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Strain Name:
C57BL/6J-MtgxR8965Btlr/Mmmh
Stock Number:
068799-MU
Citation ID:
RRID:MMRRC_068799-MU
Other Names:
R8965 (G1)
Major Collection:

Strain Information

Zbtb25
Name: zinc finger and BTB domain containing 25
Synonyms: Zfp-50, Kup, 2810462M08Rik, Zfp50, 2900064P18Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 109929
Homologene: 5070
Rapgef2
Name: Rap guanine nucleotide exchange factor (GEF) 2
Synonyms: 5830453M24Rik, Pdzgef1, RA-GEF-1, CNRasGEF, nRapGEP
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 76089
Homologene: 35477
Psma5
Name: proteasome subunit alpha 5
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 26442
HGNC: HGNC:9534
Homologene: 2084
Wac
Name: WW domain containing adaptor with coiled-coil
Synonyms: Wwp4, 1110067P07Rik, A230035H12Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225131
Homologene: 41148
Cdk5rap2
Name: CDK5 regulatory subunit associated protein 2
Synonyms: 2900018K03Rik, an
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 214444
Homologene: 49533
Ptpn11
Name: protein tyrosine phosphatase, non-receptor type 11
Synonyms: PTP2C, PTP1D, SH-PTP2, SHP-2, Syp, Shp2, SH2 domain-containing protein tyrosine phosphatase-2, 2700084A17Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 19247
HGNC: HGNC:9644
Homologene: 2122
Patz1
Name: POZ (BTB) and AT hook containing zinc finger 1
Synonyms: MAZR, 8430401L15Rik, POZ-AT hook-zinc finger protein, Zfp278
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 56218
Homologene: 8636
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 172,280,045 bp
  • T to A, chromosome 2 at 31,011,785 bp
  • A to T, chromosome 2 at 87,252,557 bp
  • T to C, chromosome 2 at 132,252,849 bp
  • A to T, chromosome 3 at 58,624,317 bp
  • A to G, chromosome 3 at 79,092,544 bp
  • T to A, chromosome 3 at 108,265,194 bp
  • T to A, chromosome 3 at 116,128,773 bp
  • A to C, chromosome 4 at 70,266,805 bp
  • T to A, chromosome 4 at 111,822,812 bp
  • G to T, chromosome 4 at 136,938,586 bp
  • A to T, chromosome 4 at 155,772,983 bp
  • A to G, chromosome 5 at 114,035,210 bp
  • G to A, chromosome 5 at 121,163,166 bp
  • A to G, chromosome 6 at 64,320,006 bp
  • A to T, chromosome 6 at 68,303,128 bp
  • T to C, chromosome 7 at 107,903,107 bp
  • T to C, chromosome 8 at 94,391,841 bp
  • A to G, chromosome 8 at 105,945,350 bp
  • T to A, chromosome 8 at 112,753,014 bp
  • G to A, chromosome 8 at 125,759,114 bp
  • A to G, chromosome 9 at 49,438,418 bp
  • C to T, chromosome 9 at 108,294,509 bp
  • A to G, chromosome 10 at 20,963,862 bp
  • T to C, chromosome 11 at 3,307,815 bp
  • T to C, chromosome 11 at 21,148,488 bp
  • A to T, chromosome 11 at 67,364,606 bp
  • T to C, chromosome 11 at 73,009,225 bp
  • A to G, chromosome 11 at 98,892,671 bp
  • G to T, chromosome 11 at 106,005,349 bp
  • T to G, chromosome 11 at 118,024,666 bp
  • A to C, chromosome 12 at 76,349,803 bp
  • T to A, chromosome 13 at 19,243,010 bp
  • T to C, chromosome 13 at 21,574,217 bp
  • T to C, chromosome 13 at 23,791,023 bp
  • T to A, chromosome 13 at 77,612,604 bp
  • T to C, chromosome 15 at 82,558,985 bp
  • T to C, chromosome 16 at 17,509,432 bp
  • A to G, chromosome 16 at 20,596,326 bp
  • T to C, chromosome 16 at 25,972,384 bp
  • A to G, chromosome 17 at 17,838,456 bp
  • T to C, chromosome 17 at 21,396,893 bp
  • C to T, chromosome 17 at 24,445,530 bp
  • C to A, chromosome 18 at 7,905,936 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8965 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068799-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.