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Strain Name:
C57BL/6J-MtgxR8971Btlr/Mmmh
Stock Number:
068805-MU
Citation ID:
RRID:MMRRC_068805-MU
Other Names:
R8971 (G1)
Major Collection:

Strain Information

Kif3a
Name: kinesin family member 3A
Synonyms: kinesin-II subunit, Kns3, Kif3, Kifl, N-4 kinesin
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16568
HGNC: HGNC:6319
Homologene: 38266
Cnnm2
Name: cyclin M2
Synonyms: Acdp2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 94219
VEGA: 19
HGNC: HGNC:103
Homologene: 9761
Gria2
Name: glutamate receptor, ionotropic, AMPA2 (alpha 2)
Synonyms: GluR-B, GluR2, Glur-2, Glur2, GluA2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14800
HGNC: HGNC:4572
Homologene: 20225
Nudt2
Name: nudix hydrolase 2
Synonyms: APAH1, 2310051L06Rik, nudix (nucleoside diphosphate linked moiety X)-type motif 2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 66401
HGNC: HGNC:8049
Homologene: 896
Spen
Name: spen family transcription repressor
Synonyms: Mint
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 56381
Homologene: 124461
Arfgef2
Name: ARF guanine nucleotide exchange factor 2
Synonyms: BIG2, E230011G24Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 99371
Homologene: 111880
Prkdc
Name: protein kinase, DNA activated, catalytic polypeptide
Synonyms: DNA-PKcs, slip, DNAPDcs, XRCC7, DNA-PK, DOXNPH, dxnph
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 19090
HGNC: HGNC:9413
Homologene: 5037
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 54,653,793 bp
  • G to A, chromosome 1 at 156,391,946 bp
  • C to T, chromosome 1 at 172,484,466 bp
  • C to A, chromosome 2 at 17,996,726 bp
  • T to C, chromosome 2 at 18,847,556 bp
  • T to C, chromosome 2 at 41,435,628 bp
  • A to T, chromosome 2 at 71,546,372 bp
  • T to C, chromosome 2 at 85,923,984 bp
  • T to A, chromosome 2 at 87,532,236 bp
  • T to A, chromosome 2 at 89,097,547 bp
  • T to C, chromosome 2 at 120,019,569 bp
  • A to G, chromosome 2 at 120,360,060 bp
  • T to A, chromosome 2 at 125,579,850 bp
  • G to A, chromosome 2 at 153,067,836 bp
  • A to T, chromosome 2 at 166,859,301 bp
  • T to C, chromosome 2 at 180,025,314 bp
  • T to C, chromosome 3 at 41,727,106 bp
  • C to T, chromosome 3 at 80,707,893 bp
  • A to C, chromosome 3 at 86,615,081 bp
  • T to C, chromosome 3 at 87,739,846 bp
  • T to A, chromosome 3 at 96,805,786 bp
  • C to T, chromosome 3 at 97,171,645 bp
  • A to T, chromosome 4 at 41,477,575 bp
  • C to A, chromosome 4 at 118,431,512 bp
  • T to A, chromosome 4 at 128,586,054 bp
  • T to G, chromosome 4 at 128,586,055 bp
  • C to T, chromosome 4 at 137,405,911 bp
  • T to C, chromosome 4 at 141,474,578 bp
  • A to T, chromosome 4 at 149,247,816 bp
  • T to C, chromosome 5 at 33,936,195 bp
  • T to A, chromosome 5 at 65,375,683 bp
  • T to A, chromosome 5 at 90,548,816 bp
  • A to G, chromosome 5 at 122,360,149 bp
  • T to C, chromosome 5 at 124,719,997 bp
  • T to A, chromosome 5 at 147,033,426 bp
  • T to C, chromosome 6 at 24,090,786 bp
  • T to C, chromosome 6 at 116,254,438 bp
  • T to C, chromosome 7 at 4,681,859 bp
  • T to A, chromosome 7 at 15,987,556 bp
  • T to A, chromosome 7 at 19,081,048 bp
  • A to C, chromosome 7 at 62,380,251 bp
  • T to A, chromosome 7 at 86,321,161 bp
  • A to G, chromosome 7 at 88,309,816 bp
  • A to T, chromosome 7 at 104,618,260 bp
  • T to A, chromosome 7 at 105,709,321 bp
  • T to C, chromosome 7 at 141,611,345 bp
  • T to C, chromosome 8 at 4,234,141 bp
  • C to T, chromosome 8 at 22,540,380 bp
  • T to A, chromosome 8 at 45,042,294 bp
  • A to T, chromosome 8 at 61,516,701 bp
  • T to A, chromosome 8 at 84,176,186 bp
  • G to A, chromosome 8 at 95,096,359 bp
  • A to T, chromosome 9 at 110,084,445 bp
  • G to T, chromosome 10 at 52,319,862 bp
  • C to T, chromosome 10 at 127,556,027 bp
  • C to A, chromosome 11 at 43,442,653 bp
  • T to C, chromosome 11 at 51,624,495 bp
  • T to A, chromosome 11 at 53,465,167 bp
  • T to A, chromosome 11 at 53,583,362 bp
  • T to C, chromosome 11 at 53,988,892 bp
  • T to C, chromosome 11 at 87,602,800 bp
  • T to G, chromosome 11 at 95,265,219 bp
  • C to T, chromosome 11 at 100,379,565 bp
  • T to A, chromosome 11 at 103,500,664 bp
  • T to C, chromosome 11 at 117,749,263 bp
  • T to A, chromosome 12 at 5,140,710 bp
  • T to C, chromosome 13 at 92,021,717 bp
  • A to G, chromosome 13 at 119,366,431 bp
  • C to T, chromosome 14 at 20,352,709 bp
  • T to A, chromosome 14 at 33,955,835 bp
  • T to C, chromosome 14 at 34,514,426 bp
  • T to A, chromosome 14 at 64,021,996 bp
  • T to C, chromosome 15 at 44,529,519 bp
  • G to T, chromosome 15 at 85,132,136 bp
  • A to G, chromosome 15 at 101,135,523 bp
  • A to G, chromosome 16 at 15,675,365 bp
  • A to G, chromosome 16 at 20,298,159 bp
  • T to C, chromosome 16 at 36,041,556 bp
  • T to C, chromosome 16 at 46,448,902 bp
  • A to G, chromosome 16 at 58,456,352 bp
  • C to T, chromosome 17 at 20,847,059 bp
  • G to A, chromosome 17 at 78,345,980 bp
  • T to G, chromosome 18 at 49,864,508 bp
  • C to T, chromosome 18 at 49,893,674 bp
  • T to A, chromosome 18 at 77,979,219 bp
  • A to G, chromosome 19 at 33,964,873 bp
  • T to C, chromosome 19 at 46,856,923 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8971 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068805-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.