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Strain Name:
C57BL/6J-MtgxR8997Btlr/Mmmh
Stock Number:
068828-MU
Citation ID:
RRID:MMRRC_068828-MU
Other Names:
R8997 (G1)
Major Collection:

Strain Information

Rnf111
Name: ring finger 111
Synonyms: Arkadia
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 93836
VEGA: 9
Homologene: 9741
Adra2a
Name: adrenergic receptor, alpha 2a
Synonyms: alpha2A, alpha2A-adrenergic receptor, Adra-2a, Adra-2, alpha2A-AR, alpha(2A)AR
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 11551
VEGA: 19
HGNC: HGNC:281
Homologene: 47944
Arid1a
Name: AT-rich interaction domain 1A
Synonyms: Osa1, 1110030E03Rik, Smarcf1, BAF250a
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 93760
Homologene: 21216
Rfc1
Name: replication factor C (activator 1) 1
Synonyms: RFC140, Alp145, 140kDa, Recc1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 19687
HGNC: HGNC:9969
Homologene: 2187
Kdm2b
Name: lysine (K)-specific demethylase 2B
Synonyms: Cxxc2, Jhdm1b, Fbxl10
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 30841
Homologene: 13069
Osbpl8
Name: oxysterol binding protein-like 8
Synonyms: ORP-8, D330025H14Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 237542
VEGA: 10
Homologene: 68813
Trip12
Name: thyroid hormone receptor interactor 12
Synonyms: Gtl6, 1110036I07Rik, 6720416K24Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14897
Homologene: 44226
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 11,545,267 bp
  • G to T, chromosome 1 at 84,793,875 bp
  • T to A, chromosome 1 at 96,867,947 bp
  • A to G, chromosome 1 at 133,090,779 bp
  • C to A, chromosome 1 at 176,955,808 bp
  • C to T, chromosome 2 at 37,800,485 bp
  • A to T, chromosome 2 at 44,825,374 bp
  • A to C, chromosome 2 at 59,858,633 bp
  • A to C, chromosome 2 at 62,334,614 bp
  • C to T, chromosome 2 at 118,786,986 bp
  • A to G, chromosome 2 at 122,357,247 bp
  • A to T, chromosome 2 at 127,754,062 bp
  • C to T, chromosome 2 at 150,959,896 bp
  • T to C, chromosome 3 at 89,887,111 bp
  • T to C, chromosome 3 at 116,117,328 bp
  • A to T, chromosome 3 at 132,734,345 bp
  • T to A, chromosome 3 at 157,016,281 bp
  • T to C, chromosome 4 at 32,773,275 bp
  • T to A, chromosome 4 at 102,933,584 bp
  • T to A, chromosome 4 at 133,694,032 bp
  • G to A, chromosome 4 at 152,538,888 bp
  • A to T, chromosome 5 at 65,275,721 bp
  • A to T, chromosome 5 at 98,275,552 bp
  • C to A, chromosome 5 at 122,880,173 bp
  • A to G, chromosome 6 at 102,204,062 bp
  • G to T, chromosome 6 at 145,621,228 bp
  • A to T, chromosome 7 at 30,038,175 bp
  • G to T, chromosome 8 at 38,660,740 bp
  • T to A, chromosome 8 at 88,252,395 bp
  • A to T, chromosome 9 at 7,129,003 bp
  • T to C, chromosome 9 at 18,607,467 bp
  • G to A, chromosome 9 at 20,594,430 bp
  • T to C, chromosome 9 at 27,048,281 bp
  • A to T, chromosome 9 at 70,476,263 bp
  • T to C, chromosome 10 at 80,586,636 bp
  • A to G, chromosome 10 at 82,061,156 bp
  • T to C, chromosome 10 at 111,255,714 bp
  • G to A, chromosome 10 at 128,396,479 bp
  • C to T, chromosome 11 at 59,043,803 bp
  • T to A, chromosome 11 at 72,317,640 bp
  • A to G, chromosome 12 at 64,473,984 bp
  • A to T, chromosome 12 at 85,320,791 bp
  • T to G, chromosome 13 at 16,026,522 bp
  • GTT to GT, chromosome 13 at 81,405,338 bp
  • A to T, chromosome 13 at 120,240,979 bp
  • T to C, chromosome 14 at 23,462,969 bp
  • T to A, chromosome 14 at 55,972,744 bp
  • G to A, chromosome 15 at 92,234,466 bp
  • A to T, chromosome 16 at 21,152,937 bp
  • A to T, chromosome 17 at 48,360,438 bp
  • A to G, chromosome 17 at 70,516,533 bp
  • A to T, chromosome 18 at 10,510,747 bp
  • C to A, chromosome 18 at 37,681,080 bp
  • T to C, chromosome 19 at 54,047,298 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8997 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068828-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.