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Strain Name:
C57BL/6J-MtgxR9300Btlr/Mmmh
Stock Number:
069116-MU
Citation ID:
RRID:MMRRC_069116-MU
Other Names:
R9300 (G1)
Major Collection:

Strain Information

Shh
Name: sonic hedgehog
Synonyms: Hhg1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 20423
Homologene: 30961
Itm2b
Name: integral membrane protein 2B
Synonyms: D14Sel6, Bri2, Bricd2b
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 16432
VEGA: 14
HGNC: HGNC:6174
Homologene: 7388
Slc6a5
Name: solute carrier family 6 (neurotransmitter transporter, glycine), member 5
Synonyms: Glyt2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 104245
Homologene: 37901
Magi1
Name: membrane associated guanylate kinase, WW and PDZ domain containing 1
Synonyms: WWP3, Gukmi1, AIP3, BAP1, Baiap1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 14924
HGNC: HGNC:946
Homologene: 31257
Cadm1
Name: cell adhesion molecule 1
Synonyms: 3100001I08Rik, 2900073G06Rik, RA175N, RA175C, RA175B, RA175A, Necl2, SgIGSF, Tslc1, SynCam, Igsf4, Igsf4a
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 54725
HGNC: HGNC:5951
Homologene: 8641
Senp6
Name: SUMO/sentrin specific peptidase 6
Synonyms: E130319N12Rik, 2810017C20Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 215351
Homologene: 9196
Kif16b
Name: kinesin family member 16B
Synonyms: N-3 kinesin, 8430434E15Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16558
Homologene: 135708
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • A to G, chromosome 1 at 44,117,202 bp (GRCm38)
  • G to T, chromosome 1 at 97,066,084 bp (GRCm38)
  • G to A, chromosome 1 at 125,981,686 bp (GRCm38)
  • T to C, chromosome 1 at 133,914,519 bp (GRCm38)
  • G to A, chromosome 1 at 155,705,210 bp (GRCm38)
  • T to C, chromosome 1 at 156,441,353 bp (GRCm38)
  • A to G, chromosome 1 at 172,239,831 bp (GRCm38)
  • C to T, chromosome 2 at 52,111,139 bp (GRCm38)
  • T to A, chromosome 2 at 66,504,892 bp (GRCm38)
  • C to T, chromosome 2 at 72,371,913 bp (GRCm38)
  • A to T, chromosome 2 at 112,860,350 bp (GRCm38)
  • T to C, chromosome 2 at 121,302,965 bp (GRCm38)
  • G to T, chromosome 2 at 142,699,287 bp (GRCm38)
  • A to G, chromosome 2 at 167,055,940 bp (GRCm38)
  • T to C, chromosome 3 at 98,427,663 bp (GRCm38)
  • T to C, chromosome 3 at 106,202,558 bp (GRCm38)
  • A to G, chromosome 4 at 73,741,156 bp (GRCm38)
  • G to A, chromosome 4 at 87,774,047 bp (GRCm38)
  • G to A, chromosome 5 at 8,825,614 bp (GRCm38)
  • A to G, chromosome 5 at 28,458,463 bp (GRCm38)
  • T to C, chromosome 5 at 38,527,912 bp (GRCm38)
  • T to G, chromosome 5 at 43,609,099 bp (GRCm38)
  • A to T, chromosome 5 at 66,313,334 bp (GRCm38)
  • G to A, chromosome 5 at 120,664,107 bp (GRCm38)
  • G to A, chromosome 5 at 121,348,889 bp (GRCm38)
  • A to T, chromosome 5 at 124,712,248 bp (GRCm38)
  • A to G, chromosome 5 at 134,498,088 bp (GRCm38)
  • A to G, chromosome 5 at 134,729,366 bp (GRCm38)
  • A to T, chromosome 5 at 137,470,257 bp (GRCm38)
  • G to C, chromosome 5 at 145,720,096 bp (GRCm38)
  • G to A, chromosome 5 at 145,862,354 bp (GRCm38)
