Strain Name:
C57BL/6J-MtgxR8996Btlr/Mmmh
Stock Number:
068827-MU
Citation ID:
RRID:MMRRC_068827-MU
Other Names:
R8996 (G1)
Major Collection:

Strain Information

Gnb5
Name: guanine nucleotide binding protein (G protein), beta 5
Synonyms: Gbeta5, G beta 5
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 14697
VEGA: 9
HGNC: HGNC:4401
Homologene: 40714
Sf1
Name: splicing factor 1
Synonyms: Zfp162, MZFM, CW17R, WBP4
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 22668
Homologene: 134065
Cxcr4
Name: C-X-C motif chemokine receptor 4
Synonyms: Cmkar4, Sdf1r, b2b220Clo, PB-CKR, CD184, fusin
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12767
HGNC: HGNC:2561
Homologene: 20739
Usp14
Name: ubiquitin specific peptidase 14
Synonyms: nmf375, 2610037B11Rik, NMF375, ax, 2610005K12Rik, dUB-type TGT, ataxia
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 59025
Homologene: 3780
Tcf7l1
Name: transcription factor 7 like 1 (T cell specific, HMG box)
Synonyms: Tcf3
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 21415
Homologene: 7563
Slc12a3
Name: solute carrier family 12, member 3
Synonyms: TSC, NCC
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 20497
Homologene: 287
Stk3
Name: serine/threonine kinase 3
Synonyms: Ste20, 0610042I06Rik, Mst2, MST, mess1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 56274
Homologene: 48420
Stt3b
Name: STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae)
Synonyms: 1300006C19Rik, Simp
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 68292
VEGA: 9
Homologene: 7387
Cftr
Name: cystic fibrosis transmembrane conductance regulator
Synonyms: Abcc7
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 12638
HGNC: HGNC:1884
Homologene: 55465
Mms22l
Name: MMS22-like, DNA repair protein
Synonyms: F730047E07Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 212377
Homologene: 18874
Chd6
Name: chromodomain helicase DNA binding protein 6
Synonyms: 6330406J24Rik, 5430439G14Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 71389
Homologene: 32772
Mis18bp1
Name: MIS18 binding protein 1
Synonyms: C79407
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 217653
Homologene: 10147
Nptx1
Name: neuronal pentraxin 1
Synonyms: Np1, D11Bwg1004e
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18164
HGNC: HGNC:7952
Homologene: 1891
Csmd1
Name: CUB and Sushi multiple domains 1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 94109
Homologene: 69536
Dnhd1
Name: dynein heavy chain domain 1
Synonyms: 8030491N06Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 77505
Homologene: 131117
Adgrv1
Name: adhesion G protein-coupled receptor V1
Synonyms: Mgr1, Mass1, Gpr98, VLGR1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 110789
Homologene: 19815
Kdm7a
Name: lysine (K)-specific demethylase 7A
Synonyms: ENSMUSG00000073143, A630082K20Rik, Jhdm1d, Kdm7a
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 338523
Homologene: 25281
Plcxd1
Name: phosphatidylinositol-specific phospholipase C, X domain containing 1
Synonyms: A330045H12Rik, LOC231597
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 403178
Homologene: 138628
Btn2a2
Name: butyrophilin, subfamily 2, member A2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 238555
Homologene: 56012
Cfap65
Name: cilia and flagella associated protein 65
Synonyms: B230363K08Rik, Ccdc108
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 241116
Homologene: 28093
Shkbp1
Name: Sh3kbp1 binding protein 1
Synonyms: SB1, B930062H15Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 192192
Homologene: 26700
L3mbtl4
Name: L3MBTL4 histone methyl-lysine binding protein
Synonyms: D930040M24Rik, A730037L19Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 320858
Homologene: 114383
Lhpp
Name: phospholysine phosphohistidine inorganic pyrophosphate phosphatase
Synonyms: 2310007H09Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 76429
Homologene: 41469
Usp4
Name: ubiquitin specific peptidase 4 (proto-oncogene)
Synonyms: F730026I20Rik, Unp
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 22258
Homologene: 20716
Fignl1
Name: fidgetin-like 1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 60530
Homologene: 11063
Alcam
Name: activated leukocyte cell adhesion molecule
Synonyms: SC1, DM-GRASP, MuSC, BEN, CD166
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 11658
HGNC: HGNC:400
Homologene: 1229
Mrgpra4
Name: MAS-related GPR, member A4
Synonyms: MrgA4
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 235854
Homologene: 131159
Abcb1a
Name: ATP-binding cassette, sub-family B member 1A
