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Strain Name:
C57BL/6J-MtgxR9297Btlr/Mmmh
Stock Number:
069114-MU
Citation ID:
RRID:MMRRC_069114-MU
Other Names:
R9297 (G1)
Major Collection:

Strain Information

Msx1
Name: msh homeobox 1
Synonyms: Hox7, Hox7.1, muscle-segment homeobox, msh, Hox-7
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 17701
HGNC: HGNC:7391
Homologene: 1836
Adamts20
Name: ADAM metallopeptidase with thrombospondin type 1 motif 20
Synonyms: ADAMTS-20, bt
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223838
Homologene: 11808
Crlf3
Name: cytokine receptor-like factor 3
Synonyms: cytor4, Creme9
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 54394
Homologene: 9327
Phf20
Name: PHD finger protein 20
Synonyms: 6820402O20Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228829
Homologene: 9507
Zfp42
Name: zinc finger protein 42
Synonyms: Rex-1, Zfp-42, Rex1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 22702
Homologene: 7601
Usp48
Name: ubiquitin specific peptidase 48
Synonyms: D330022K21Rik, 2810449C13Rik, Usp31
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 170707
Homologene: 12988
Map4
Name: microtubule-associated protein 4
Synonyms: MAP 4, Mtap4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17758
HGNC: HGNC:6862
Homologene: 1780
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • A to T, chromosome 1 at 10,484,048 bp (GRCm38)
  • T to A, chromosome 1 at 20,222,894 bp (GRCm38)
  • A to T, chromosome 1 at 59,544,670 bp (GRCm38)
  • T to C, chromosome 1 at 140,425,195 bp (GRCm38)
  • G to A, chromosome 1 at 152,524,703 bp (GRCm38)
  • G to T, chromosome 1 at 153,252,000 bp (GRCm38)
  • C to T, chromosome 1 at 178,715,809 bp (GRCm38)
  • A to G, chromosome 2 at 77,011,684 bp (GRCm38)
  • A to G, chromosome 2 at 86,332,844 bp (GRCm38)
  • A to G, chromosome 2 at 94,004,737 bp (GRCm38)
  • G to A, chromosome 2 at 156,273,770 bp (GRCm38)
  • A to G, chromosome 2 at 161,575,778 bp (GRCm38)
  • A to G, chromosome 3 at 86,373,566 bp (GRCm38)
  • T to C, chromosome 4 at 65,542,723 bp (GRCm38)
  • A to C, chromosome 4 at 84,555,899 bp (GRCm38)
  • A to T, chromosome 4 at 112,811,520 bp (GRCm38)
  • T to C, chromosome 4 at 115,521,178 bp (GRCm38)
  • G to A, chromosome 4 at 137,613,685 bp (GRCm38)
  • G to A, chromosome 5 at 37,824,412 bp (GRCm38)
  • A to G, chromosome 6 at 23,496,888 bp (GRCm38)
  • A to T, chromosome 6 at 40,514,975 bp (GRCm38)
  • T to A, chromosome 6 at 54,295,855 bp (GRCm38)
  • A to G, chromosome 6 at 131,689,324 bp (GRCm38)
  • C to A, chromosome 6 at 148,801,578 bp (GRCm38)
  • C to T, chromosome 7 at 25,308,832 bp (GRCm38)
  • G to T, chromosome 7 at 25,331,086 bp (GRCm38)
  • T to C, chromosome 7 at 29,191,660 bp (GRCm38)
  • A to T, chromosome 7 at 46,376,932 bp (GRCm38)
  • T to A, chromosome 7 at 103,329,376 bp (GRCm38)
  • T to C, chromosome 7 at 105,035,623 bp (GRCm38)
  • T to C, chromosome 7 at 115,662,322 bp (GRCm38)
  • A to G, chromosome 7 at 141,749,022 bp (GRCm38)
  • A to G, chromosome 8 at 3,716,500 bp (GRCm38)
  • C to T, chromosome 8 at 40,938,231 bp (GRCm38)
  • T to C, chromosome 8 at 43,295,735 bp (GRCm38)
  • T to C, chromosome 8 at 57,542,521 bp (GRCm38)
  • A to G, chromosome 8 at 94,911,492 bp (GRCm38)
  • A to C, chromosome 8 at 105,379,275 bp (GRCm38)
  • A to T, chromosome 9 at 15,997,700 bp (GRCm38)
  • C to A, chromosome 9 at 58,086,593 bp (GRCm38)
  • T to A, chromosome 9 at 70,820,454 bp (GRCm38)
  • T to C, chromosome 9 at 110,053,412 bp (GRCm38)
  • T to G, chromosome 10 at 41,423,085 bp (GRCm38)
  • C to A, chromosome 10 at 41,630,114 bp (GRCm38)
  • T to A, chromosome 10 at 45,652,673 bp (GRCm38)
  • T to C, chromosome 11 at 5,040,157 bp (GRCm38)
  • A to G, chromosome 11 at 53,134,146 bp (GRCm38)
  • A to T, chromosome 11 at 59,007,533 bp (GRCm38)
  • A to G, chromosome 11 at 60,495,073 bp (GRCm38)
  • A to G, chromosome 11 at 75,416,425 bp (GRCm38)
  • A to G, chromosome 11 at 80,059,205 bp (GRCm38)
  • A to T, chromosome 11 at 94,650,788 bp (GRCm38)
  • A to G, chromosome 11 at 97,672,488 bp (GRCm38)
  • A to G, chromosome 11 at 100,103,445 bp (GRCm38)
  • T to C, chromosome 12 at 87,458,904 bp (GRCm38)
  • T to C, chromosome 13 at 13,476,312 bp (GRCm38)
  • T to A, chromosome 13 at 21,484,031 bp (GRCm38)
  • A to T, chromosome 13 at 96,878,577 bp (GRCm38)
  • A to G, chromosome 13 at 100,696,412 bp (GRCm38)
  • T to A, chromosome 14 at 30,866,682 bp (GRCm38)
  • T to A, chromosome 14 at 37,062,036 bp (GRCm38)
  • T to C, chromosome 14 at 52,009,037 bp (GRCm38)
  • A to C, chromosome 15 at 28,203,908 bp (GRCm38)
  • A to G, chromosome 15 at 42,438,355 bp (GRCm38)
  • G to T, chromosome 15 at 78,410,442 bp (GRCm38)
  • C to A, chromosome 15 at 79,527,074 bp (GRCm38)
  • A to G, chromosome 15 at 94,403,440 bp (GRCm38)
  • A to T, chromosome 15 at 98,593,585 bp (GRCm38)
  • A to G, chromosome 16 at 45,167,008 bp (GRCm38)
  • T to C, chromosome 16 at 85,802,646 bp (GRCm38)
  • A to G, chromosome 17 at 28,217,740 bp (GRCm38)
  • A to T, chromosome 17 at 56,458,257 bp (GRCm38)
  • G to A, chromosome 18 at 37,889,775 bp (GRCm38)
  • C to T, chromosome 18 at 62,922,660 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9297 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069114-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.