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Strain Name:
C57BL/6J-MtgxR8993Btlr/Mmmh
Stock Number:
068824-MU
Citation ID:
RRID:MMRRC_068824-MU
Other Names:
R8993 (G1)
Major Collection:

Strain Information

Itk
Name: IL2 inducible T cell kinase
Synonyms: Emt, Tsk, Tcsk
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16428
HGNC: HGNC:6171
Homologene: 4051
Gatad2a
Name: GATA zinc finger domain containing 2A
Synonyms: 1110066C11Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234366
Homologene: 9766
Pde1b
Name: phosphodiesterase 1B, Ca2+-calmodulin dependent
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 18574
VEGA: 15
HGNC: HGNC:8775
Homologene: 37370
Ttc3
Name: tetratricopeptide repeat domain 3
Synonyms: TPRD, D16Ium21, 2610202A04Rik, D16Ium21e
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 22129
Homologene: 2487
Braf
Name: Braf transforming gene
Synonyms: Braf-2, Braf2, 9930012E13Rik, D6Ertd631e
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 109880
HGNC: HGNC:1097
Homologene: 3197
Dnah7a
Name: dynein, axonemal, heavy chain 7A
Synonyms: LOC381341, Dnahc7, Dnahc7a
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 627872
Homologene: 41287
Adcy4
Name: adenylate cyclase 4
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 104110
VEGA: 14
HGNC: HGNC:235
Homologene: 23149
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 11,545,267 bp
  • G to T, chromosome 1 at 44,112,764 bp
  • A to T, chromosome 1 at 53,504,103 bp
  • G to T, chromosome 1 at 80,574,171 bp
  • A to G, chromosome 2 at 25,053,419 bp
  • T to C, chromosome 2 at 35,817,801 bp
  • T to C, chromosome 2 at 76,753,370 bp
  • A to T, chromosome 2 at 148,044,706 bp
  • G to T, chromosome 2 at 176,809,830 bp
  • A to T, chromosome 3 at 62,448,104 bp
  • G to T, chromosome 3 at 75,878,128 bp
  • G to A, chromosome 3 at 87,994,222 bp
  • A to G, chromosome 3 at 97,766,494 bp
  • T to C, chromosome 4 at 144,272,959 bp
  • C to T, chromosome 4 at 154,287,038 bp
  • T to C, chromosome 5 at 37,242,146 bp
  • A to C, chromosome 5 at 41,816,867 bp
  • C to T, chromosome 5 at 45,450,044 bp
  • T to A, chromosome 5 at 92,022,151 bp
  • A to T, chromosome 5 at 113,703,781 bp
  • C to A, chromosome 6 at 4,535,451 bp
  • A to G, chromosome 6 at 39,662,151 bp
  • T to C, chromosome 6 at 41,202,990 bp
  • A to C, chromosome 6 at 48,690,605 bp
  • T to A, chromosome 6 at 121,413,313 bp
  • G to T, chromosome 7 at 43,533,447 bp
  • A to G, chromosome 7 at 131,336,540 bp
  • C to T, chromosome 8 at 15,042,101 bp
  • C to T, chromosome 8 at 23,098,939 bp
  • C to A, chromosome 8 at 36,229,238 bp
  • T to C, chromosome 8 at 67,491,742 bp
  • T to A, chromosome 8 at 69,909,935 bp
  • A to C, chromosome 8 at 104,355,166 bp
  • T to A, chromosome 9 at 18,410,341 bp
  • A to G, chromosome 9 at 20,475,000 bp
  • G to T, chromosome 9 at 44,256,941 bp
  • A to T, chromosome 9 at 59,602,871 bp
  • T to C, chromosome 9 at 62,273,270 bp
  • G to A, chromosome 9 at 87,730,717 bp
  • A to T, chromosome 9 at 119,349,352 bp
  • T to G, chromosome 10 at 4,407,918 bp
  • C to A, chromosome 10 at 27,422,714 bp
  • A to T, chromosome 10 at 76,186,535 bp
  • G to A, chromosome 11 at 46,334,908 bp
  • A to G, chromosome 11 at 102,000,665 bp
  • G to A, chromosome 12 at 32,975,663 bp
  • A to G, chromosome 12 at 88,321,400 bp
  • T to A, chromosome 13 at 58,326,098 bp
  • T to A, chromosome 13 at 73,668,271 bp
  • A to G, chromosome 14 at 35,026,942 bp
  • T to C, chromosome 14 at 55,771,378 bp
  • T to C, chromosome 14 at 55,778,699 bp
  • T to A, chromosome 15 at 34,648,837 bp
  • G to A, chromosome 15 at 92,234,466 bp
  • T to C, chromosome 15 at 101,931,006 bp
  • C to A, chromosome 15 at 103,521,425 bp
  • T to A, chromosome 16 at 94,427,808 bp
  • A to G, chromosome 17 at 33,949,182 bp
  • A to T, chromosome 17 at 55,610,835 bp
  • T to A, chromosome 19 at 6,063,926 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8993 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068824-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.