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Strain Name:
C57BL/6J-MtgxR9331Btlr/Mmmh
Stock Number:
069143-MU
Citation ID:
RRID:MMRRC_069143-MU
Other Names:
R9331 (G1)
Major Collection:

Strain Information

Nos1
Name: nitric oxide synthase 1, neuronal
Synonyms: nNOS, bNOS, Nos-1, NO, 2310005C01Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18125
HGNC: HGNC:7872
Homologene: 37327
Npy2r
Name: neuropeptide Y receptor Y2
Synonyms: NPY-Y2 receptor
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18167
HGNC: HGNC:7957
Homologene: 701
P2rx7
Name: purinergic receptor P2X, ligand-gated ion channel, 7
Synonyms: P2X7 receptor, P2X7R, P2X(7)
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18439
HGNC: HGNC:8537
Homologene: 1925
Trmo
Name: tRNA methyltransferase O
Synonyms: 5830415F09Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 74753
Homologene: 32316
Hcn4
Name: hyperpolarization-activated, cyclic nucleotide-gated K+ 4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 330953
VEGA: 9
Homologene: 3997
Kif20a
Name: kinesin family member 20A
Synonyms: Rabkinesin-6, Rab6kifl
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 19348
VEGA: 18
HGNC: HGNC:9787
Homologene: 38093
Diaph3
Name: diaphanous related formin 3
Synonyms: p134MDia2, mDia2, 4930417P13Rik, Drf3, Diap3
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 56419
VEGA: 14
Homologene: 84829
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • T to A, chromosome 1 at 17,762,230 bp (GRCm38)
  • T to C, chromosome 1 at 26,683,709 bp (GRCm38)
  • T to C, chromosome 1 at 55,696,871 bp (GRCm38)
  • A to G, chromosome 1 at 87,974,106 bp (GRCm38)
  • C to T, chromosome 1 at 167,308,563 bp (GRCm38)
  • T to C, chromosome 1 at 191,345,121 bp (GRCm38)
  • A to G, chromosome 2 at 26,597,306 bp (GRCm38)
  • CGCGGCGGCGGCGGCGGC to CGCGGCGGCGGCGGC, chromosome 2 at 74,705,598 bp (GRCm38)
  • A to T, chromosome 2 at 163,453,744 bp (GRCm38)
  • A to G, chromosome 2 at 174,455,679 bp (GRCm38)
  • T to C, chromosome 3 at 30,597,901 bp (GRCm38)
  • A to G, chromosome 3 at 35,960,089 bp (GRCm38)
  • A to G, chromosome 3 at 82,540,761 bp (GRCm38)
  • T to A, chromosome 4 at 43,454,320 bp (GRCm38)
  • A to G, chromosome 4 at 44,066,845 bp (GRCm38)
  • A to C, chromosome 4 at 46,387,642 bp (GRCm38)
  • T to C, chromosome 5 at 24,445,987 bp (GRCm38)
  • T to C, chromosome 5 at 117,900,524 bp (GRCm38)
  • T to C, chromosome 5 at 122,680,898 bp (GRCm38)
  • A to T, chromosome 6 at 24,765,867 bp (GRCm38)
  • C to T, chromosome 6 at 119,107,948 bp (GRCm38)
  • G to A, chromosome 7 at 7,464,403 bp (GRCm38)
  • C to T, chromosome 7 at 43,938,366 bp (GRCm38)
  • G to T, chromosome 7 at 45,614,190 bp (GRCm38)
  • T to C, chromosome 7 at 79,458,400 bp (GRCm38)
  • G to A, chromosome 7 at 139,255,441 bp (GRCm38)
  • C to A, chromosome 7 at 141,857,738 bp (GRCm38)
  • A to G, chromosome 8 at 24,687,935 bp (GRCm38)
  • C to T, chromosome 8 at 84,415,817 bp (GRCm38)
  • A to T, chromosome 9 at 30,951,474 bp (GRCm38)
  • A to G, chromosome 9 at 36,789,921 bp (GRCm38)
  • G to A, chromosome 9 at 37,855,414 bp (GRCm38)
  • A to G, chromosome 9 at 38,367,707 bp (GRCm38)
  • G to A, chromosome 9 at 50,770,211 bp (GRCm38)
  • T to C, chromosome 9 at 58,860,422 bp (GRCm38)
  • C to A, chromosome 9 at 65,556,584 bp (GRCm38)
  • G to A, chromosome 9 at 107,544,756 bp (GRCm38)
  • C to G, chromosome 10 at 5,123,666 bp (GRCm38)
  • A to T, chromosome 10 at 62,400,335 bp (GRCm38)
  • A to T, chromosome 10 at 128,487,441 bp (GRCm38)
  • T to C, chromosome 11 at 72,306,644 bp (GRCm38)
  • C to T, chromosome 11 at 103,117,148 bp (GRCm38)
  • A to G, chromosome 11 at 110,026,328 bp (GRCm38)
  • C to A, chromosome 12 at 57,673,723 bp (GRCm38)
  • C to T, chromosome 12 at 84,876,191 bp (GRCm38)
  • T to C, chromosome 12 at 113,736,258 bp (GRCm38)
  • C to T, chromosome 12 at 116,047,813 bp (GRCm38)
  • A to G, chromosome 13 at 27,665,079 bp (GRCm38)
  • T to A, chromosome 13 at 67,366,270 bp (GRCm38)
  • A to G, chromosome 14 at 21,045,598 bp (GRCm38)
  • T to C, chromosome 14 at 26,668,673 bp (GRCm38)
  • G to T, chromosome 14 at 87,141,025 bp (GRCm38)
  • T to A, chromosome 15 at 98,543,216 bp (GRCm38)
  • A to G, chromosome 17 at 24,397,350 bp (GRCm38)
  • G to A, chromosome 17 at 25,231,749 bp (GRCm38)
  • G to A, chromosome 17 at 40,578,729 bp (GRCm38)
  • T to A, chromosome 18 at 34,629,509 bp (GRCm38)
  • T to A, chromosome 18 at 44,837,432 bp (GRCm38)
  • T to C, chromosome 19 at 6,398,099 bp (GRCm38)
  • T to A, chromosome 19 at 23,694,343 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9331 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069143-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.