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Strain Name:
C57BL/6J-MtgxR8966Btlr/Mmmh
Stock Number:
068800-MU
Citation ID:
RRID:MMRRC_068800-MU
Other Names:
R8966 (G1)
Major Collection:

Strain Information

Pkd1
Name: polycystin 1, transient receptor potential channel interacting
Synonyms: polycystin-1, PC1, PC-1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18763
VEGA: 17
HGNC: HGNC:9008
Homologene: 250
Slc1a3
Name: solute carrier family 1 (glial high affinity glutamate transporter), member 3
Synonyms: MGluT1, Eaat1, B430115D02Rik, GLAST, Gmt1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 20512
Homologene: 20882
Srd5a3
Name: steroid 5 alpha-reductase 3
Synonyms: D730040M03Rik, 1110025P14Rik, Srd5a2l
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 57357
Homologene: 41385
Slc4a2
Name: solute carrier family 4 (anion exchanger), member 2
Synonyms: B3RP, Ae2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 20535
Homologene: 128699
Ndc80
Name: NDC80 kinetochore complex component
Synonyms: HEC1, 2610020P18Rik, Kntc2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 67052
VEGA: 17
Homologene: 38141
Usf1
Name: upstream transcription factor 1
Synonyms: upstream stimulatory factor, bHLHb11
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 22278
Homologene: 31426
Prcc
Name: papillary renal cell carcinoma (translocation-associated)
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 94315
HGNC: HGNC:9343
Homologene: 38120
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 16,080,838 bp
  • G to T, chromosome 1 at 53,066,482 bp
  • T to A, chromosome 1 at 87,087,488 bp
  • T to C, chromosome 1 at 107,000,435 bp
  • T to A, chromosome 1 at 133,738,579 bp
  • C to A, chromosome 1 at 133,901,134 bp
  • A to C, chromosome 1 at 143,646,145 bp
  • T to A, chromosome 1 at 171,417,533 bp
  • T to C, chromosome 1 at 191,619,605 bp
  • T to C, chromosome 2 at 25,200,739 bp
  • A to T, chromosome 2 at 76,692,662 bp
  • T to A, chromosome 2 at 87,022,535 bp
  • T to A, chromosome 2 at 165,353,038 bp
  • T to C, chromosome 3 at 14,537,299 bp
  • T to G, chromosome 3 at 28,532,895 bp
  • A to G, chromosome 3 at 34,078,744 bp
  • T to C, chromosome 3 at 87,884,925 bp
  • G to A, chromosome 3 at 122,961,332 bp
  • A to G, chromosome 3 at 144,839,111 bp
  • C to T, chromosome 4 at 32,672,837 bp
  • A to G, chromosome 4 at 33,152,411 bp
  • T to C, chromosome 4 at 134,202,401 bp
  • A to G, chromosome 5 at 24,391,202 bp
  • A to G, chromosome 5 at 24,430,094 bp
  • T to A, chromosome 5 at 76,153,590 bp
  • T to A, chromosome 5 at 112,450,237 bp
  • T to C, chromosome 5 at 138,291,404 bp
  • A to G, chromosome 5 at 144,803,352 bp
  • A to G, chromosome 6 at 23,970,021 bp
  • T to C, chromosome 6 at 49,446,200 bp
  • A to C, chromosome 6 at 86,987,252 bp
  • G to A, chromosome 6 at 123,742,120 bp
  • A to T, chromosome 6 at 148,999,976 bp
  • A to C, chromosome 7 at 11,066,323 bp
  • C to A, chromosome 7 at 12,991,163 bp
  • C to A, chromosome 7 at 81,344,912 bp
  • A to T, chromosome 7 at 103,470,317 bp
  • A to G, chromosome 7 at 104,068,932 bp
  • G to A, chromosome 7 at 118,561,005 bp
  • G to T, chromosome 7 at 119,950,658 bp
  • A to G, chromosome 7 at 140,858,466 bp
  • T to A, chromosome 8 at 16,200,045 bp
  • T to C, chromosome 8 at 26,978,274 bp
  • T to C, chromosome 8 at 109,948,387 bp
  • G to A, chromosome 9 at 21,914,764 bp
  • T to C, chromosome 9 at 22,099,420 bp
  • A to T, chromosome 9 at 110,725,161 bp
  • G to A, chromosome 10 at 95,997,039 bp
  • A to T, chromosome 10 at 129,501,082 bp
  • C to G, chromosome 11 at 9,628,588 bp
  • T to C, chromosome 11 at 95,890,985 bp
  • T to C, chromosome 11 at 103,209,317 bp
  • C to T, chromosome 11 at 110,071,419 bp
  • T to G, chromosome 12 at 17,369,506 bp
  • T to C, chromosome 12 at 76,099,423 bp
  • G to T, chromosome 12 at 87,773,503 bp
  • T to A, chromosome 13 at 3,642,093 bp
  • T to A, chromosome 13 at 32,978,052 bp
  • T to C, chromosome 13 at 63,347,471 bp
  • C to T, chromosome 14 at 55,512,603 bp
  • C to T, chromosome 15 at 8,650,848 bp
  • T to A, chromosome 15 at 11,001,036 bp
  • A to T, chromosome 15 at 85,817,811 bp
  • T to A, chromosome 16 at 37,611,170 bp
  • A to G, chromosome 17 at 24,575,777 bp
  • C to A, chromosome 17 at 71,508,578 bp
  • C to A, chromosome 17 at 80,398,450 bp
  • A to G, chromosome 18 at 37,422,984 bp
  • A to T, chromosome 19 at 13,313,832 bp
  • A to T, chromosome 19 at 36,734,336 bp
  • A to G, chromosome 19 at 46,065,679 bp
  • T to C, chromosome Y at 1,099,748 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8966 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068800-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.