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Strain Name:
C57BL/6J-MtgxR9055Btlr/Mmmh
Stock Number:
068881-MU
Citation ID:
RRID:MMRRC_068881-MU
Other Names:
R9055 (G1)
Major Collection:

Strain Information

Adamts20
Name: ADAM metallopeptidase with thrombospondin type 1 motif 20
Synonyms: ADAMTS-20, bt
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223838
Homologene: 11808
Irf1
Name: interferon regulatory factor 1
Synonyms: Irf-1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16362
HGNC: HGNC:6116
Homologene: 1658
Fgd4
Name: FYVE, RhoGEF and PH domain containing 4
Synonyms: Frabin-gamma, Frabin-beta, Frabin-alpha, Frabin, 9330209B17Rik, ZFYVE6
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 224014
Homologene: 26727
Ggnbp2
Name: gametogenetin binding protein 2
Synonyms: DIF-3, Zfp403, D330017P12Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217039
Homologene: 11750
Gpr88
Name: G-protein coupled receptor 88
Synonyms: Strg
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 64378
HGNC: HGNC:4539
Homologene: 11104
Alox12b
Name: arachidonate 12-lipoxygenase, 12R type
Synonyms: e-LOX2, Aloxe2, 12R-LOX
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 11686
HGNC: HGNC:430
Homologene: 884
Prep
Name: prolyl endopeptidase
Synonyms: prolyl oligopeptidase, D10Wsu136e, Pop
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 19072
VEGA: 10
HGNC: HGNC:9358
Homologene: 2042
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 6,802,982 bp
  • A to T, chromosome 1 at 12,807,963 bp
  • A to T, chromosome 1 at 75,315,161 bp
  • T to C, chromosome 1 at 90,070,613 bp
  • C to T, chromosome 1 at 91,501,011 bp
  • C to T, chromosome 1 at 93,006,905 bp
  • G to A, chromosome 1 at 151,476,279 bp
  • T to A, chromosome 1 at 166,640,108 bp
  • A to T, chromosome 2 at 84,723,922 bp
  • A to T, chromosome 2 at 111,983,704 bp
  • G to A, chromosome 2 at 150,323,833 bp
  • G to A, chromosome 3 at 40,832,058 bp
  • A to G, chromosome 3 at 82,109,126 bp
  • G to T, chromosome 3 at 98,806,668 bp
  • A to G, chromosome 3 at 116,252,651 bp
  • G to T, chromosome 3 at 121,159,222 bp
  • T to A, chromosome 3 at 130,709,371 bp
  • C to A, chromosome 3 at 130,709,372 bp
  • G to T, chromosome 4 at 58,785,374 bp
  • C to A, chromosome 4 at 113,238,150 bp
  • A to T, chromosome 4 at 132,237,114 bp
  • G to T, chromosome 4 at 135,175,345 bp
  • C to T, chromosome 5 at 26,002,034 bp
  • G to T, chromosome 5 at 31,480,189 bp
  • T to C, chromosome 5 at 74,531,270 bp
  • T to C, chromosome 5 at 90,232,309 bp
  • T to C, chromosome 5 at 100,386,181 bp
  • G to A, chromosome 6 at 40,714,729 bp
  • G to T, chromosome 6 at 47,866,385 bp
  • A to G, chromosome 6 at 71,915,069 bp
  • A to G, chromosome 6 at 72,606,953 bp
  • A to C, chromosome 6 at 78,372,903 bp
  • A to G, chromosome 6 at 90,269,118 bp
  • A to G, chromosome 6 at 102,267,437 bp
  • G to T, chromosome 6 at 130,119,054 bp
  • A to G, chromosome 6 at 133,055,417 bp
  • A to T, chromosome 7 at 25,471,874 bp
  • T to A, chromosome 8 at 13,915,023 bp
  • T to A, chromosome 8 at 69,068,171 bp
  • T to C, chromosome 8 at 123,258,370 bp
  • A to T, chromosome 9 at 6,996,641 bp
  • T to C, chromosome 9 at 38,180,484 bp
  • T to A, chromosome 9 at 57,716,077 bp
  • T to C, chromosome 9 at 108,085,846 bp
  • C to T, chromosome 9 at 119,622,892 bp
  • C to T, chromosome 10 at 45,115,195 bp
  • A to G, chromosome 10 at 79,274,607 bp
  • A to G, chromosome 10 at 88,216,054 bp
  • T to A, chromosome 11 at 53,776,370 bp
  • C to G, chromosome 11 at 69,164,058 bp
  • T to G, chromosome 11 at 70,253,077 bp
  • A to G, chromosome 11 at 72,303,440 bp
  • A to G, chromosome 11 at 78,293,547 bp
  • A to G, chromosome 11 at 84,841,622 bp
  • G to A, chromosome 11 at 104,665,625 bp
  • A to T, chromosome 12 at 85,301,842 bp
  • C to T, chromosome 12 at 112,774,585 bp
  • T to A, chromosome 14 at 7,908,553 bp
  • T to G, chromosome 14 at 53,806,863 bp
  • T to A, chromosome 14 at 70,078,900 bp
  • T to G, chromosome 14 at 73,581,628 bp
  • A to G, chromosome 15 at 94,283,986 bp
  • T to A, chromosome 15 at 101,931,487 bp
  • T to A, chromosome 16 at 16,422,630 bp
  • T to A, chromosome 16 at 22,892,319 bp
  • T to C, chromosome 16 at 57,040,752 bp
  • T to C, chromosome 17 at 13,062,941 bp
  • T to C, chromosome 17 at 94,740,415 bp
  • T to A, chromosome 18 at 37,357,532 bp
  • A to G, chromosome 19 at 3,988,178 bp
  • T to A, chromosome 19 at 57,041,966 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9055 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068881-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.