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Strain Name:
C57BL/6J-MtgxR9153Btlr/Mmmh
Stock Number:
068940-MU
Citation ID:
RRID:MMRRC_068940-MU
Other Names:
R9153 (G1)
Major Collection:

Strain Information

Slc12a1
Name: solute carrier family 12, member 1
Synonyms: Nkcc2, mBSC1, D630042G03Rik, urehr3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20495
Homologene: 286
Sim1
Name: single-minded family bHLH transcription factor 1
Synonyms: bHLHe14
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 20464
VEGA: 10
Homologene: 3715
Fxyd2
Name: FXYD domain-containing ion transport regulator 2
Synonyms: Atp1g1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 11936
VEGA: 9
HGNC: HGNC:4026
Rgs10
Name: regulator of G-protein signalling 10
Synonyms: 2310010N19Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 67865
HGNC: HGNC:9992
Homologene: 37710
Kctd13
Name: potassium channel tetramerisation domain containing 13
Synonyms: 1500003N18Rik, Poldip1, Pdip1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233877
Homologene: 27800
Jarid2
Name: jumonji and AT-rich interaction domain containing 2
Synonyms: Jmj, jumonji
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 16468
HGNC: HGNC:6196
Homologene: 31279
Secisbp2
Name: SECIS binding protein 2
Synonyms: 2210413N07Rik, SBP2, 2810012K13Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 75420
Homologene: 11415
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 195,092,171 bp
  • A to G, chromosome 2 at 22,399,933 bp
  • A to G, chromosome 2 at 36,052,981 bp
  • A to G, chromosome 2 at 69,965,478 bp
  • A to G, chromosome 2 at 90,684,085 bp
  • G to T, chromosome 2 at 115,867,275 bp
  • A to T, chromosome 2 at 120,925,988 bp
  • T to G, chromosome 2 at 125,161,069 bp
  • G to T, chromosome 2 at 128,782,567 bp
  • G to A, chromosome 2 at 131,192,644 bp
  • A to G, chromosome 2 at 140,057,407 bp
  • T to G, chromosome 2 at 152,691,868 bp
  • T to C, chromosome 2 at 163,203,171 bp
  • C to A, chromosome 2 at 168,184,660 bp
  • A to T, chromosome 2 at 178,283,946 bp
  • A to G, chromosome 2 at 181,147,981 bp
  • T to C, chromosome 3 at 3,508,318 bp
  • A to G, chromosome 4 at 43,308,493 bp
  • A to T, chromosome 4 at 145,899,460 bp
  • A to G, chromosome 4 at 151,027,339 bp
  • C to T, chromosome 4 at 155,386,039 bp
  • A to T, chromosome 5 at 16,835,918 bp
  • A to T, chromosome 5 at 33,431,359 bp
  • T to A, chromosome 5 at 53,135,846 bp
  • C to T, chromosome 5 at 109,086,337 bp
  • G to A, chromosome 6 at 8,022,765 bp
  • A to T, chromosome 6 at 100,611,777 bp
  • A to T, chromosome 6 at 122,040,668 bp
  • T to C, chromosome 6 at 124,540,947 bp
  • G to T, chromosome 6 at 134,641,145 bp
  • A to G, chromosome 6 at 146,758,550 bp
  • G to A, chromosome 7 at 11,401,314 bp
  • T to A, chromosome 7 at 43,475,997 bp
  • C to T, chromosome 7 at 56,293,838 bp
  • A to G, chromosome 7 at 75,609,481 bp
  • A to G, chromosome 7 at 101,999,911 bp
  • T to A, chromosome 7 at 123,174,296 bp
  • A to G, chromosome 7 at 126,942,155 bp
  • G to A, chromosome 7 at 128,374,009 bp
  • T to C, chromosome 8 at 120,753,661 bp
  • G to T, chromosome 9 at 45,408,311 bp
  • T to A, chromosome 9 at 72,944,861 bp
  • T to A, chromosome 9 at 89,944,737 bp
  • A to G, chromosome 10 at 50,895,933 bp
  • A to G, chromosome 10 at 59,367,290 bp
  • T to C, chromosome 10 at 107,580,265 bp
  • T to A, chromosome 10 at 129,647,437 bp
  • A to T, chromosome 11 at 50,297,335 bp
  • A to T, chromosome 11 at 76,904,642 bp
  • A to G, chromosome 11 at 86,799,539 bp
  • G to A, chromosome 11 at 89,412,019 bp
  • A to T, chromosome 11 at 110,216,655 bp
  • A to C, chromosome 11 at 115,984,053 bp
  • C to T, chromosome 11 at 120,815,670 bp
  • A to C, chromosome 12 at 31,660,811 bp
  • A to T, chromosome 12 at 65,059,897 bp
  • T to C, chromosome 13 at 44,911,202 bp
  • A to G, chromosome 13 at 51,679,819 bp
  • T to A, chromosome 13 at 95,708,039 bp
  • A to G, chromosome 13 at 114,865,405 bp
  • T to A, chromosome 13 at 119,893,754 bp
  • T to A, chromosome 14 at 26,814,612 bp
  • T to C, chromosome 14 at 31,174,825 bp
  • T to A, chromosome 14 at 70,522,210 bp
  • G to T, chromosome 15 at 76,180,525 bp
  • A to G, chromosome 15 at 77,955,587 bp
  • G to A, chromosome 15 at 98,543,278 bp
  • G to A, chromosome 16 at 56,186,123 bp
  • T to A, chromosome 16 at 93,587,834 bp
  • T to C, chromosome 17 at 21,657,606 bp
  • G to A, chromosome 17 at 25,163,178 bp
  • T to C, chromosome 18 at 7,286,733 bp
  • T to A, chromosome 18 at 37,721,078 bp
  • T to A, chromosome 18 at 46,607,969 bp
  • T to C, chromosome 18 at 58,598,733 bp
  • A to G, chromosome 19 at 41,847,367 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9153 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068940-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.