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Strain Name:
C57BL/6J-MtgxR9457Btlr/Mmmh
Stock Number:
069260-MU
Citation ID:
RRID:MMRRC_069260-MU
Other Names:
R9457 (G1)
Major Collection:

Strain Information

Arhgap10
Name: Rho GTPase activating protein 10
Synonyms: PSGAP-s, PSGAP-m, A930033B01Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 78514
Homologene: 12695
Acadl
Name: acyl-Coenzyme A dehydrogenase, long-chain
Synonyms: LCAD, C79855
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 11363
HGNC: HGNC:88
Homologene: 37498
Lyst
Name: lysosomal trafficking regulator
Synonyms: D13Sfk13
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17101
VEGA: 13
HGNC: HGNC:1968
Homologene: 61
Scrib
Name: scribbled planar cell polarity
Synonyms: Scrb1, Crc
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 105782
Homologene: 44228
Snx4
Name: sorting nexin 4
Synonyms: 1810036H14Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 69150
VEGA: 16
Homologene: 36143
Tnrc6a
Name: trinucleotide repeat containing 6a
Synonyms: CAGH26, 3110054G10Rik, D130023A07Rik, 2010321I05Rik, Tnrc6
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233833
Homologene: 41399
Mllt6
Name: myeloid/lymphoid or mixed-lineage leukemia; translocated to, 6
Synonyms: Af17
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 246198
HGNC: HGNC:7138
Homologene: 31347
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • C to T, chromosome 1 at 45,386,844 bp (GRCm38)
  • A to G, chromosome 1 at 45,392,813 bp (GRCm38)
  • A to G, chromosome 1 at 66,853,241 bp (GRCm38)
  • G to A, chromosome 1 at 93,750,306 bp (GRCm38)
  • A to G, chromosome 1 at 151,198,475 bp (GRCm38)
  • T to C, chromosome 1 at 153,139,854 bp (GRCm38)
  • G to A, chromosome 2 at 25,457,718 bp (GRCm38)
  • A to G, chromosome 2 at 34,854,326 bp (GRCm38)
  • T to C, chromosome 2 at 67,515,632 bp (GRCm38)
  • T to A, chromosome 2 at 70,095,209 bp (GRCm38)
  • A to T, chromosome 2 at 86,236,472 bp (GRCm38)
  • A to T, chromosome 2 at 89,302,731 bp (GRCm38)
  • A to T, chromosome 2 at 111,170,286 bp (GRCm38)
  • T to A, chromosome 2 at 144,373,782 bp (GRCm38)
  • T to C, chromosome 2 at 146,334,554 bp (GRCm38)
  • T to A, chromosome 3 at 32,181,869 bp (GRCm38)
  • T to A, chromosome 3 at 54,789,335 bp (GRCm38)
  • T to A, chromosome 4 at 11,771,329 bp (GRCm38)
  • A to C, chromosome 4 at 94,586,883 bp (GRCm38)
  • C to T, chromosome 4 at 96,307,359 bp (GRCm38)
  • A to T, chromosome 4 at 108,217,905 bp (GRCm38)
  • G to A, chromosome 5 at 29,206,186 bp (GRCm38)
  • T to G, chromosome 5 at 34,301,397 bp (GRCm38)
  • G to T, chromosome 5 at 41,821,967 bp (GRCm38)
  • T to C, chromosome 5 at 89,214,573 bp (GRCm38)
  • A to T, chromosome 5 at 134,502,730 bp (GRCm38)
  • T to C, chromosome 5 at 135,349,632 bp (GRCm38)
  • T to A, chromosome 5 at 144,826,668 bp (GRCm38)
  • A to G, chromosome 6 at 57,024,523 bp (GRCm38)
  • T to C, chromosome 6 at 87,933,274 bp (GRCm38)
  • G to A, chromosome 6 at 122,954,086 bp (GRCm38)
  • T to G, chromosome 7 at 6,707,999 bp (GRCm38)
  • C to T, chromosome 7 at 16,434,750 bp (GRCm38)
  • C to A, chromosome 7 at 66,172,400 bp (GRCm38)
  • T to A, chromosome 7 at 103,451,411 bp (GRCm38)
  • A to G, chromosome 7 at 112,411,458 bp (GRCm38)
  • G to A, chromosome 7 at 123,179,735 bp (GRCm38)
  • T to C, chromosome 7 at 143,883,976 bp (GRCm38)
  • G to A, chromosome 8 at 77,384,786 bp (GRCm38)
  • T to A, chromosome 8 at 104,629,727 bp (GRCm38)
  • T to A, chromosome 9 at 61,944,225 bp (GRCm38)
  • T to A, chromosome 10 at 59,441,683 bp (GRCm38)
  • G to A, chromosome 10 at 62,950,793 bp (GRCm38)
  • C to T, chromosome 10 at 75,815,125 bp (GRCm38)
  • G to A, chromosome 10 at 77,049,771 bp (GRCm38)
  • A to T, chromosome 10 at 77,322,148 bp (GRCm38)
  • T to C, chromosome 10 at 77,911,392 bp (GRCm38)
  • C to A, chromosome 10 at 81,365,777 bp (GRCm38)
  • A to G, chromosome 10 at 82,286,739 bp (GRCm38)
  • T to C, chromosome 10 at 115,578,331 bp (GRCm38)
  • A to G, chromosome 11 at 3,676,184 bp (GRCm38)
  • G to T, chromosome 11 at 83,017,706 bp (GRCm38)
  • T to C, chromosome 11 at 97,665,760 bp (GRCm38)
  • T to G, chromosome 11 at 102,769,898 bp (GRCm38)
  • A to G, chromosome 11 at 105,994,861 bp (GRCm38)
  • T to C, chromosome 12 at 40,104,958 bp (GRCm38)
  • A to T, chromosome 12 at 75,415,749 bp (GRCm38)
  • T to A, chromosome 12 at 81,379,246 bp (GRCm38)
  • A to T, chromosome 12 at 84,789,153 bp (GRCm38)
  • A to G, chromosome 13 at 13,687,745 bp (GRCm38)
  • A to G, chromosome 13 at 55,161,127 bp (GRCm38)
  • G to A, chromosome 13 at 73,681,765 bp (GRCm38)
  • A to T, chromosome 13 at 76,384,674 bp (GRCm38)
  • A to T, chromosome 13 at 92,345,086 bp (GRCm38)
  • A to T, chromosome 14 at 51,456,012 bp (GRCm38)
  • T to A, chromosome 15 at 58,790,179 bp (GRCm38)
  • T to C, chromosome 15 at 76,067,299 bp (GRCm38)
  • A to G, chromosome 15 at 77,959,694 bp (GRCm38)
  • T to A, chromosome 15 at 84,207,841 bp (GRCm38)
  • A to G, chromosome 15 at 100,806,260 bp (GRCm38)
  • T to A, chromosome 16 at 18,540,995 bp (GRCm38)
  • A to G, chromosome 16 at 33,286,010 bp (GRCm38)
  • A to T, chromosome 16 at 55,813,984 bp (GRCm38)
  • CA to CAA, chromosome 17 at 24,527,763 bp (GRCm38)
  • A to G, chromosome 17 at 33,962,182 bp (GRCm38)
  • C to T, chromosome 17 at 35,682,758 bp (GRCm38)
  • A to G, chromosome 18 at 12,179,246 bp (GRCm38)
  • A to G, chromosome 18 at 61,845,849 bp (GRCm38)
  • A to G, chromosome 19 at 4,841,631 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9457 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069260-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.