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Strain Name:
C57BL/6J-MtgxR9203Btlr/Mmmh
Stock Number:
069043-MU
Citation ID:
RRID:MMRRC_069043-MU
Other Names:
R9203 (G1)
Major Collection:

Strain Information

Eno1
Name: enolase 1, alpha non-neuron
Synonyms: 2-phospho-D-glycerate hydrolase, alpha-enolase, Eno-1, MBP-1, c-Myc promoter binding protein
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 13806
HGNC: HGNC:3350
Homologene: 134343
Pigv
Name: phosphatidylinositol glycan anchor biosynthesis, class V
Synonyms: D430024F16Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230801
Homologene: 5354
Wdr70
Name: WD repeat domain 70
Synonyms: 4833422F06Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 545085
VEGA: 15
Homologene: 9972
Pi4ka
Name: phosphatidylinositol 4-kinase alpha
Synonyms: Pik4ca
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 224020
HGNC: HGNC:8983
Homologene: 11171
Anapc1
Name: anaphase promoting complex subunit 1
Synonyms: tsg24, Apc1, 2610021O03Rik, Mcpr
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 17222
Homologene: 7414
Cc2d2a
Name: coiled-coil and C2 domain containing 2A
Synonyms: 5730509K17Rik, b2b1035Clo
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231214
Homologene: 18159
Grm4
Name: glutamate receptor, metabotropic 4
Synonyms: mGluR4, Gprc1d
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 268934
HGNC: HGNC:4596
Homologene: 20230
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • T to A, chromosome 1 at 180,333,439 bp (GRCm38)
  • G to T, chromosome 2 at 29,194,986 bp (GRCm38)
  • T to C, chromosome 2 at 74,746,400 bp (GRCm38)
  • C to T, chromosome 2 at 121,457,682 bp (GRCm38)
  • T to C, chromosome 2 at 128,623,502 bp (GRCm38)
  • C to T, chromosome 2 at 164,354,897 bp (GRCm38)
  • T to C, chromosome 2 at 178,355,113 bp (GRCm38)
  • C to T, chromosome 2 at 179,780,403 bp (GRCm38)
  • C to T, chromosome 3 at 125,724,736 bp (GRCm38)
  • A to G, chromosome 4 at 114,602,925 bp (GRCm38)
  • A to T, chromosome 4 at 133,665,679 bp (GRCm38)
  • T to A, chromosome 4 at 143,897,076 bp (GRCm38)
  • C to T, chromosome 4 at 150,248,082 bp (GRCm38)
  • C to T, chromosome 5 at 14,678,514 bp (GRCm38)
  • G to T, chromosome 5 at 43,733,837 bp (GRCm38)
  • C to A, chromosome 5 at 64,979,957 bp (GRCm38)
  • A to T, chromosome 6 at 56,779,002 bp (GRCm38)
  • T to A, chromosome 6 at 140,007,755 bp (GRCm38)
  • T to A, chromosome 7 at 4,571,971 bp (GRCm38)
  • T to C, chromosome 7 at 18,478,457 bp (GRCm38)
  • A to G, chromosome 7 at 43,463,233 bp (GRCm38)
  • A to C, chromosome 7 at 79,704,724 bp (GRCm38)
  • T to C, chromosome 7 at 85,005,742 bp (GRCm38)
  • A to T, chromosome 7 at 103,220,655 bp (GRCm38)
  • C to T, chromosome 8 at 80,704,629 bp (GRCm38)
  • T to C, chromosome 8 at 105,986,253 bp (GRCm38)
  • T to C, chromosome 8 at 106,874,220 bp (GRCm38)
  • A to G, chromosome 9 at 18,551,688 bp (GRCm38)
  • A to T, chromosome 10 at 83,641,015 bp (GRCm38)
  • T to G, chromosome 10 at 93,045,128 bp (GRCm38)
  • A to G, chromosome 11 at 34,731,539 bp (GRCm38)
  • A to T, chromosome 11 at 51,048,334 bp (GRCm38)
  • A to G, chromosome 11 at 65,855,287 bp (GRCm38)
  • T to C, chromosome 11 at 73,191,737 bp (GRCm38)
  • A to G, chromosome 11 at 79,502,225 bp (GRCm38)
  • C to G, chromosome 11 at 100,188,908 bp (GRCm38)
  • T to C, chromosome 13 at 14,316,658 bp (GRCm38)
  • T to A, chromosome 13 at 18,129,030 bp (GRCm38)
  • T to C, chromosome 13 at 21,786,887 bp (GRCm38)
  • C to A, chromosome 13 at 24,836,933 bp (GRCm38)
  • G to T, chromosome 13 at 96,088,237 bp (GRCm38)
  • G to T, chromosome 14 at 26,961,013 bp (GRCm38)
  • T to C, chromosome 14 at 30,051,712 bp (GRCm38)
  • T to C, chromosome 14 at 50,819,393 bp (GRCm38)
  • A to T, chromosome 14 at 52,008,948 bp (GRCm38)
  • A to T, chromosome 14 at 119,057,563 bp (GRCm38)
  • G to A, chromosome 15 at 7,873,203 bp (GRCm38)
  • A to G, chromosome 15 at 31,297,882 bp (GRCm38)
  • G to T, chromosome 15 at 86,033,085 bp (GRCm38)
  • G to T, chromosome 16 at 17,282,301 bp (GRCm38)
  • T to A, chromosome 16 at 64,478,267 bp (GRCm38)
  • T to C, chromosome 17 at 27,435,006 bp (GRCm38)
  • A to G, chromosome 17 at 80,227,808 bp (GRCm38)
  • G to A, chromosome 18 at 12,462,812 bp (GRCm38)
  • G to A, chromosome 18 at 24,014,269 bp (GRCm38)
  • T to C, chromosome 18 at 63,157,231 bp (GRCm38)
  • T to C, chromosome 19 at 45,553,652 bp (GRCm38)
  • A to G, chromosome 19 at 50,262,295 bp (GRCm38)
  • A to G, chromosome Y at 940,981 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9203 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069043-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.