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Strain Name:
C57BL/6J-MtgxR9186Btlr/Mmmh
Stock Number:
069034-MU
Citation ID:
RRID:MMRRC_069034-MU
Other Names:
R9186 (G1)
Major Collection:

Strain Information

Hps5
Name: HPS5, biogenesis of lysosomal organelles complex 2 subunit 2
Synonyms: ru-2, ruby eye 2, ru2, Hermansky-Pudlak syndrome 5
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 246694
Homologene: 35333
Utrn
Name: utrophin
Synonyms: G-utrophin, Dmdl, DRP
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 22288
VEGA: 10
Homologene: 21398
Zdhhc17
Name: zinc finger, DHHC domain containing 17
Synonyms: A230053P19Rik, D130071N24Rik, Hip14
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 320150
VEGA: 10
Homologene: 56324
Colgalt2
Name: collagen beta(1-O)galactosyltransferase 2
Synonyms: Glt25d2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 269132
Homologene: 22865
Trpc2
Name: transient receptor potential cation channel, subfamily C, member 2
Synonyms: TRPC2b, TRPC2a, mTrp2, trp2, Trrp2, 3010009O07Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 22064
Homologene: 135989
Lgi3
Name: leucine-rich repeat LGI family, member 3
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 213469
VEGA: 14
Homologene: 16406
Prtg
Name: protogenin
Synonyms: A230098A12Rik, Igdcc5
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235472
Homologene: 54453
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • G to A, chromosome 1 at 36,779,751 bp (GRCm38)
  • C to T, chromosome 1 at 133,292,433 bp (GRCm38)
  • C to A, chromosome 1 at 152,508,652 bp (GRCm38)
  • A to G, chromosome 1 at 164,193,901 bp (GRCm38)
  • T to C, chromosome 2 at 104,471,094 bp (GRCm38)
  • C to A, chromosome 2 at 112,042,750 bp (GRCm38)
  • T to A, chromosome 2 at 140,148,872 bp (GRCm38)
  • A to G, chromosome 2 at 181,234,664 bp (GRCm38)
  • T to C, chromosome 3 at 65,958,005 bp (GRCm38)
  • T to C, chromosome 3 at 108,044,733 bp (GRCm38)
  • A to T, chromosome 3 at 152,220,516 bp (GRCm38)
  • A to G, chromosome 4 at 118,385,091 bp (GRCm38)
  • C to A, chromosome 4 at 123,322,668 bp (GRCm38)
  • T to A, chromosome 5 at 22,547,946 bp (GRCm38)
  • C to A, chromosome 5 at 87,843,135 bp (GRCm38)
  • G to A, chromosome 5 at 110,252,744 bp (GRCm38)
  • C to A, chromosome 5 at 137,393,548 bp (GRCm38)
  • T to C, chromosome 5 at 142,787,733 bp (GRCm38)
  • C to A, chromosome 6 at 8,537,826 bp (GRCm38)
  • A to T, chromosome 6 at 24,450,767 bp (GRCm38)
  • T to C, chromosome 6 at 24,664,616 bp (GRCm38)
  • C to T, chromosome 6 at 113,710,164 bp (GRCm38)
  • T to A, chromosome 6 at 122,075,289 bp (GRCm38)
  • T to C, chromosome 7 at 4,629,726 bp (GRCm38)
  • T to A, chromosome 7 at 25,527,788 bp (GRCm38)
  • T to C, chromosome 7 at 46,788,946 bp (GRCm38)
  • T to C, chromosome 7 at 102,088,285 bp (GRCm38)
  • A to T, chromosome 8 at 35,815,475 bp (GRCm38)
  • C to T, chromosome 8 at 46,191,628 bp (GRCm38)
  • T to C, chromosome 8 at 71,596,189 bp (GRCm38)
  • G to T, chromosome 8 at 83,290,643 bp (GRCm38)
  • G to A, chromosome 8 at 91,101,378 bp (GRCm38)
  • C to A, chromosome 9 at 57,257,189 bp (GRCm38)
  • A to T, chromosome 9 at 57,794,934 bp (GRCm38)
  • T to C, chromosome 9 at 72,856,877 bp (GRCm38)
  • T to A, chromosome 9 at 96,133,416 bp (GRCm38)
  • T to C, chromosome 10 at 12,614,574 bp (GRCm38)
  • C to A, chromosome 10 at 41,528,699 bp (GRCm38)
  • G to A, chromosome 10 at 60,307,527 bp (GRCm38)
  • T to C, chromosome 10 at 110,944,420 bp (GRCm38)
  • T to C, chromosome 10 at 121,275,734 bp (GRCm38)
  • A to T, chromosome 11 at 42,487,373 bp (GRCm38)
  • C to T, chromosome 11 at 77,859,021 bp (GRCm38)
  • T to A, chromosome 12 at 118,190,897 bp (GRCm38)
  • T to A, chromosome 13 at 12,421,346 bp (GRCm38)
  • A to G, chromosome 13 at 54,578,807 bp (GRCm38)
  • T to A, chromosome 13 at 94,208,174 bp (GRCm38)
  • A to C, chromosome 13 at 97,189,328 bp (GRCm38)
  • T to C, chromosome 13 at 100,299,882 bp (GRCm38)
  • A to T, chromosome 14 at 21,748,495 bp (GRCm38)
  • A to G, chromosome 14 at 70,534,753 bp (GRCm38)
  • G to A, chromosome 14 at 123,857,558 bp (GRCm38)
  • T to A, chromosome 15 at 79,727,536 bp (GRCm38)
  • T to C, chromosome 16 at 18,251,169 bp (GRCm38)
  • C to A, chromosome 16 at 88,647,721 bp (GRCm38)
  • T to C, chromosome 17 at 24,604,888 bp (GRCm38)
  • C to T, chromosome 17 at 35,144,691 bp (GRCm38)
  • G to T, chromosome 17 at 46,245,707 bp (GRCm38)
  • A to T, chromosome 17 at 65,593,512 bp (GRCm38)
  • T to C, chromosome 18 at 36,634,330 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9186 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069034-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.