Synonyms: P-glycoprotein, mdr-3, Mdr1a, Pgy3, MDR3, Pgp, Pgy-3, Evi32, multiple drug resistant 1a, P-gp
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18671
HGNC: HGNC:40
Homologene: 55496
Synpo
Name: synaptopodin
Synonyms: 9030217H17Rik, 9330140I15Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 104027
Homologene: 5274
H2-Ob
Name: histocompatibility 2, O region beta locus
Synonyms: H2-Ab2, vic1, H-2I, Ob, H2-Ab, A-beta2, A-beta-2, H-2Ob, H2-IAb2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 15002
HGNC: HGNC:4937
Homologene: 1602
Atp8b2
Name: ATPase, class I, type 8B, member 2
Synonyms: Id
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 54667
Homologene: 23226
B3gnt6
Name: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6
Synonyms: Core3, Core-3
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 272411
Homologene: 16339
Frg2f1
Name: FSHD region gene 2 family member 1
Synonyms: AA415398
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 433752
Homologene: 86332
Gabra4
Name: gamma-aminobutyric acid type A receptor subunit alpha 4
Synonyms: Gabra-4
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14397
HGNC: HGNC:4078
Homologene: 631
Ttc39c
Name: tetratricopeptide repeat domain 39C
Synonyms: 2810439F02Rik, 1700008N02Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 72747
Homologene: 124438
Slc14a1
Name: solute carrier family 14 (urea transporter), member 1
Synonyms: UT-B, 3021401A05Rik, 2610507K20Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 108052
Homologene: 9285
Spag8
Name: sperm associated antigen 8
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 433700
Homologene: 8250
Hps4
Name: HPS4, biogenesis of lysosomal organelles complex 3 subunit 2
Synonyms: Hermansky-Pudlak syndrome 4, BLOC-3, C130020P05Rik, 2010205O06Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 192232
Homologene: 11123
Speer4c1
Name: spermatogenesis associated glutamate (E)-rich protein 4C1
Synonyms: SPEER-4C, Speer4c, 4933431D05Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100502967
Homologene: 69402
Xndc1
Name: Xrcc1 N-terminal domain containing 1
Synonyms: Xndr
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 102443350
Homologene: 128476
Igkv4-54
Name: immunoglobulin kappa chain variable 4-54
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 385291
Trav15-2-dv6-2
Name: T cell receptor alpha variable 15-2-DV6-2
Synonyms: Trav15-2/dv6-2
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 436545
Zfp1006
Name: zinc finger protein 1006
Synonyms: 2610044O15Rik8
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 72139
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 74,902,188 bp
  • A to G, chromosome 1 at 128,589,801 bp
  • A to G, chromosome 2 at 160,981,623 bp
  • A to G, chromosome 3 at 89,943,389 bp
  • T to A, chromosome 4 at 24,598,884 bp
  • T to C, chromosome 4 at 43,651,998 bp
  • T to C, chromosome 4 at 119,530,888 bp
  • A to G, chromosome 5 at 8,719,069 bp
  • T to A, chromosome 5 at 15,710,888 bp
  • A to T, chromosome 5 at 71,571,703 bp
  • A to G, chromosome 5 at 110,102,578 bp
  • G to A, chromosome 5 at 112,378,039 bp
  • T to A, chromosome 6 at 18,255,946 bp
  • A to C, chromosome 6 at 39,152,852 bp
  • A to T, chromosome 6 at 69,631,790 bp
  • G to T, chromosome 6 at 72,788,580 bp
  • A to G, chromosome 7 at 27,343,419 bp
  • T to C, chromosome 7 at 47,981,197 bp
  • T to C, chromosome 7 at 98,193,592 bp
  • A to G, chromosome 7 at 102,073,311 bp
  • C to T, chromosome 7 at 104,841,682 bp
  • T to A, chromosome 7 at 105,674,035 bp
  • A to G, chromosome 7 at 132,610,755 bp
  • T to C, chromosome 8 at 15,921,105 bp
  • A to T, chromosome 8 at 94,329,435 bp
  • G to A, chromosome 8 at 129,219,535 bp
  • T to C, chromosome 9 at 75,344,523 bp
  • G to A, chromosome 9 at 108,391,710 bp
  • T to C, chromosome 9 at 115,243,997 bp
  • G to T, chromosome 11 at 11,802,953 bp
  • G to A, chromosome 11 at 119,547,568 bp
  • C to T, chromosome 12 at 65,133,858 bp
  • T to C, chromosome 13 at 23,478,661 bp
  • GTT to GT, chromosome 13 at 81,405,338 bp
  • T to A, chromosome 14 at 53,649,790 bp
  • A to G, chromosome 15 at 34,945,062 bp
  • T to C, chromosome 16 at 52,305,751 bp
  • A to T, chromosome 17 at 34,243,557 bp
  • A to G, chromosome 17 at 68,463,002 bp
  • T to C, chromosome 18 at 10,000,521 bp
  • A to G, chromosome 18 at 12,687,079 bp
  • T to A, chromosome 18 at 60,604,158 bp
  • A to T, chromosome 18 at 78,113,696 bp
  • G to A, chromosome 19 at 6,376,411 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8996 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068827-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.