Strain Name:
C57BL/6J-MtgxR8993Btlr/Mmmh
Stock Number:
068824-MU
Citation ID:
RRID:MMRRC_068824-MU
Other Names:
R8993 (G1)
Major Collection:

Strain Information

Itk
Name: IL2 inducible T cell kinase
Synonyms: Tsk, Tcsk, Emt
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16428
HGNC: HGNC:6171
Homologene: 4051
Gatad2a
Name: GATA zinc finger domain containing 2A
Synonyms: 1110066C11Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234366
Homologene: 9766
Pde1b
Name: phosphodiesterase 1B, Ca2+-calmodulin dependent
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 18574
VEGA: 15
HGNC: HGNC:8775
Homologene: 37370
Ttc3
Name: tetratricopeptide repeat domain 3
Synonyms: D16Ium21e, 2610202A04Rik, D16Ium21, TPRD
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 22129
Homologene: 2487
Braf
Name: Braf transforming gene
Synonyms: Braf-2, Braf2, D6Ertd631e, 9930012E13Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 109880
HGNC: HGNC:1097
Homologene: 3197
Dnah7a
Name: dynein, axonemal, heavy chain 7A
Synonyms: LOC381341, Dnahc7, Dnahc7a
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 627872
Homologene: 41287
Adcy4
Name: adenylate cyclase 4
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 104110
VEGA: 14
HGNC: HGNC:235
Homologene: 23149
Iqsec3
Name: IQ motif and Sec7 domain 3
Synonyms: BRAG3, synarfGEF
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 243621
Homologene: 46091
Bcan
Name: brevican
Synonyms: Cspg7
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 12032
Homologene: 7244
Bod1l
Name: biorientation of chromosomes in cell division 1-like
Synonyms: A230054D04Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 665775
Homologene: 45109
Celf6
Name: CUGBP, Elav-like family member 6
Synonyms: Brunol6, 6330569O16Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 76183
Homologene: 129318
Mpp3
Name: membrane protein, palmitoylated 3 (MAGUK p55 subfamily member 3)
Synonyms: Dlgh3
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 13384
HGNC: HGNC:7221
Homologene: 31065
Crmp1
Name: collapsin response mediator protein 1
Synonyms: DRP-1, Ulip3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 12933
HGNC: HGNC:2365
Homologene: 20347
Pnpla7
Name: patatin-like phospholipase domain containing 7
Synonyms: NRE, E430013P11Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241274
Homologene: 62431
Lonrf1
Name: LON peptidase N-terminal domain and ring finger 1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244421
Homologene: 34980
Cdkl2
Name: cyclin dependent kinase like 2
Synonyms: KKIAMRE, Kkm, 5330436L21Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 53886
HGNC: HGNC:1782
Homologene: 55793
Col1a2
Name: collagen, type I, alpha 2
Synonyms: Cola-2, Col1a-2, Cola2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 12843
HGNC: HGNC:2198
Homologene: 69
Lama2
Name: laminin, alpha 2
Synonyms: merosin, mer
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 16773
HGNC: HGNC:6482
Homologene: 37306
Ttn
Name: titin
Synonyms: 2310074I15Rik, D330041I19Rik, 2310057K23Rik, connectin, 2310036G12Rik, shru, D830007G01Rik, L56, 1100001C23Rik, mdm
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22138
Homologene: 130650
Kif27
Name: kinesin family member 27
Synonyms: 4930517I18Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 75050
VEGA: 13
Homologene: 69235
Slc6a18
Name: solute carrier family 6 (neurotransmitter transporter), member 18
Synonyms: Xtrp2, XT2, D630001K16Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 22598
VEGA: 13
Homologene: 40785
Dock10
Name: dedicator of cytokinesis 10
Synonyms: Jr5, ZIZ3, 9330153B10Rik, Zizimin3, A630054M16Rik, Jr4
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 210293
Homologene: 45952
Tm7sf2
