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Strain Name:
C57BL/6J-MtgxR9101Btlr/Mmmh
Stock Number:
068915-MU
Citation ID:
RRID:MMRRC_068915-MU
Other Names:
R9101 (G1)
Major Collection:

Strain Information

Nefh
Name: neurofilament, heavy polypeptide
Synonyms: NF-H, NF200, NEFH
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 380684
HGNC: HGNC:7737
Homologene: 40755
Ly6a
Name: lymphocyte antigen 6 family member A
Synonyms: Sca-1, TAP, Ly-6A/E, Ly-6E.1, Ly-6A.2, Sca1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 110454
Homologene: 113718
Nsd1
Name: nuclear receptor-binding SET-domain protein 1
Synonyms: KMT3B
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 18193
VEGA: 13
Homologene: 32543
Lars1
Name: leucyl-tRNA synthetase 1
Synonyms: 3110009L02Rik, 2310045K21Rik, Lars
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 107045
VEGA: 18
HGNC: HGNC:6512
Homologene: 7083
Mtarc2
Name: mitochondrial amidoxime reducing component 2
Synonyms: 2810484M10Rik, Mosc2, Marc2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 67247
Homologene: 9904
Bmerb1
Name: bMERB domain containing 1
Synonyms: MINP, 2900011O08Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 67254
Homologene: 13236
Dnmt1
Name: DNA methyltransferase 1
Synonyms: MTase, Dnmt1o, Cxxc9, MommeD2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 13433
VEGA: 9
HGNC: HGNC:2976
Homologene: 124071
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 28,776,659 bp
  • C to T, chromosome 1 at 30,803,945 bp
  • C to T, chromosome 1 at 58,332,637 bp
  • T to C, chromosome 1 at 131,977,437 bp
  • G to A, chromosome 1 at 136,151,155 bp
  • T to A, chromosome 1 at 184,822,490 bp
  • A to G, chromosome 2 at 23,118,437 bp
  • A to G, chromosome 2 at 53,145,243 bp
  • T to A, chromosome 2 at 76,647,840 bp
  • A to G, chromosome 2 at 85,864,179 bp
  • T to C, chromosome 2 at 87,802,580 bp
  • A to T, chromosome 2 at 89,528,377 bp
  • C to T, chromosome 2 at 127,085,970 bp
  • C to T, chromosome 3 at 5,412,138 bp
  • T to A, chromosome 4 at 143,897,076 bp
  • C to T, chromosome 5 at 87,843,471 bp
  • G to A, chromosome 5 at 94,384,040 bp
  • T to A, chromosome 5 at 104,480,364 bp
  • G to C, chromosome 5 at 113,613,982 bp
  • G to T, chromosome 6 at 85,340,693 bp
  • T to A, chromosome 7 at 43,850,781 bp
  • TCCCAGG to T, chromosome 7 at 80,098,331 bp
  • G to A, chromosome 7 at 87,376,240 bp
  • GCG to GCGACGGCGCCG, chromosome 7 at 97,579,907 bp
  • T to C, chromosome 7 at 102,578,930 bp
  • A to T, chromosome 7 at 103,564,473 bp
  • A to G, chromosome 8 at 48,292,151 bp
  • A to T, chromosome 8 at 80,043,962 bp
  • C to A, chromosome 8 at 83,608,490 bp
  • T to C, chromosome 9 at 20,941,543 bp
  • T to A, chromosome 9 at 39,572,509 bp
  • T to A, chromosome 9 at 45,070,685 bp
  • T to C, chromosome 9 at 90,189,741 bp
  • T to C, chromosome 9 at 114,405,241 bp
  • A to G, chromosome 10 at 43,393,949 bp
  • A to G, chromosome 10 at 77,811,816 bp
  • A to G, chromosome 10 at 127,017,004 bp
  • A to G, chromosome 10 at 128,072,129 bp
  • A to G, chromosome 11 at 4,940,925 bp
  • A to G, chromosome 11 at 5,906,516 bp
  • C to A, chromosome 11 at 51,592,914 bp
  • C to T, chromosome 11 at 74,206,496 bp
  • A to G, chromosome 11 at 116,205,736 bp
  • A to G, chromosome 12 at 65,043,304 bp
  • C to A, chromosome 12 at 108,274,658 bp
  • T to C, chromosome 12 at 113,491,756 bp
  • C to T, chromosome 13 at 55,313,546 bp
  • G to A, chromosome 14 at 75,323,602 bp
  • T to A, chromosome 15 at 4,900,453 bp
  • A to T, chromosome 15 at 10,537,575 bp
  • A to T, chromosome 15 at 74,997,570 bp
  • T to A, chromosome 16 at 14,049,395 bp
  • A to G, chromosome 16 at 20,608,849 bp
  • T to C, chromosome 16 at 20,707,229 bp
  • T to A, chromosome 16 at 20,725,807 bp
  • T to C, chromosome 16 at 31,080,927 bp
  • G to A, chromosome 16 at 35,099,786 bp
  • T to C, chromosome 17 at 13,823,444 bp
  • T to A, chromosome 17 at 20,574,209 bp
  • T to A, chromosome 17 at 21,993,411 bp
  • A to G, chromosome 17 at 35,659,088 bp
  • T to C, chromosome 18 at 37,708,340 bp
  • G to A, chromosome 18 at 42,243,877 bp
  • A to C, chromosome 19 at 42,059,908 bp
  • A to T, chromosome 19 at 56,544,306 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9101 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068915-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.