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Strain Name:
C57BL/6J-MtgxR9332Btlr/Mmmh
Stock Number:
069144-MU
Citation ID:
RRID:MMRRC_069144-MU
Other Names:
R9332 (G1)
Major Collection:

Strain Information

Tpst1
Name: protein-tyrosine sulfotransferase 1
Synonyms: Tango13a
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 22021
Homologene: 2667
Herc4
Name: hect domain and RLD 4
Synonyms: 4921531D01Rik, 1700056O17Rik, 9530080M15Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 67345
VEGA: 10
Homologene: 56715
Map4
Name: microtubule-associated protein 4
Synonyms: MAP 4, Mtap4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17758
HGNC: HGNC:6862
Homologene: 1780
Kat6b
Name: K(lysine) acetyltransferase 6B
Synonyms: qkf, querkopf, Morf, B130044K16Rik, monocytic leukemia, Myst4
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 54169
Homologene: 136480
Dennd2c
Name: DENN domain containing 2C
Synonyms: A930010I20Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 329727
Homologene: 19542
Mettl3
Name: methyltransferase 3, N6-adenosine-methyltransferase complex catalytic subunit
Synonyms: 2310024F18Rik, Spo8, M6A
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 56335
Homologene: 10501
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • C to T, chromosome 1 at 34,537,372 bp (GRCm38)
  • T to C, chromosome 1 at 54,277,751 bp (GRCm38)
  • T to A, chromosome 1 at 120,227,834 bp (GRCm38)
  • C to T, chromosome 1 at 136,092,714 bp (GRCm38)
  • T to A, chromosome 1 at 163,936,438 bp (GRCm38)
  • T to C, chromosome 1 at 175,772,305 bp (GRCm38)
  • A to T, chromosome 1 at 178,319,206 bp (GRCm38)
  • T to G, chromosome 1 at 182,157,437 bp (GRCm38)
  • T to C, chromosome 2 at 4,752,720 bp (GRCm38)
  • C to T, chromosome 2 at 65,103,172 bp (GRCm38)
  • A to T, chromosome 2 at 80,344,983 bp (GRCm38)
  • T to A, chromosome 2 at 85,607,987 bp (GRCm38)
  • A to G, chromosome 2 at 103,773,045 bp (GRCm38)
  • T to A, chromosome 2 at 125,678,698 bp (GRCm38)
  • G to A, chromosome 2 at 132,004,406 bp (GRCm38)
  • C to T, chromosome 2 at 155,628,802 bp (GRCm38)
  • T to G, chromosome 2 at 172,427,886 bp (GRCm38)
  • A to G, chromosome 2 at 174,423,185 bp (GRCm38)
  • A to G, chromosome 2 at 175,175,817 bp (GRCm38)
  • G to A, chromosome 3 at 37,050,840 bp (GRCm38)
  • G to A, chromosome 3 at 55,463,079 bp (GRCm38)
  • A to T, chromosome 3 at 64,547,250 bp (GRCm38)
  • T to C, chromosome 3 at 83,069,001 bp (GRCm38)
  • T to A, chromosome 3 at 103,131,561 bp (GRCm38)
  • A to G, chromosome 3 at 130,007,571 bp (GRCm38)
  • A to G, chromosome 4 at 11,304,298 bp (GRCm38)
  • T to A, chromosome 4 at 14,774,987 bp (GRCm38)
  • T to A, chromosome 4 at 99,008,043 bp (GRCm38)
  • C to T, chromosome 4 at 101,837,947 bp (GRCm38)
  • C to A, chromosome 4 at 130,771,898 bp (GRCm38)
  • T to C, chromosome 4 at 151,144,017 bp (GRCm38)
  • G to T, chromosome 5 at 30,956,329 bp (GRCm38)
