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Strain Name:
C57BL/6J-MtgxR9043Btlr/Mmmh
Stock Number:
068870-MU
Citation ID:
RRID:MMRRC_068870-MU
Other Names:
R9043 (G1)
Major Collection:

Strain Information

Lamb3
Name: laminin, beta 3
Synonyms: nicein, 125kDa
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16780
HGNC: HGNC:6490
Homologene: 191
Grm1
Name: glutamate receptor, metabotropic 1
Synonyms: mGluR1, Grm1, Gprc1a, nmf373, rcw, 4930455H15Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 14816
HGNC: HGNC:4593
Homologene: 649
Pax2
Name: paired box 2
Synonyms: Pax-2, Opdc
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 18504
HGNC: HGNC:8616
Homologene: 2968
Meis1
Name: Meis homeobox 1
Synonyms: C530044H18Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 17268
HGNC: HGNC:7000
Homologene: 86803
Pzp2
Name: PZP alpha-2-macroglobulin like 2
Synonyms: Pzp
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 11287
Homologene: 104112
Slc4a7
Name: solute carrier family 4, sodium bicarbonate cotransporter, member 7
Synonyms: E430014N10Rik, NBC3, NBCn1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 218756
VEGA: 14
Homologene: 2680
Yes1
Name: YES proto-oncogene 1, Src family tyrosine kinase
Synonyms: Yes
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 22612
Homologene: 55900
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 50,979,620 bp
  • T to A, chromosome 1 at 74,904,688 bp
  • C to T, chromosome 1 at 128,343,494 bp
  • A to T, chromosome 1 at 153,841,821 bp
  • T to A, chromosome 1 at 155,897,482 bp
  • T to C, chromosome 1 at 172,632,617 bp
  • A to T, chromosome 1 at 193,325,611 bp
  • A to T, chromosome 2 at 13,574,438 bp
  • T to A, chromosome 2 at 88,070,639 bp
  • A to T, chromosome 2 at 129,284,972 bp
  • A to C, chromosome 3 at 62,603,939 bp
  • A to T, chromosome 3 at 89,443,487 bp
  • C to A, chromosome 4 at 116,802,951 bp
  • G to A, chromosome 4 at 137,613,685 bp
  • T to A, chromosome 4 at 144,623,220 bp
  • A to G, chromosome 5 at 32,652,968 bp
  • A to T, chromosome 5 at 102,926,159 bp
  • A to T, chromosome 5 at 108,653,195 bp
  • G to A, chromosome 5 at 138,261,627 bp
  • A to G, chromosome 5 at 138,278,931 bp
  • A to T, chromosome 5 at 140,666,055 bp
  • T to C, chromosome 5 at 145,869,185 bp
  • T to G, chromosome 6 at 48,493,280 bp
  • A to T, chromosome 6 at 128,494,779 bp
  • T to A, chromosome 6 at 129,658,778 bp
  • A to T, chromosome 6 at 142,430,333 bp
  • A to G, chromosome 7 at 8,471,905 bp
  • A to G, chromosome 7 at 26,809,831 bp
  • A to T, chromosome 7 at 43,590,181 bp
  • T to A, chromosome 7 at 56,277,441 bp
  • A to T, chromosome 7 at 65,312,931 bp
  • A to G, chromosome 7 at 90,451,541 bp
  • A to C, chromosome 7 at 102,099,492 bp
  • T to C, chromosome 7 at 119,952,049 bp
  • A to G, chromosome 8 at 3,495,810 bp
  • T to A, chromosome 8 at 3,634,478 bp
  • A to G, chromosome 8 at 80,496,317 bp
  • T to A, chromosome 8 at 87,582,610 bp
  • C to T, chromosome 8 at 123,130,212 bp
  • C to T, chromosome 9 at 20,475,012 bp
  • C to T, chromosome 9 at 43,224,011 bp
  • T to C, chromosome 9 at 57,792,617 bp
  • A to T, chromosome 9 at 96,602,717 bp
  • A to T, chromosome 10 at 10,689,312 bp
  • T to C, chromosome 10 at 26,280,254 bp
  • T to G, chromosome 10 at 60,315,699 bp
  • T to C, chromosome 10 at 93,984,165 bp
  • A to G, chromosome 10 at 115,478,438 bp
  • T to C, chromosome 10 at 123,704,389 bp
  • A to T, chromosome 10 at 130,180,285 bp
  • T to A, chromosome 11 at 18,881,916 bp
  • T to A, chromosome 11 at 41,974,835 bp
  • T to C, chromosome 11 at 58,307,724 bp
  • T to A, chromosome 11 at 65,954,854 bp
  • T to C, chromosome 11 at 70,215,694 bp
  • T to G, chromosome 11 at 94,060,259 bp
  • T to C, chromosome 11 at 100,204,638 bp
  • A to G, chromosome 11 at 102,885,859 bp
  • T to A, chromosome 11 at 108,942,968 bp
  • A to G, chromosome 11 at 119,458,913 bp
  • T to C, chromosome 12 at 89,260,482 bp
  • G to A, chromosome 12 at 102,562,795 bp
  • C to T, chromosome 13 at 3,932,708 bp
  • A to T, chromosome 13 at 110,396,660 bp
  • T to C, chromosome 14 at 14,775,048 bp
  • C to T, chromosome 14 at 55,651,754 bp
  • T to C, chromosome 14 at 63,492,829 bp
  • A to C, chromosome 14 at 65,931,621 bp
  • A to G, chromosome 15 at 31,616,955 bp
  • A to G, chromosome 15 at 58,150,608 bp
  • A to G, chromosome 15 at 78,907,249 bp
  • A to T, chromosome 15 at 97,008,924 bp
  • A to G, chromosome 15 at 97,686,562 bp
  • A to T, chromosome 16 at 17,652,718 bp
  • T to A, chromosome 16 at 18,399,702 bp
  • A to C, chromosome 17 at 58,973,540 bp
  • C to G, chromosome 17 at 71,686,704 bp
  • T to C, chromosome 18 at 12,295,913 bp
  • T to C, chromosome 18 at 37,448,798 bp
  • C to A, chromosome 18 at 37,669,910 bp
  • T to A, chromosome 18 at 61,989,890 bp
  • A to G, chromosome 19 at 5,138,936 bp
  • T to A, chromosome 19 at 12,920,303 bp
  • A to T, chromosome 19 at 13,282,710 bp
  • T to A, chromosome 19 at 44,816,060 bp
  • C to T, chromosome X at 37,469,650 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9043 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068870-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.