Name: transmembrane 7 superfamily member 2
Synonyms: 3110041O18Rik, ANG1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 73166
Homologene: 68305
Cntn1
Name: contactin 1
Synonyms: CNTN, F3cam, usl
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 12805
HGNC: HGNC:2171
Homologene: 7274
Slc5a4a
Name: solute carrier family 5, member 4a
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 64452
VEGA: 10
Pde4dip
Name: phosphodiesterase 4D interacting protein (myomegalin)
Synonyms: D130016K21Rik, D3Bwg1078e, 4732458A06Rik, 9430063L05Rik, Usmg4
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 83679
Homologene: 66961
Ubtfl1
Name: upstream binding transcription factor, RNA polymerase I-like 1
Synonyms: Hmgpi, B020006M18Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 546118
Homologene: 110115
Foxa2
Name: forkhead box A2
Synonyms: HNF3-beta, Hnf-3b, Hnf3b, HNF3beta, Tcf3b, Tcf-3b
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 15376
HGNC: HGNC:5022
Homologene: 7762
Tbx18
Name: T-box18
Synonyms: 2810012F10Rik, 2810404D13Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 76365
Homologene: 11384
Arhgef26
Name: Rho guanine nucleotide exchange factor 26
Synonyms: 8430436L14Rik, 4631416L12Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 622434
Homologene: 9204
Wdr46
Name: WD repeat domain 46
Synonyms: 2310007I04Rik, Bing4
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 57315
Homologene: 3981
Zfp426
Name: zinc finger protein 426
Synonyms: KRAB1, 2900057C04Rik, Zfp68-rs1, Zfo61
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235028
Homologene: 23435
Vmn2r118
Name: vomeronasal 2, receptor 118
Synonyms: EG383258, Vmn2r119, EG668547
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 383258
Homologene: 129687
Ttll11
Name: tubulin tyrosine ligase-like family, member 11
Synonyms: 4932702F08Rik, D2Ertd624e, 4933424A20Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 74410
Homologene: 77534
BB014433
Name: expressed sequence BB014433
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 434285
Nlrx1
Name: NLR family member X1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 270151
VEGA: 9
Homologene: 11623
Rmnd1
Name: required for meiotic nuclear division 1 homolog
Synonyms: 0610042C05Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 66084
Homologene: 5675
Poglut2
Name: protein O-glucosyltransferase 2
Synonyms: 5730416C13Rik, 1810049A15Rik, EP58, Kdelc1, Kdel1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 72050
Homologene: 11367
Arhgef16
Name: Rho guanine nucleotide exchange factor 16
Synonyms: Neuroblastoma
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230972
Homologene: 82484
Sypl1
Name: synaptophysin like 1
Synonyms: Pan I, Sypl, D12Ertd446e, Pphn
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 19027
Homologene: 4915
Nat1
Name: N-acetyl transferase 1
Synonyms: Nat-1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 17960
Homologene: 115468
Grid1
Name: glutamate receptor, ionotropic, delta 1
Synonyms: GluRdelta1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 14803
HGNC: HGNC:4575
Homologene: 69017
Nipal2
Name: NIPA-like domain containing 2
Synonyms: Npal2, 9330161F08Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223473
VEGA: 15
Homologene: 41586
Acaa1a
Name: acetyl-Coenzyme A acyltransferase 1A
Synonyms: Acaa1, peroxisomal 3-ketoacyl-CoA thiolase, PTL, D9Ertd25e, thiolase A
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 113868
HGNC: HGNC:82
Homologene: 37497
Trbv21
Name: T cell receptor beta, variable 21
Synonyms: Gm16778, Tcrb-V19
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 100124686
Spesp1
Name: sperm equatorial segment protein 1
Synonyms: 4921508E09Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 66712
