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Strain Name:
C57BL/6J-MtgxR9136Btlr/Mmmh
Stock Number:
068931-MU
Citation ID:
RRID:MMRRC_068931-MU
Other Names:
R9136 (G1)
Major Collection:

Strain Information

Dtnbp1
Name: dystrobrevin binding protein 1
Synonyms: dysbindin, 5430437B18Rik, sdy, Bloc1s8
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 94245
VEGA: 13
Homologene: 12037
Zfp97
Name: zinc finger protein 97
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 22759
Homologene: 133884
Mthfd1
Name: methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthase
Synonyms: Mthfd, DCS, E430024A07Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 108156
VEGA: 12
HGNC: HGNC:7432
Homologene: 55940
Kat2b
Name: K(lysine) acetyltransferase 2B
Synonyms: A930006P13Rik, Pcaf
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18519
HGNC: HGNC:8638
Homologene: 20834
Spen
Name: spen family transcription repressor
Synonyms: Mint
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 56381
Homologene: 124461
Odf2l
Name: outer dense fiber of sperm tails 2-like
Synonyms: 4733401D09Rik, 9630045K08Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 52184
Homologene: 12011
Zfp704
Name: zinc finger protein 704
Synonyms: Gig1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 170753
Homologene: 64370
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 40,543,017 bp
  • G to T, chromosome 1 at 55,089,155 bp
  • A to C, chromosome 1 at 144,536,915 bp
  • A to T, chromosome 1 at 189,671,155 bp
  • A to T, chromosome 2 at 24,927,181 bp
  • A to G, chromosome 2 at 87,308,875 bp
  • A to G, chromosome 2 at 87,634,997 bp
  • T to A, chromosome 2 at 88,973,769 bp
  • C to CGTA, chromosome 2 at 98,663,807 bp
  • C to T, chromosome 2 at 119,528,904 bp
  • T to A, chromosome 2 at 121,236,611 bp
  • T to A, chromosome 2 at 121,457,155 bp
  • C to T, chromosome 2 at 126,597,866 bp
  • G to A, chromosome 2 at 156,746,155 bp
  • T to C, chromosome 3 at 9,444,264 bp
  • T to A, chromosome 3 at 96,152,498 bp
  • T to C, chromosome 3 at 96,643,943 bp
  • C to A, chromosome 3 at 145,145,937 bp
  • T to A, chromosome 4 at 33,404,218 bp
  • C to T, chromosome 4 at 63,392,155 bp
  • T to C, chromosome 4 at 124,902,634 bp
  • T to C, chromosome 4 at 141,522,312 bp
  • A to C, chromosome 5 at 32,828,736 bp
  • A to T, chromosome 5 at 90,244,419 bp
  • G to A, chromosome 5 at 122,121,083 bp
  • T to C, chromosome 5 at 124,090,113 bp
  • T to G, chromosome 6 at 41,461,346 bp
  • C to T, chromosome 6 at 71,874,419 bp
  • T to C, chromosome 6 at 91,043,848 bp
  • A to T, chromosome 6 at 125,599,393 bp
  • A to G, chromosome 6 at 125,982,999 bp
  • A to T, chromosome 7 at 3,823,286 bp
  • T to A, chromosome 7 at 18,726,886 bp
  • A to T, chromosome 7 at 35,776,458 bp
  • G to A, chromosome 7 at 87,376,240 bp
  • G to A, chromosome 7 at 88,040,046 bp
  • A to T, chromosome 7 at 96,823,918 bp
  • A to G, chromosome 7 at 129,603,092 bp
  • C to T, chromosome 8 at 41,279,788 bp
  • T to C, chromosome 8 at 71,652,350 bp
  • G to T, chromosome 8 at 111,008,274 bp
  • C to A, chromosome 9 at 15,922,442 bp
  • T to C, chromosome 9 at 18,754,879 bp
  • C to A, chromosome 9 at 58,260,953 bp
  • A to G, chromosome 9 at 108,947,905 bp
  • G to A, chromosome 10 at 30,691,632 bp
  • T to C, chromosome 10 at 78,285,059 bp
  • A to T, chromosome 11 at 20,648,425 bp
  • A to G, chromosome 11 at 54,394,638 bp
  • A to G, chromosome 11 at 78,074,134 bp
  • A to G, chromosome 11 at 113,806,377 bp
  • C to T, chromosome 12 at 55,303,942 bp
  • C to A, chromosome 12 at 69,586,672 bp
  • T to G, chromosome 12 at 76,303,875 bp
  • A to C, chromosome 13 at 21,501,417 bp
  • A to T, chromosome 13 at 44,931,070 bp
  • C to T, chromosome 13 at 62,855,026 bp
  • T to C, chromosome 14 at 20,531,799 bp
  • C to T, chromosome 14 at 20,665,789 bp
  • T to A, chromosome 14 at 21,007,276 bp
  • A to T, chromosome 14 at 38,397,305 bp
  • T to C, chromosome 14 at 108,911,549 bp
  • A to G, chromosome 15 at 81,769,111 bp
  • A to G, chromosome 15 at 89,336,537 bp
  • T to C, chromosome 16 at 87,594,339 bp
  • C to T, chromosome 16 at 88,896,358 bp
  • G to A, chromosome 17 at 17,144,953 bp
  • A to G, chromosome 17 at 17,529,828 bp
  • A to G, chromosome 17 at 24,377,833 bp
  • T to A, chromosome 17 at 53,629,336 bp
  • T to C, chromosome 17 at 57,146,152 bp
  • A to T, chromosome 19 at 18,957,322 bp
  • A to G, chromosome 19 at 34,022,430 bp
  • A to T, chromosome 19 at 55,277,968 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9136 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068931-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.