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Strain Name:
C57BL/6J-MtgxR9223Btlr/Mmmh
Stock Number:
068959-MU
Citation ID:
RRID:MMRRC_068959-MU
Other Names:
R9223 (G1)
Major Collection:

Strain Information

Tfap2b
Name: transcription factor AP-2 beta
Synonyms: AP-2(beta), E130018K07Rik, Tcfap2b
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 21419
Homologene: 20688
Thbs4
Name: thrombospondin 4
Synonyms: TSP-4, TSP4
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 21828
Homologene: 20691
Ccn2
Name: cellular communication network factor 2
Synonyms: Hcs24, hypertrophic chondrocyte-specific gene product 24, Fisp12, Ccn2, Ctgf
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 14219
HGNC: HGNC:2500
Homologene: 1431
Chst5
Name: carbohydrate sulfotransferase 5
Synonyms: I-GlcNAc6ST, GST-4
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 56773
Homologene: 56927
Serinc3
Name: serine incorporator 3
Synonyms: TMS-1, DIFF33, AIGP1, Tde1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 26943
Homologene: 38230
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 19,212,425 bp
  • T to A, chromosome 1 at 46,322,260 bp
  • T to C, chromosome 1 at 134,879,638 bp
  • A to T, chromosome 1 at 157,107,866 bp
  • T to A, chromosome 1 at 173,937,871 bp
  • TTA to TTATA, chromosome 2 at 28,559,429 bp
  • T to C, chromosome 2 at 36,937,899 bp
  • A to T, chromosome 2 at 79,341,330 bp
  • G to T, chromosome 2 at 86,255,200 bp
  • T to A, chromosome 2 at 112,021,172 bp
  • T to A, chromosome 2 at 112,156,128 bp
  • A to G, chromosome 2 at 163,636,892 bp
  • A to T, chromosome 2 at 181,006,735 bp
  • A to T, chromosome 3 at 79,827,657 bp
  • A to C, chromosome 3 at 94,175,643 bp
  • A to T, chromosome 3 at 107,104,990 bp
  • A to G, chromosome 4 at 81,284,630 bp
  • G to A, chromosome 4 at 109,982,582 bp
  • A to G, chromosome 4 at 126,590,618 bp
  • T to A, chromosome 5 at 6,771,496 bp
  • G to T, chromosome 5 at 34,905,348 bp
  • A to T, chromosome 5 at 100,798,437 bp
  • A to T, chromosome 5 at 123,646,274 bp
  • C to T, chromosome 5 at 142,567,911 bp
  • T to C, chromosome 6 at 39,401,249 bp
  • C to A, chromosome 7 at 49,552,851 bp
  • T to G, chromosome 7 at 57,816,404 bp
  • C to A, chromosome 7 at 102,184,960 bp
  • A to G, chromosome 7 at 106,914,773 bp
  • A to G, chromosome 7 at 131,326,140 bp
  • A to G, chromosome 7 at 139,921,441 bp
  • A to G, chromosome 8 at 25,037,032 bp
  • A to G, chromosome 8 at 26,032,524 bp
  • C to A, chromosome 8 at 111,890,860 bp
  • A to G, chromosome 9 at 46,155,474 bp
  • A to C, chromosome 9 at 89,085,610 bp
  • C to A, chromosome 9 at 101,942,994 bp
  • T to C, chromosome 9 at 109,195,048 bp
  • T to C, chromosome 10 at 24,595,958 bp
  • ATTTT to ATTTTT, chromosome 10 at 64,089,256 bp
  • T to A, chromosome 10 at 107,854,344 bp
  • A to G, chromosome 11 at 29,706,778 bp
  • A to G, chromosome 11 at 61,265,211 bp
  • A to T, chromosome 11 at 70,226,574 bp
  • A to T, chromosome 11 at 99,985,245 bp
  • G to A, chromosome 11 at 105,329,267 bp
  • A to G, chromosome 11 at 107,619,092 bp
  • T to A, chromosome 12 at 65,102,584 bp
  • C to T, chromosome 12 at 66,568,860 bp
  • A to G, chromosome 12 at 104,217,185 bp
  • A to G, chromosome 13 at 12,404,921 bp
  • T to A, chromosome 13 at 49,661,262 bp
  • C to T, chromosome 13 at 92,761,490 bp
  • C to T, chromosome 13 at 100,227,676 bp
  • T to A, chromosome 16 at 57,142,803 bp
  • A to T, chromosome 17 at 23,401,167 bp
  • C to T, chromosome 17 at 38,131,121 bp
  • A to T, chromosome 17 at 46,248,872 bp
  • A to T, chromosome 17 at 88,015,696 bp
  • A to T, chromosome 18 at 35,536,619 bp
  • A to G, chromosome 18 at 37,815,632 bp
  • A to G, chromosome 19 at 44,040,745 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9223 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068959-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.