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Strain Name:
C57BL/6J-MtgxR9324Btlr/Mmmh
Stock Number:
069136-MU
Citation ID:
RRID:MMRRC_069136-MU
Other Names:
R9324 (G1)
Major Collection:

Strain Information

Slc26a5
Name: solute carrier family 26, member 5
Synonyms: Pres, prestin
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 80979
HGNC: HGNC:9359
Homologene: 69472
Ntrk1
Name: neurotrophic tyrosine kinase, receptor, type 1
Synonyms: TrkA, Tkr
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18211
HGNC: HGNC:8031
Homologene: 1898
Arpp21
Name: cyclic AMP-regulated phosphoprotein, 21
Synonyms: ARPP-21, Tarpp, D9Bwg1012e, 0710001E13Rik, R3hdm3
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 74100
Homologene: 32306
Ppcdc
Name: phosphopantothenoylcysteine decarboxylase
Synonyms: 8430432M10Rik, 1810057I13Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 66812
VEGA: 9
Homologene: 11049
Rngtt
Name: RNA guanylyltransferase and 5'-phosphatase
Synonyms: mouse capping enzyme
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 24018
Homologene: 37851
Ggnbp2
Name: gametogenetin binding protein 2
Synonyms: DIF-3, Zfp403, D330017P12Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217039
Homologene: 11750
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • T to C, chromosome 1 at 133,319,356 bp (GRCm38)
  • T to C, chromosome 2 at 62,645,606 bp (GRCm38)
  • T to C, chromosome 2 at 69,795,569 bp (GRCm38)
  • C to T, chromosome 2 at 88,487,443 bp (GRCm38)
  • T to C, chromosome 2 at 146,460,725 bp (GRCm38)
  • T to C, chromosome 2 at 174,705,320 bp (GRCm38)
  • T to C, chromosome 3 at 85,730,746 bp (GRCm38)
  • C to T, chromosome 3 at 87,791,438 bp (GRCm38)
  • C to A, chromosome 4 at 3,172,678 bp (GRCm38)
  • C to G, chromosome 4 at 25,008,432 bp (GRCm38)
  • T to A, chromosome 4 at 33,320,613 bp (GRCm38)
  • A to G, chromosome 4 at 41,730,138 bp (GRCm38)
  • A to T, chromosome 4 at 107,247,716 bp (GRCm38)
  • T to A, chromosome 4 at 146,535,427 bp (GRCm38)
  • T to C, chromosome 4 at 148,608,028 bp (GRCm38)
  • G to T, chromosome 5 at 21,813,336 bp (GRCm38)
  • T to C, chromosome 5 at 39,614,802 bp (GRCm38)
  • C to T, chromosome 5 at 136,700,933 bp (GRCm38)
  • T to C, chromosome 5 at 144,880,653 bp (GRCm38)
  • A to G, chromosome 5 at 147,376,980 bp (GRCm38)
  • T to C, chromosome 6 at 113,638,643 bp (GRCm38)
  • C to A, chromosome 6 at 135,225,795 bp (GRCm38)
  • G to A, chromosome 7 at 5,359,528 bp (GRCm38)
  • T to C, chromosome 7 at 9,027,685 bp (GRCm38)
  • C to G, chromosome 7 at 25,761,557 bp (GRCm38)
  • A to G, chromosome 7 at 27,529,409 bp (GRCm38)
  • A to C, chromosome 7 at 30,715,782 bp (GRCm38)
  • G to T, chromosome 7 at 42,267,195 bp (GRCm38)
  • A to T, chromosome 7 at 104,149,755 bp (GRCm38)
  • A to G, chromosome 7 at 119,580,633 bp (GRCm38)
  • A to G, chromosome 7 at 120,189,782 bp (GRCm38)
  • T to C, chromosome 8 at 70,107,998 bp (GRCm38)
  • C to T, chromosome 8 at 70,709,145 bp (GRCm38)
  • T to C, chromosome 8 at 93,328,490 bp (GRCm38)
  • T to G, chromosome 8 at 94,356,400 bp (GRCm38)
  • T to C, chromosome 9 at 21,991,911 bp (GRCm38)
  • A to G, chromosome 9 at 38,253,666 bp (GRCm38)
  • T to C, chromosome 9 at 57,434,997 bp (GRCm38)
  • T to C, chromosome 9 at 109,484,372 bp (GRCm38)
  • T to A, chromosome 9 at 112,157,697 bp (GRCm38)
  • G to A, chromosome 10 at 94,944,823 bp (GRCm38)
  • A to T, chromosome 11 at 46,404,983 bp (GRCm38)
  • T to A, chromosome 11 at 59,543,315 bp (GRCm38)
  • G to A, chromosome 11 at 70,611,651 bp (GRCm38)
  • T to A, chromosome 11 at 77,113,720 bp (GRCm38)
  • A to G, chromosome 11 at 84,834,348 bp (GRCm38)
  • G to A, chromosome 11 at 100,447,655 bp (GRCm38)
  • A to G, chromosome 11 at 119,376,904 bp (GRCm38)
  • A to C, chromosome 12 at 72,449,397 bp (GRCm38)
  • T to A, chromosome 12 at 88,271,939 bp (GRCm38)
  • T to A, chromosome 12 at 115,508,025 bp (GRCm38)
  • G to A, chromosome 13 at 41,049,363 bp (GRCm38)
  • A to G, chromosome 13 at 64,058,645 bp (GRCm38)
  • G to A, chromosome 13 at 117,975,365 bp (GRCm38)
  • T to G, chromosome 14 at 7,791,765 bp (GRCm38)
  • A to T, chromosome 14 at 21,985,285 bp (GRCm38)
  • C to A, chromosome 14 at 47,710,896 bp (GRCm38)
  • A to G, chromosome 14 at 119,075,329 bp (GRCm38)
  • T to C, chromosome 15 at 36,087,398 bp (GRCm38)
  • T to A, chromosome 15 at 79,094,223 bp (GRCm38)
  • A to G, chromosome 15 at 101,463,454 bp (GRCm38)
  • T to C, chromosome 16 at 30,308,990 bp (GRCm38)
  • A to G, chromosome 16 at 45,775,074 bp (GRCm38)
  • T to A, chromosome 17 at 18,822,649 bp (GRCm38)
  • T to C, chromosome 17 at 35,853,366 bp (GRCm38)
  • C to A, chromosome 17 at 47,375,978 bp (GRCm38)
  • A to T, chromosome 18 at 35,657,011 bp (GRCm38)
  • A to G, chromosome 19 at 12,283,575 bp (GRCm38)
  • T to C, chromosome 19 at 47,015,377 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9324 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069136-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.