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Strain Name:
C57BL/6J-MtgxR9166Btlr/Mmmh
Stock Number:
069023-MU
Citation ID:
RRID:MMRRC_069023-MU
Other Names:
R9166 (G1)
Major Collection:

Strain Information

Kera
Name: keratocan
Synonyms: SLRR2B, CNA2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 16545
VEGA: 10
HGNC: HGNC:6309
Homologene: 5106
Nudc
Name: nudC nuclear distribution protein
Synonyms: NudC, Silg92
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18221
HGNC: HGNC:8045
Homologene: 4812
Dlc1
Name: deleted in liver cancer 1
Synonyms: p122-RhoGAP, STARD12, Arhgap7, A730069N07Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 50768
HGNC: HGNC:2897
Homologene: 4442
Aopep
Name: aminopeptidase O
Synonyms: ApO, 2010111I01Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 72061
HGNC: HGNC:1361
Homologene: 66273
Lrrc59
Name: leucine rich repeat containing 59
Synonyms: C78668
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 98238
Homologene: 10229
Prtg
Name: protogenin
Synonyms: A230098A12Rik, Igdcc5
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235472
Homologene: 54453
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • C to A, chromosome 1 at 191,396,307 bp (GRCm38)
  • T to C, chromosome 2 at 13,574,745 bp (GRCm38)
  • T to C, chromosome 2 at 36,055,012 bp (GRCm38)
  • T to A, chromosome 2 at 89,788,947 bp (GRCm38)
  • T to C, chromosome 2 at 158,432,922 bp (GRCm38)
  • G to T, chromosome 2 at 164,190,273 bp (GRCm38)
  • T to C, chromosome 2 at 165,290,518 bp (GRCm38)
  • A to G, chromosome 2 at 180,464,241 bp (GRCm38)
  • A to G, chromosome 3 at 10,088,789 bp (GRCm38)
  • T to A, chromosome 3 at 36,987,367 bp (GRCm38)
  • C to A, chromosome 3 at 133,468,172 bp (GRCm38)
  • T to A, chromosome 3 at 157,999,720 bp (GRCm38)
  • G to C, chromosome 4 at 43,006,800 bp (GRCm38)
  • C to T, chromosome 4 at 131,797,869 bp (GRCm38)
  • T to C, chromosome 4 at 133,545,854 bp (GRCm38)
  • T to C, chromosome 4 at 137,542,874 bp (GRCm38)
  • A to G, chromosome 5 at 31,241,001 bp (GRCm38)
  • G to T, chromosome 5 at 92,298,221 bp (GRCm38)
  • T to C, chromosome 5 at 120,524,031 bp (GRCm38)
  • A to C, chromosome 5 at 121,308,627 bp (GRCm38)
  • A to T, chromosome 5 at 137,113,897 bp (GRCm38)
  • A to T, chromosome 5 at 138,065,519 bp (GRCm38)
  • A to T, chromosome 6 at 37,957,139 bp (GRCm38)
  • G to T, chromosome 6 at 82,913,645 bp (GRCm38)
  • T to C, chromosome 6 at 84,149,977 bp (GRCm38)
  • T to C, chromosome 6 at 90,466,970 bp (GRCm38)
  • A to G, chromosome 6 at 147,019,482 bp (GRCm38)
  • G to A, chromosome 7 at 30,242,449 bp (GRCm38)
  • A to T, chromosome 7 at 41,864,007 bp (GRCm38)
  • A to G, chromosome 7 at 51,936,575 bp (GRCm38)
  • C to T, chromosome 7 at 81,332,871 bp (GRCm38)
  • T to C, chromosome 7 at 118,510,142 bp (GRCm38)
  • A to G, chromosome 7 at 123,187,401 bp (GRCm38)
  • G to A, chromosome 7 at 134,298,319 bp (GRCm38)
  • A to G, chromosome 7 at 141,264,753 bp (GRCm38)
  • C to T, chromosome 8 at 36,599,435 bp (GRCm38)
  • A to C, chromosome 8 at 53,605,687 bp (GRCm38)
  • A to G, chromosome 9 at 50,536,367 bp (GRCm38)
  • A to T, chromosome 9 at 72,856,825 bp (GRCm38)
  • T to A, chromosome 10 at 97,612,968 bp (GRCm38)
  • C to T, chromosome 10 at 110,782,224 bp (GRCm38)
  • T to C, chromosome 11 at 67,670,620 bp (GRCm38)
  • T to C, chromosome 11 at 75,496,514 bp (GRCm38)
  • A to T, chromosome 11 at 86,561,265 bp (GRCm38)
  • C to A, chromosome 11 at 94,632,133 bp (GRCm38)
  • T to C, chromosome 11 at 95,300,102 bp (GRCm38)
  • A to T, chromosome 11 at 95,574,221 bp (GRCm38)
  • T to G, chromosome 11 at 96,353,513 bp (GRCm38)
  • T to A, chromosome 11 at 98,019,708 bp (GRCm38)
  • C to A, chromosome 11 at 115,392,711 bp (GRCm38)
  • T to A, chromosome 11 at 121,513,154 bp (GRCm38)
  • C to T, chromosome 12 at 32,192,214 bp (GRCm38)
  • A to G, chromosome 12 at 76,627,002 bp (GRCm38)
  • A to T, chromosome 13 at 30,757,501 bp (GRCm38)
  • G to A, chromosome 13 at 63,171,048 bp (GRCm38)
  • A to C, chromosome 13 at 94,471,728 bp (GRCm38)
  • A to T, chromosome 14 at 14,329,059 bp (GRCm38)
  • C to T, chromosome 14 at 47,413,749 bp (GRCm38)
  • A to G, chromosome 14 at 56,761,848 bp (GRCm38)
  • C to A, chromosome 14 at 69,797,254 bp (GRCm38)
  • A to G, chromosome 15 at 58,872,759 bp (GRCm38)
  • A to C, chromosome 15 at 74,548,626 bp (GRCm38)
  • T to C, chromosome 16 at 20,735,822 bp (GRCm38)
  • A to G, chromosome 16 at 60,604,875 bp (GRCm38)
  • A to G, chromosome 16 at 91,056,158 bp (GRCm38)
  • G to T, chromosome 16 at 95,389,948 bp (GRCm38)
  • A to G, chromosome 17 at 25,124,572 bp (GRCm38)
  • A to G, chromosome 17 at 31,850,753 bp (GRCm38)
  • T to C, chromosome 18 at 34,462,199 bp (GRCm38)
  • A to T, chromosome 18 at 46,560,981 bp (GRCm38)
  • T to C, chromosome 19 at 48,796,372 bp (GRCm38)
  • A to T, chromosome X at 145,461,749 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9166 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069023-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.