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Strain Name:
C57BL/6J-MtgxR9016Btlr/Mmmh
Stock Number:
068846-MU
Citation ID:
RRID:MMRRC_068846-MU
Other Names:
R9016 (G1)
Major Collection:

Strain Information

Hgf
Name: hepatocyte growth factor
Synonyms: scatter factor, NK1, SF/HGF, HGF/SF, NK2, C230052L06Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 15234
HGNC: HGNC:4893
Homologene: 503
Spen
Name: spen family transcription repressor
Synonyms: Mint
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 56381
Homologene: 124461
Taf8
Name: TATA-box binding protein associated factor 8
Synonyms: Taf8, Tbn
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 63856
VEGA: 17
Homologene: 11094
Slc4a7
Name: solute carrier family 4, sodium bicarbonate cotransporter, member 7
Synonyms: E430014N10Rik, NBC3, NBCn1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 218756
VEGA: 14
Homologene: 2680
Agap1
Name: ArfGAP with GTPase domain, ankyrin repeat and PH domain 1
Synonyms: Ggap1, Centg2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 347722
Homologene: 56689
Dnmt1
Name: DNA methyltransferase 1
Synonyms: MTase, Dnmt1o, Cxxc9, MommeD2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 13433
VEGA: 9
HGNC: HGNC:2976
Homologene: 124071
Dennd2b
Name: DENN domain containing 2B
Synonyms: 2610305K15Rik, 2010004M01Rik, St5, Denn2b
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 76954
Homologene: 3951
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 59,815,301 bp
  • T to C, chromosome 1 at 84,695,505 bp
  • T to C, chromosome 1 at 89,766,466 bp
  • C to T, chromosome 1 at 93,025,673 bp
  • G to A, chromosome 1 at 105,827,129 bp
  • T to C, chromosome 1 at 126,695,754 bp
  • A to T, chromosome 1 at 166,165,161 bp
  • G to A, chromosome 2 at 3,226,397 bp
  • A to G, chromosome 2 at 91,036,827 bp
  • G to A, chromosome 3 at 28,638,395 bp
  • A to T, chromosome 3 at 59,255,873 bp
  • T to C, chromosome 3 at 96,920,205 bp
  • A to T, chromosome 3 at 142,544,109 bp
  • T to C, chromosome 4 at 57,637,857 bp
  • T to G, chromosome 4 at 64,017,094 bp
  • C to G, chromosome 4 at 88,835,809 bp
  • T to C, chromosome 4 at 117,268,254 bp
  • C to T, chromosome 4 at 141,473,627 bp
  • T to C, chromosome 4 at 143,697,329 bp
  • A to G, chromosome 4 at 147,613,047 bp
  • T to C, chromosome 5 at 16,618,958 bp
  • G to T, chromosome 5 at 24,383,641 bp
  • A to T, chromosome 5 at 96,636,064 bp
  • T to A, chromosome 5 at 108,388,726 bp
  • T to C, chromosome 5 at 109,294,243 bp
  • C to A, chromosome 5 at 137,800,078 bp
  • T to C, chromosome 5 at 139,758,571 bp
  • G to A, chromosome 6 at 83,368,271 bp
  • A to G, chromosome 6 at 90,382,272 bp
  • A to T, chromosome 6 at 97,185,733 bp
  • A to C, chromosome 6 at 121,781,841 bp
  • A to G, chromosome 7 at 3,616,073 bp
  • T to C, chromosome 7 at 6,317,405 bp
  • C to T, chromosome 7 at 25,225,839 bp
  • A to T, chromosome 7 at 29,191,737 bp
  • T to C, chromosome 7 at 30,855,029 bp
  • T to G, chromosome 7 at 45,936,564 bp
  • T to A, chromosome 7 at 99,876,255 bp
  • A to T, chromosome 7 at 104,644,621 bp
  • A to C, chromosome 7 at 109,540,435 bp
  • G to A, chromosome 8 at 23,116,248 bp
  • T to G, chromosome 8 at 45,795,737 bp
  • G to A, chromosome 8 at 122,403,224 bp
  • T to C, chromosome 9 at 20,936,559 bp
  • C to T, chromosome 9 at 59,868,144 bp
  • A to T, chromosome 10 at 81,192,432 bp
  • G to A, chromosome 11 at 51,048,111 bp
  • T to C, chromosome 11 at 66,108,030 bp
  • T to G, chromosome 11 at 69,655,703 bp
  • C to T, chromosome 12 at 7,985,408 bp
  • T to C, chromosome 12 at 84,809,693 bp
  • T to C, chromosome 12 at 102,785,158 bp
  • T to A, chromosome 12 at 111,081,499 bp
  • G to A, chromosome 13 at 14,637,608 bp
  • G to T, chromosome 13 at 51,713,758 bp
  • G to A, chromosome 13 at 100,717,618 bp
  • A to G, chromosome 14 at 3,191,110 bp
  • G to A, chromosome 14 at 14,773,241 bp
  • G to C, chromosome 14 at 60,379,927 bp
  • T to A, chromosome 15 at 47,659,042 bp
  • G to A, chromosome 15 at 99,279,735 bp
  • T to A, chromosome 16 at 17,567,440 bp
  • T to A, chromosome 16 at 37,022,797 bp
  • T to C, chromosome 16 at 91,404,185 bp
  • G to T, chromosome 17 at 21,261,707 bp
  • A to C, chromosome 17 at 34,844,664 bp
  • G to A, chromosome 17 at 35,159,868 bp
  • G to A, chromosome 17 at 36,773,334 bp
  • A to T, chromosome 17 at 47,496,602 bp
  • A to T, chromosome 17 at 65,430,783 bp
  • T to A, chromosome 17 at 66,344,067 bp
  • G to A, chromosome 18 at 25,110,079 bp
  • C to A, chromosome 18 at 74,799,083 bp
  • G to C, chromosome 19 at 6,250,081 bp
  • A to T, chromosome 19 at 10,200,942 bp
  • A to C, chromosome 19 at 36,994,305 bp
  • C to T, chromosome X at 142,237,751 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9016 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068846-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.