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Strain Name:
C57BL/6J-MtgxR9254Btlr/Mmmh
Stock Number:
069077-MU
Citation ID:
RRID:MMRRC_069077-MU
Other Names:
R9254 (G1)
Major Collection:

Strain Information

Chd7
Name: chromodomain helicase DNA binding protein 7
Synonyms: GENA 60, GENA 47, Gena 52, Cyn, A730019I05Rik, WBE1, Whi, Todo, Obt, Mt, Lda, Flo, Edy, Dz, Cycn
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 320790
Homologene: 19067
Dspp
Name: dentin sialophosphoprotein
Synonyms: Dmp3, Dpp, Dsp
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 666279
HGNC: HGNC:3054
Nos1
Name: nitric oxide synthase 1, neuronal
Synonyms: nNOS, bNOS, Nos-1, NO, 2310005C01Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18125
HGNC: HGNC:7872
Homologene: 37327
B230219D22Rik
Name: RIKEN cDNA B230219D22 gene
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 78521
VEGA: 13
Homologene: 17572
Agap1
Name: ArfGAP with GTPase domain, ankyrin repeat and PH domain 1
Synonyms: Ggap1, Centg2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 347722
Homologene: 56689
Lrp2
Name: low density lipoprotein receptor-related protein 2
Synonyms: Megalin, Gp330, D230004K18Rik, b2b1625.2Clo
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14725
HGNC: HGNC:6694
Homologene: 20952
Tkfc
Name: triokinase, FMN cyclase
Synonyms: Dak
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 225913
VEGA: 19
Homologene: 56710
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • T to C, chromosome 1 at 17,624,956 bp (GRCm38)
  • A to G, chromosome 1 at 84,695,472 bp (GRCm38)
  • T to A, chromosome 1 at 89,726,019 bp (GRCm38)
  • C to T, chromosome 1 at 167,308,563 bp (GRCm38)
  • A to G, chromosome 1 at 180,694,693 bp (GRCm38)
  • C to T, chromosome 1 at 194,810,166 bp (GRCm38)
  • A to G, chromosome 2 at 21,368,231 bp (GRCm38)
  • T to C, chromosome 2 at 69,503,547 bp (GRCm38)
  • T to A, chromosome 2 at 87,738,028 bp (GRCm38)
  • A to G, chromosome 2 at 103,711,235 bp (GRCm38)
  • C to T, chromosome 2 at 158,200,963 bp (GRCm38)
  • A to G, chromosome 4 at 8,752,210 bp (GRCm38)
  • T to A, chromosome 4 at 96,217,503 bp (GRCm38)
  • A to G, chromosome 4 at 128,197,319 bp (GRCm38)
  • A to T, chromosome 4 at 129,637,107 bp (GRCm38)
  • T to C, chromosome 5 at 35,044,789 bp (GRCm38)
  • T to C, chromosome 5 at 41,821,880 bp (GRCm38)
  • A to T, chromosome 5 at 104,174,894 bp (GRCm38)
  • T to A, chromosome 5 at 105,608,490 bp (GRCm38)
  • G to C, chromosome 5 at 117,879,337 bp (GRCm38)
  • G to A, chromosome 5 at 121,747,446 bp (GRCm38)
  • T to C, chromosome 5 at 122,473,252 bp (GRCm38)
  • A to G, chromosome 5 at 138,183,690 bp (GRCm38)
  • G to T, chromosome 6 at 48,487,994 bp (GRCm38)
  • T to A, chromosome 6 at 57,955,751 bp (GRCm38)
  • A to G, chromosome 6 at 86,937,067 bp (GRCm38)
  • T to A, chromosome 6 at 115,576,903 bp (GRCm38)
  • G to A, chromosome 7 at 16,886,088 bp (GRCm38)
  • A to G, chromosome 7 at 45,947,692 bp (GRCm38)
  • A to G, chromosome 7 at 84,947,442 bp (GRCm38)
  • G to T, chromosome 7 at 120,207,979 bp (GRCm38)
  • T to C, chromosome 7 at 125,870,676 bp (GRCm38)
  • T to C, chromosome 8 at 45,289,030 bp (GRCm38)
  • T to A, chromosome 9 at 18,646,171 bp (GRCm38)
  • T to A, chromosome 9 at 19,845,916 bp (GRCm38)
  • C to T, chromosome 9 at 37,919,151 bp (GRCm38)
  • A to G, chromosome 9 at 39,686,818 bp (GRCm38)
  • C to T, chromosome 9 at 108,294,509 bp (GRCm38)
  • A to T, chromosome 10 at 25,993,756 bp (GRCm38)
  • A to G, chromosome 10 at 27,422,689 bp (GRCm38)
  • T to G, chromosome 10 at 41,612,948 bp (GRCm38)
  • A to T, chromosome 10 at 85,943,207 bp (GRCm38)
  • T to A, chromosome 10 at 93,852,773 bp (GRCm38)
  • G to T, chromosome 10 at 97,507,781 bp (GRCm38)
  • G to T, chromosome 10 at 119,945,056 bp (GRCm38)
  • T to A, chromosome 10 at 128,816,874 bp (GRCm38)
  • A to G, chromosome 10 at 130,180,736 bp (GRCm38)
  • T to C, chromosome 11 at 115,578,598 bp (GRCm38)
  • T to C, chromosome 11 at 116,458,108 bp (GRCm38)
  • G to C, chromosome 12 at 55,722,798 bp (GRCm38)
  • T to C, chromosome 12 at 78,627,262 bp (GRCm38)
  • T to C, chromosome 12 at 114,773,043 bp (GRCm38)
  • C to T, chromosome 13 at 11,883,116 bp (GRCm38)
  • T to A, chromosome 13 at 27,059,520 bp (GRCm38)
  • C to A, chromosome 13 at 55,699,470 bp (GRCm38)
  • T to C, chromosome 13 at 59,788,474 bp (GRCm38)
  • T to C, chromosome 14 at 20,531,806 bp (GRCm38)
  • T to G, chromosome 14 at 53,337,396 bp (GRCm38)
  • A to G, chromosome 15 at 76,408,015 bp (GRCm38)
  • C to T, chromosome 15 at 90,969,827 bp (GRCm38)
  • T to C, chromosome 16 at 20,333,687 bp (GRCm38)
  • G to A, chromosome 16 at 32,470,076 bp (GRCm38)
  • A to T, chromosome 16 at 56,705,687 bp (GRCm38)
  • T to C, chromosome 17 at 20,275,804 bp (GRCm38)
  • A to G, chromosome 17 at 25,961,882 bp (GRCm38)
  • T to C, chromosome 17 at 33,641,467 bp (GRCm38)
  • T to C, chromosome 18 at 7,265,089 bp (GRCm38)
  • C to T, chromosome 18 at 14,813,374 bp (GRCm38)
  • T to C, chromosome 18 at 36,644,627 bp (GRCm38)
  • T to C, chromosome 18 at 49,891,500 bp (GRCm38)
  • A to G, chromosome 19 at 5,053,221 bp (GRCm38)
  • T to A, chromosome 19 at 10,597,348 bp (GRCm38)
  • T to C, chromosome 19 at 42,063,258 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9254 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069077-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.