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Strain Name:
C57BL/6J-MtgxR8996Btlr/Mmmh
Stock Number:
068827-MU
Citation ID:
RRID:MMRRC_068827-MU
Other Names:
R8996 (G1)
Major Collection:

Strain Information

Gnb5
Name: guanine nucleotide binding protein (G protein), beta 5
Synonyms: G beta 5, Gbeta5
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 14697
VEGA: 9
HGNC: HGNC:4401
Homologene: 40714
Sf1
Name: splicing factor 1
Synonyms: WBP4, MZFM, CW17R, Zfp162
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 22668
Homologene: 134065
Cxcr4
Name: C-X-C motif chemokine receptor 4
Synonyms: PB-CKR, fusin, CD184, Cmkar4, Sdf1r, b2b220Clo
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12767
HGNC: HGNC:2561
Homologene: 20739
Usp14
Name: ubiquitin specific peptidase 14
Synonyms: dUB-type TGT, 2610005K12Rik, ataxia, ax, NMF375, 2610037B11Rik, nmf375
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 59025
Homologene: 3780
Tcf7l1
Name: transcription factor 7 like 1 (T cell specific, HMG box)
Synonyms: Tcf3
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 21415
Homologene: 7563
Slc12a3
Name: solute carrier family 12, member 3
Synonyms: NCC, TSC
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 20497
Homologene: 287
Stk3
Name: serine/threonine kinase 3
Synonyms: mess1, 0610042I06Rik, Mst2, MST, Ste20
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 56274
Homologene: 48420
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 74,902,188 bp
  • A to G, chromosome 1 at 128,589,801 bp
  • A to G, chromosome 2 at 160,981,623 bp
  • A to G, chromosome 3 at 89,943,389 bp
  • T to A, chromosome 4 at 24,598,884 bp
  • T to C, chromosome 4 at 43,651,998 bp
  • T to C, chromosome 4 at 119,530,888 bp
  • A to G, chromosome 5 at 8,719,069 bp
  • T to A, chromosome 5 at 15,710,888 bp
  • A to T, chromosome 5 at 71,571,703 bp
  • A to G, chromosome 5 at 110,102,578 bp
  • G to A, chromosome 5 at 112,378,039 bp
  • T to A, chromosome 6 at 18,255,946 bp
  • A to C, chromosome 6 at 39,152,852 bp
  • A to T, chromosome 6 at 69,631,790 bp
  • G to T, chromosome 6 at 72,788,580 bp
  • A to G, chromosome 7 at 27,343,419 bp
  • T to C, chromosome 7 at 47,981,197 bp
  • T to C, chromosome 7 at 98,193,592 bp
  • A to G, chromosome 7 at 102,073,311 bp
  • C to T, chromosome 7 at 104,841,682 bp
  • T to A, chromosome 7 at 105,674,035 bp
  • A to G, chromosome 7 at 132,610,755 bp
  • T to C, chromosome 8 at 15,921,105 bp
  • A to T, chromosome 8 at 94,329,435 bp
  • G to A, chromosome 8 at 129,219,535 bp
  • T to C, chromosome 9 at 75,344,523 bp
  • G to A, chromosome 9 at 108,391,710 bp
  • T to C, chromosome 9 at 115,243,997 bp
  • G to T, chromosome 11 at 11,802,953 bp
  • G to A, chromosome 11 at 119,547,568 bp
  • C to T, chromosome 12 at 65,133,858 bp
  • T to C, chromosome 13 at 23,478,661 bp
  • GTT to GT, chromosome 13 at 81,405,338 bp
  • T to A, chromosome 14 at 53,649,790 bp
  • A to G, chromosome 15 at 34,945,062 bp
  • T to C, chromosome 16 at 52,305,751 bp
  • A to T, chromosome 17 at 34,243,557 bp
  • A to G, chromosome 17 at 68,463,002 bp
  • T to C, chromosome 18 at 10,000,521 bp
  • A to G, chromosome 18 at 12,687,079 bp
  • T to A, chromosome 18 at 60,604,158 bp
  • A to T, chromosome 18 at 78,113,696 bp
  • G to A, chromosome 19 at 6,376,411 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8996 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068827-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.