  • G to A, chromosome 6 at 57,137,887 bp (GRCm38)
  • G to T, chromosome 6 at 92,887,390 bp (GRCm38)
  • C to A, chromosome 6 at 93,747,228 bp (GRCm38)
  • A to G, chromosome 6 at 123,816,071 bp (GRCm38)
  • G to T, chromosome 7 at 21,011,713 bp (GRCm38)
  • A to T, chromosome 7 at 49,951,427 bp (GRCm38)
  • G to A, chromosome 7 at 50,062,620 bp (GRCm38)
  • A to T, chromosome 7 at 106,818,904 bp (GRCm38)
  • G to A, chromosome 9 at 14,464,040 bp (GRCm38)
  • A to T, chromosome 9 at 15,257,289 bp (GRCm38)
  • G to C, chromosome 9 at 21,035,550 bp (GRCm38)
  • A to T, chromosome 9 at 26,977,632 bp (GRCm38)
  • A to C, chromosome 9 at 37,730,282 bp (GRCm38)
  • A to T, chromosome 9 at 47,797,523 bp (GRCm38)
  • A to G, chromosome 9 at 55,488,868 bp (GRCm38)
  • A to G, chromosome 9 at 57,107,460 bp (GRCm38)
  • A to G, chromosome 9 at 80,142,151 bp (GRCm38)
  • A to G, chromosome 9 at 106,847,843 bp (GRCm38)
  • A to G, chromosome 9 at 124,423,977 bp (GRCm38)
  • A to T, chromosome 10 at 58,507,626 bp (GRCm38)
  • A to T, chromosome 10 at 69,871,042 bp (GRCm38)
  • T to A, chromosome 10 at 90,577,104 bp (GRCm38)
  • A to G, chromosome 11 at 5,569,979 bp (GRCm38)
  • A to G, chromosome 11 at 35,815,661 bp (GRCm38)
  • T to C, chromosome 11 at 50,163,887 bp (GRCm38)
  • T to A, chromosome 11 at 62,877,283 bp (GRCm38)
  • T to C, chromosome 11 at 72,301,691 bp (GRCm38)
  • G to A, chromosome 11 at 80,817,606 bp (GRCm38)
  • A to T, chromosome 11 at 85,557,358 bp (GRCm38)
  • A to G, chromosome 11 at 113,825,030 bp (GRCm38)
  • T to C, chromosome 12 at 4,775,442 bp (GRCm38)
  • T to C, chromosome 12 at 73,940,528 bp (GRCm38)
  • T to A, chromosome 13 at 21,393,497 bp (GRCm38)
  • C to A, chromosome 13 at 21,495,367 bp (GRCm38)
  • T to G, chromosome 13 at 111,686,356 bp (GRCm38)
  • A to T, chromosome 14 at 4,777,288 bp (GRCm38)
  • C to T, chromosome 14 at 26,665,776 bp (GRCm38)
  • G to A, chromosome 14 at 55,704,846 bp (GRCm38)
  • T to C, chromosome 14 at 56,460,038 bp (GRCm38)
  • A to T, chromosome 14 at 73,366,456 bp (GRCm38)
  • T to C, chromosome 15 at 39,146,707 bp (GRCm38)
  • G to T, chromosome 15 at 76,614,559 bp (GRCm38)
  • C to A, chromosome 15 at 76,646,341 bp (GRCm38)
  • T to C, chromosome 15 at 93,500,868 bp (GRCm38)
  • T to G, chromosome 15 at 97,713,073 bp (GRCm38)
  • C to T, chromosome 15 at 99,806,439 bp (GRCm38)
  • T to C, chromosome 16 at 17,574,535 bp (GRCm38)
  • C to A, chromosome 17 at 5,242,999 bp (GRCm38)
  • T to A, chromosome 17 at 21,286,683 bp (GRCm38)
  • T to A, chromosome 17 at 23,632,754 bp (GRCm38)
  • C to T, chromosome 17 at 37,700,924 bp (GRCm38)
  • T to A, chromosome 17 at 73,239,924 bp (GRCm38)
  • C to T, chromosome 18 at 36,682,221 bp (GRCm38)
  • T to A, chromosome 18 at 53,719,297 bp (GRCm38)
  • G to T, chromosome 19 at 4,871,628 bp (GRCm38)
  • G to A, chromosome 19 at 8,884,186 bp (GRCm38)
  • A to C, chromosome 19 at 22,978,381 bp (GRCm38)
  • T to C, chromosome 19 at 38,396,185 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9300 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069116-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.