VEGA: 9
Homologene: 49838
Qdpr
Name: quinoid dihydropteridine reductase
Synonyms: 2610008L04Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 110391
HGNC: HGNC:9752
Homologene: 271
Golim4
Name: golgi integral membrane protein 4
Synonyms: Golph4, 3110027H23Rik, P138, GPP130
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 73124
Homologene: 8716
Gimap4
Name: GTPase, IMAP family member 4
Synonyms: E430007K16Rik, Ian1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 107526
Homologene: 75084
Cmtm4
Name: CKLF-like MARVEL transmembrane domain containing 4
Synonyms: Cklfsf4
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 97487
Homologene: 45211
Eif1ad15
Name: eukaryotic translation initiation factor 1A domain containing 15
Synonyms: Gm5039
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 266459
VEGA: 12
Fam24a
Name: family with sequence similarity 24, member A
Synonyms: 1700063I17Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 68223
Homologene: 135971
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 11,545,267 bp
  • G to T, chromosome 1 at 44,112,764 bp
  • A to T, chromosome 1 at 53,504,103 bp
  • G to T, chromosome 1 at 80,574,171 bp
  • A to G, chromosome 2 at 25,053,419 bp
  • T to C, chromosome 2 at 35,817,801 bp
  • T to C, chromosome 2 at 76,753,370 bp
  • A to T, chromosome 2 at 148,044,706 bp
  • G to T, chromosome 2 at 176,809,830 bp
  • A to T, chromosome 3 at 62,448,104 bp
  • G to T, chromosome 3 at 75,878,128 bp
  • G to A, chromosome 3 at 87,994,222 bp
  • A to G, chromosome 3 at 97,766,494 bp
  • T to C, chromosome 4 at 144,272,959 bp
  • C to T, chromosome 4 at 154,287,038 bp
  • T to C, chromosome 5 at 37,242,146 bp
  • A to C, chromosome 5 at 41,816,867 bp
  • C to T, chromosome 5 at 45,450,044 bp
  • T to A, chromosome 5 at 92,022,151 bp
  • A to T, chromosome 5 at 113,703,781 bp
  • C to A, chromosome 6 at 4,535,451 bp
  • A to G, chromosome 6 at 39,662,151 bp
  • T to C, chromosome 6 at 41,202,990 bp
  • A to C, chromosome 6 at 48,690,605 bp
  • T to A, chromosome 6 at 121,413,313 bp
  • G to T, chromosome 7 at 43,533,447 bp
  • A to G, chromosome 7 at 131,336,540 bp
  • C to T, chromosome 8 at 15,042,101 bp
  • C to T, chromosome 8 at 23,098,939 bp
  • C to A, chromosome 8 at 36,229,238 bp
  • T to C, chromosome 8 at 67,491,742 bp
  • T to A, chromosome 8 at 69,909,935 bp
  • A to C, chromosome 8 at 104,355,166 bp
  • T to A, chromosome 9 at 18,410,341 bp
  • A to G, chromosome 9 at 20,475,000 bp
  • G to T, chromosome 9 at 44,256,941 bp
  • A to T, chromosome 9 at 59,602,871 bp
  • T to C, chromosome 9 at 62,273,270 bp
  • G to A, chromosome 9 at 87,730,717 bp
  • A to T, chromosome 9 at 119,349,352 bp
  • T to G, chromosome 10 at 4,407,918 bp
  • C to A, chromosome 10 at 27,422,714 bp
  • A to T, chromosome 10 at 76,186,535 bp
  • G to A, chromosome 11 at 46,334,908 bp
  • A to G, chromosome 11 at 102,000,665 bp
  • G to A, chromosome 12 at 32,975,663 bp
  • A to G, chromosome 12 at 88,321,400 bp
  • T to A, chromosome 13 at 58,326,098 bp
  • T to A, chromosome 13 at 73,668,271 bp
  • A to G, chromosome 14 at 35,026,942 bp
  • T to C, chromosome 14 at 55,771,378 bp
  • T to C, chromosome 14 at 55,778,699 bp
  • T to A, chromosome 15 at 34,648,837 bp
  • G to A, chromosome 15 at 92,234,466 bp
  • T to C, chromosome 15 at 101,931,006 bp
  • C to A, chromosome 15 at 103,521,425 bp
  • T to A, chromosome 16 at 94,427,808 bp
  • A to G, chromosome 17 at 33,949,182 bp
  • A to T, chromosome 17 at 55,610,835 bp
  • T to A, chromosome 19 at 6,063,926 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8993 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068824-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.