citation ID:
RRID:MMRRC_068790-MU
Other Names:
R8954 (G1)
Major Collection:

Strain Information

Sbf2
Name: SET binding factor 2
Synonyms: SBF2, 4833411B01Rik, mMTMH1, B430219L04Rik, Mtmr13
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 319934
HGNC: HGNC:2135
Homologene: 41810
Gabra6
Name: gamma-aminobutyric acid type A receptor subunit alpha 6
Synonyms: Gabra-6, alpha6, GABA-ARalpha6
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14399
HGNC: HGNC:4080
Homologene: 20220
Dap3
Name: death associated protein 3
Synonyms: 4921514D13Rik, DAP-3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 65111
HGNC: HGNC:2673
Homologene: 3404
Arid5b
Name: AT-rich interaction domain 5B
Synonyms: 5430435G07Rik, Desrt, Mrf2beta, Mrf2alpha, Mrf2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 71371
VEGA: 10
Homologene: 45872
Mtss1
Name: MTSS I-BAR domain containing 1
Synonyms: MIM, D130001D01Rik, 2310003N14Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 211401
VEGA: 15
Homologene: 8841
Gapdhs
Name: glyceraldehyde-3-phosphate dehydrogenase, spermatogenic
Synonyms: Gapd-s, Gapds
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 14447
Homologene: 101265
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 24,706,040 bp
  • C to T, chromosome 1 at 69,996,845 bp
  • A to G, chromosome 1 at 74,474,984 bp
  • G to A, chromosome 1 at 87,148,627 bp
  • A to T, chromosome 1 at 134,834,462 bp
  • A to G, chromosome 2 at 15,551,367 bp
  • A to G, chromosome 2 at 25,280,478 bp
  • A to G, chromosome 2 at 25,779,253 bp
  • A to G, chromosome 2 at 25,894,326 bp
  • T to C, chromosome 2 at 34,678,098 bp
  • T to A, chromosome 2 at 77,924,593 bp
  • C to A, chromosome 2 at 86,455,548 bp
  • T to A, chromosome 2 at 93,857,954 bp
  • C to T, chromosome 2 at 119,353,355 bp
  • C to G, chromosome 2 at 121,323,220 bp
  • C to T, chromosome 2 at 144,823,966 bp
  • T to C, chromosome 3 at 69,575,340 bp
  • T to C, chromosome 3 at 87,089,866 bp
  • A to T, chromosome 3 at 88,928,263 bp
  • T to A, chromosome 4 at 3,834,617 bp
  • G to T, chromosome 4 at 42,871,753 bp
  • A to T, chromosome 4 at 45,284,702 bp
  • T to A, chromosome 4 at 115,328,738 bp
  • T to A, chromosome 4 at 124,804,856 bp
  • G to A, chromosome 4 at 155,436,730 bp
  • A to G, chromosome 5 at 102,892,270 bp
  • G to A, chromosome 5 at 108,629,652 bp
  • A to T, chromosome 5 at 122,344,230 bp
  • T to A, chromosome 5 at 136,373,349 bp
  • C to T, chromosome 6 at 3,374,577 bp
  • C to T, chromosome 6 at 138,142,969 bp
  • T to C, chromosome 7 at 7,551,656 bp
  • C to A, chromosome 7 at 16,237,808 bp
  • C to T, chromosome 7 at 23,633,100 bp
  • T to A, chromosome 7 at 27,703,962 bp
  • T to C, chromosome 7 at 28,895,158 bp
  • T to A, chromosome 7 at 30,254,029 bp
  • T to G, chromosome 7 at 30,574,215 bp
  • T to A, chromosome 7 at 30,733,166 bp
  • G to A, chromosome 7 at 64,208,341 bp
  • TCCCAGG to T, chromosome 7 at 80,098,331 bp
  • T to C, chromosome 7 at 100,160,242 bp
  • C to A, chromosome 7 at 101,579,903 bp
  • T to C, chromosome 7 at 102,640,675 bp
  • T to A, chromosome 7 at 104,688,693 bp
  • A to T, chromosome 7 at 105,694,779 bp
  • T to A, chromosome 7 at 110,438,911 bp
  • T to C, chromosome 7 at 118,206,992 bp
  • A to T, chromosome 7 at 118,794,578 bp
  • G to A, chromosome 7 at 121,145,518 bp
  • T to C, chromosome 7 at 142,328,563 bp
  • T to A, chromosome 8 at 25,673,378 bp
  • T to A, chromosome 8 at 94,558,146 bp
  • A to C, chromosome 8 at 109,531,801 bp
  • A to G, chromosome 8 at 111,187,895 bp
  • T to C, chromosome 9 at 16,376,568 bp
  • A to T, chromosome 9 at 20,137,368 bp
  • G to A, chromosome 9 at 21,739,532 bp
  • C to T, chromosome 9 at 54,473,872 bp
  • A to G, chromosome 9 at 62,769,263 bp
  • C to A, chromosome 9 at 96,177,509 bp
  • A to T, chromosome 9 at 110,392,500 bp
  • C to T, chromosome 10 at 5,604,208 bp
  • A to C, chromosome 10 at 28,789,713 bp
  • A to C, chromosome 10 at 68,101,980 bp
  • G to T, chromosome 10 at 80,057,962 bp
  • A to T, chromosome 10 at 93,043,393 bp
  • A to G, chromosome 10 at 111,272,192 bp
  • T to A, chromosome 11 at 29,205,669 bp
  • T to A, chromosome 11 at 42,315,132 bp
  • T to A, chromosome 11 at 58,954,959 bp
  • T to A, chromosome 11 at 67,252,980 bp
  • T to A, chromosome 11 at 72,750,491 bp
  • T to G, chromosome 11 at 105,018,699 bp
  • T to C, chromosome 11 at 116,859,336 bp
  • A to T, chromosome 12 at 5,137,542 bp
  • T to C, chromosome 12 at 81,420,372 bp
  • T to C, chromosome 13 at 70,610,578 bp
  • T to C, chromosome 13 at 97,929,633 bp
  • T to C, chromosome 13 at 98,777,477 bp
  • T to A, chromosome 13 at 99,434,227 bp
  • T to C, chromosome 14 at 34,555,344 bp
  • T to C, chromosome 15 at 58,955,137 bp
  • T to A, chromosome 15 at 89,549,228 bp
  • T to A, chromosome 16 at 32,256,655 bp
  • A to G, chromosome 16 at 44,262,027 bp
  • T to A, chromosome 16 at 59,148,956 bp
  • T to A, chromosome 17 at 18,325,990 bp
  • A to G, chromosome 17 at 24,891,519 bp
  • A to T, chromosome 17 at 71,448,757 bp
  • A to T, chromosome 17 at 90,590,187 bp
  • G to A, chromosome 18 at 22,517,750 bp
  • T to G, chromosome 18 at 37,708,487 bp
  • G to A, chromosome 18 at 43,477,634 bp
  • T to G, chromosome 19 at 5,948,424 bp
  • A to T, chromosome 19 at 29,718,726 bp
  • A to T, chromosome 19 at 39,536,753 bp
  • A to T, chromosome 19 at 46,888,922 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8954 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068790-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.