  • T to A, chromosome 5 at 52,405,057 bp (GRCm38)
  • T to C, chromosome 5 at 114,611,337 bp (GRCm38)
  • C to T, chromosome 5 at 121,785,362 bp (GRCm38)
  • T to G, chromosome 5 at 128,601,252 bp (GRCm38)
  • C to A, chromosome 5 at 130,134,460 bp (GRCm38)
  • G to A, chromosome 6 at 40,004,247 bp (GRCm38)
  • A to G, chromosome 6 at 118,428,930 bp (GRCm38)
  • T to C, chromosome 7 at 26,459,652 bp (GRCm38)
  • A to T, chromosome 7 at 42,578,480 bp (GRCm38)
  • A to G, chromosome 7 at 43,299,423 bp (GRCm38)
  • A to G, chromosome 7 at 120,884,695 bp (GRCm38)
  • T to A, chromosome 7 at 127,559,640 bp (GRCm38)
  • A to T, chromosome 7 at 130,962,121 bp (GRCm38)
  • AGCAGCATCTGCTCG to AG, chromosome 8 at 26,160,854 bp (GRCm38)
  • CAGCATCTGCTCGGAGCA to CAGCA, chromosome 8 at 26,160,856 bp (GRCm38)
  • A to G, chromosome 8 at 71,359,602 bp (GRCm38)
  • C to A, chromosome 9 at 22,108,054 bp (GRCm38)
  • T to A, chromosome 9 at 42,001,518 bp (GRCm38)
  • C to A, chromosome 9 at 42,372,897 bp (GRCm38)
  • T to G, chromosome 9 at 67,831,497 bp (GRCm38)
  • G to T, chromosome 9 at 82,875,359 bp (GRCm38)
  • A to T, chromosome 9 at 108,067,505 bp (GRCm38)
  • A to T, chromosome 9 at 109,623,613 bp (GRCm38)
  • A to G, chromosome 9 at 110,035,155 bp (GRCm38)
  • G to T, chromosome 10 at 23,113,946 bp (GRCm38)
  • G to A, chromosome 10 at 63,308,346 bp (GRCm38)
  • A to G, chromosome 10 at 129,251,103 bp (GRCm38)
  • C to T, chromosome 11 at 21,711,522 bp (GRCm38)
  • T to A, chromosome 11 at 62,857,759 bp (GRCm38)
  • G to A, chromosome 11 at 67,179,383 bp (GRCm38)
  • A to G, chromosome 11 at 67,363,283 bp (GRCm38)
  • G to T, chromosome 11 at 87,167,502 bp (GRCm38)
  • G to T, chromosome 11 at 97,338,725 bp (GRCm38)
  • G to T, chromosome 11 at 114,789,207 bp (GRCm38)
  • T to G, chromosome 11 at 117,860,839 bp (GRCm38)
  • G to A, chromosome 12 at 51,899,285 bp (GRCm38)
  • T to A, chromosome 12 at 109,590,857 bp (GRCm38)
  • T to A, chromosome 13 at 21,981,682 bp (GRCm38)
  • T to A, chromosome 13 at 67,317,399 bp (GRCm38)
  • T to C, chromosome 13 at 73,511,343 bp (GRCm38)
  • T to G, chromosome 13 at 112,323,143 bp (GRCm38)
  • A to G, chromosome 14 at 21,670,025 bp (GRCm38)
  • A to T, chromosome 14 at 35,323,403 bp (GRCm38)
  • C to A, chromosome 14 at 52,296,668 bp (GRCm38)
  • C to A, chromosome 14 at 53,946,391 bp (GRCm38)
  • G to A, chromosome 15 at 89,355,374 bp (GRCm38)
  • G to T, chromosome 15 at 92,397,335 bp (GRCm38)
  • C to A, chromosome 17 at 4,995,309 bp (GRCm38)
  • T to A, chromosome 17 at 23,834,749 bp (GRCm38)
  • T to A, chromosome 18 at 10,532,796 bp (GRCm38)
  • A to G, chromosome 19 at 38,737,933 bp (GRCm38)
  • G to A, chromosome Y at 1,341,873 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9332 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069144-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.