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Strain Name:
C57BL/6J-MtgxR8995Btlr/Mmmh
Stock Number:
068826-MU
Citation ID:
RRID:MMRRC_068826-MU
Other Names:
R8995 (G1)
Major Collection:

Strain Information

Shprh
Name: SNF2 histone linker PHD RING helicase
Synonyms: 2610103K11Rik, D230017O13Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 268281
Homologene: 6489
Cdon
Name: cell adhesion molecule-related/down-regulated by oncogenes
Synonyms: CAM-related/down-regulated by oncogenes, CDO
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 57810
Homologene: 22996
Mpc1
Name: mitochondrial pyruvate carrier 1
Synonyms: 0610006G08Rik, 3830411I18Rik, Brp44l
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 55951
Homologene: 9384
Rfx3
Name: regulatory factor X, 3 (influences HLA class II expression)
Synonyms: MRFX3, C230093O12Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 19726
HGNC: HGNC:9984
Homologene: 7917
Zfand5
Name: zinc finger, AN1-type domain 5
Synonyms: 2310057A04Rik, Zfp216, 5830475F03Rik, Za20d2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 22682
Homologene: 21215
Ddx50
Name: DExD box helicase 50
Synonyms: GU2, RH-II/Gubeta, 4933429B04Rik, 8430408E17Rik, DEAD (Asp-Glu-Ala-Asp) box polypeptide 50
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 94213
VEGA: 10
Homologene: 56986
Ndc80
Name: NDC80 kinetochore complex component
Synonyms: HEC1, 2610020P18Rik, Kntc2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 67052
VEGA: 17
Homologene: 38141
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 63,178,470 bp
  • A to G, chromosome 1 at 65,205,587 bp
  • A to G, chromosome 1 at 87,805,330 bp
  • T to A, chromosome 1 at 134,971,920 bp
  • T to C, chromosome 1 at 153,332,247 bp
  • T to A, chromosome 1 at 156,525,303 bp
  • A to G, chromosome 1 at 175,908,561 bp
  • A to T, chromosome 1 at 175,964,014 bp
  • T to G, chromosome 1 at 188,444,653 bp
  • A to T, chromosome 1 at 191,011,620 bp
  • A to G, chromosome 2 at 4,744,292 bp
  • G to A, chromosome 2 at 34,799,830 bp
  • C to A, chromosome 2 at 91,273,584 bp
  • T to C, chromosome 2 at 119,072,509 bp
  • G to A, chromosome 2 at 120,866,553 bp
  • A to C, chromosome 2 at 121,925,718 bp
  • C to T, chromosome 2 at 153,393,966 bp
  • A to G, chromosome 3 at 135,295,228 bp
  • G to T, chromosome 3 at 136,066,503 bp
  • T to A, chromosome 4 at 45,803,413 bp
  • C to T, chromosome 4 at 49,648,437 bp
  • T to A, chromosome 4 at 58,486,954 bp
  • G to A, chromosome 4 at 152,538,888 bp
  • C to T, chromosome 4 at 154,287,038 bp
  • A to G, chromosome 5 at 21,979,579 bp
  • G to T, chromosome 5 at 96,712,556 bp
  • G to A, chromosome 5 at 121,254,359 bp
  • A to G, chromosome 5 at 137,395,620 bp
  • A to T, chromosome 6 at 13,195,997 bp
  • A to G, chromosome 6 at 69,500,527 bp
  • C to A, chromosome 6 at 83,675,909 bp
  • C to A, chromosome 6 at 85,438,847 bp
  • A to G, chromosome 6 at 90,223,775 bp
  • T to C, chromosome 6 at 90,269,686 bp
  • T to C, chromosome 6 at 121,325,053 bp
  • A to G, chromosome 7 at 7,551,193 bp
  • T to A, chromosome 7 at 42,582,648 bp
  • T to A, chromosome 7 at 43,573,374 bp
  • A to T, chromosome 7 at 104,304,325 bp
  • A to T, chromosome 7 at 141,016,704 bp
  • A to T, chromosome 8 at 19,903,424 bp
  • T to C, chromosome 8 at 25,641,153 bp
  • T to A, chromosome 8 at 39,589,419 bp
  • A to T, chromosome 8 at 83,271,551 bp
  • A to G, chromosome 8 at 83,322,686 bp
  • A to G, chromosome 8 at 105,700,499 bp
  • A to G, chromosome 8 at 121,531,025 bp
  • T to C, chromosome 9 at 16,375,602 bp
  • A to G, chromosome 9 at 35,486,797 bp
  • T to C, chromosome 9 at 39,796,017 bp
  • T to A, chromosome 9 at 49,114,594 bp
  • T to A, chromosome 9 at 50,344,778 bp
  • T to A, chromosome 10 at 11,164,830 bp
  • A to G, chromosome 10 at 62,464,679 bp
  • A to G, chromosome 10 at 62,634,083 bp
  • G to A, chromosome 10 at 77,497,469 bp
  • A to T, chromosome 10 at 80,550,261 bp
  • T to C, chromosome 10 at 127,658,642 bp
  • G to A, chromosome 11 at 50,951,060 bp
  • A to T, chromosome 11 at 74,239,794 bp
  • T to C, chromosome 11 at 99,316,556 bp
  • A to T, chromosome 11 at 118,284,999 bp
  • G to A, chromosome 12 at 111,776,910 bp
  • A to G, chromosome 13 at 59,726,607 bp
  • GTT to GT, chromosome 13 at 81,405,338 bp
  • G to A, chromosome 14 at 20,082,937 bp
  • T to A, chromosome 14 at 55,739,939 bp
  • G to A, chromosome 15 at 92,234,466 bp
  • A to G, chromosome 17 at 8,283,952 bp
  • C to T, chromosome 17 at 21,880,191 bp
  • T to C, chromosome 17 at 71,508,603 bp
  • A to G, chromosome 17 at 73,898,374 bp
  • T to A, chromosome 18 at 37,752,177 bp
  • T to A, chromosome 19 at 21,277,023 bp
  • T to A, chromosome 19 at 27,806,325 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8995 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068826-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.