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Strain Name:
C57BL/6J-MtgxR9469Btlr/Mmmh
Stock Number:
069272-MU
Citation ID:
RRID:MMRRC_069272-MU
Other Names:
R9469 (G1)
Major Collection:

Strain Information

Pou2f1
Name: POU domain, class 2, transcription factor 1
Synonyms: Oct-1C, Oct-1B, Oct-1A, oct-1, Otf-1, Otf1, 2810482H01Rik, Oct-1z, Oct1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18986
HGNC: HGNC:9212
Homologene: 37658
Nrp2
Name: neuropilin 2
Synonyms: NP2, Npn-2, Npn2, NP-2, 1110048P06Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18187
HGNC: HGNC:8005
Homologene: 2875
Zfp423
Name: zinc finger protein 423
Synonyms: Roaz, ataxia1, Zfp104, Ebfaz
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 94187
Homologene: 9010
Mrpl39
Name: mitochondrial ribosomal protein L39
Synonyms: MRP-L5, Rpml5
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 27393
Homologene: 9679
Adam23
Name: a disintegrin and metallopeptidase domain 23
Synonyms: MDC3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 23792
HGNC: HGNC:202
Homologene: 2826
Tnfaip1
Name: tumor necrosis factor, alpha-induced protein 1 (endothelial)
Synonyms: Edp-1, Edp1, Tnfip1, Bacurd2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 21927
Homologene: 22519
Ticrr
Name: TOPBP1-interacting checkpoint and replication regulator
Synonyms: 5730590G19Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 77011
Homologene: 67120
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • A to C, chromosome 1 at 59,842,769 bp (GRCm38)
  • T to C, chromosome 1 at 62,764,871 bp (GRCm38)
  • A to G, chromosome 1 at 63,545,512 bp (GRCm38)
  • A to T, chromosome 1 at 84,922,891 bp (GRCm38)
  • A to G, chromosome 1 at 90,928,346 bp (GRCm38)
  • T to A, chromosome 1 at 128,179,184 bp (GRCm38)
  • A to G, chromosome 1 at 151,193,312 bp (GRCm38)
  • A to G, chromosome 1 at 165,306,327 bp (GRCm38)
  • G to A, chromosome 1 at 165,913,261 bp (GRCm38)
  • G to A, chromosome 2 at 26,007,751 bp (GRCm38)
  • G to A, chromosome 2 at 65,136,430 bp (GRCm38)
  • C to A, chromosome 2 at 86,445,443 bp (GRCm38)
  • T to C, chromosome 2 at 102,400,306 bp (GRCm38)
  • A to G, chromosome 2 at 127,465,402 bp (GRCm38)
  • T to A, chromosome 2 at 163,732,272 bp (GRCm38)
  • T to C, chromosome 2 at 170,349,372 bp (GRCm38)
  • A to G, chromosome 3 at 131,583,198 bp (GRCm38)
  • T to C, chromosome 3 at 144,802,177 bp (GRCm38)
  • T to C, chromosome 4 at 16,138,181 bp (GRCm38)
  • G to A, chromosome 4 at 96,629,494 bp (GRCm38)
  • A to G, chromosome 4 at 100,549,761 bp (GRCm38)
  • G to A, chromosome 4 at 119,658,620 bp (GRCm38)
  • A to G, chromosome 4 at 145,054,121 bp (GRCm38)
  • G to T, chromosome 4 at 145,701,117 bp (GRCm38)
  • A to G, chromosome 4 at 154,250,912 bp (GRCm38)
  • G to A, chromosome 5 at 90,462,800 bp (GRCm38)
  • T to C, chromosome 5 at 112,846,381 bp (GRCm38)
  • G to A, chromosome 6 at 32,517,591 bp (GRCm38)
  • A to G, chromosome 6 at 42,596,894 bp (GRCm38)
  • A to G, chromosome 6 at 88,205,319 bp (GRCm38)
  • G to A, chromosome 6 at 115,742,360 bp (GRCm38)
  • C to A, chromosome 6 at 129,906,723 bp (GRCm38)
  • A to T, chromosome 7 at 5,484,119 bp (GRCm38)
  • A to T, chromosome 7 at 15,950,407 bp (GRCm38)
  • A to T, chromosome 7 at 29,329,056 bp (GRCm38)
  • T to C, chromosome 7 at 35,644,402 bp (GRCm38)
  • C to T, chromosome 7 at 79,694,763 bp (GRCm38)
  • A to T, chromosome 7 at 99,926,360 bp (GRCm38)
  • A to T, chromosome 7 at 105,180,760 bp (GRCm38)
  • T to C, chromosome 7 at 118,140,551 bp (GRCm38)
  • T to A, chromosome 7 at 127,596,597 bp (GRCm38)
  • C to A, chromosome 7 at 131,336,663 bp (GRCm38)
  • C to T, chromosome 8 at 13,372,965 bp (GRCm38)
  • G to A, chromosome 8 at 64,743,035 bp (GRCm38)
  • T to A, chromosome 8 at 87,782,891 bp (GRCm38)
  • T to C, chromosome 8 at 110,882,172 bp (GRCm38)
  • T to C, chromosome 9 at 52,065,769 bp (GRCm38)
  • A to T, chromosome 9 at 122,748,520 bp (GRCm38)
  • A to G, chromosome 10 at 71,366,013 bp (GRCm38)
  • A to T, chromosome 10 at 90,897,343 bp (GRCm38)
  • A to G, chromosome 10 at 103,214,900 bp (GRCm38)
  • T to A, chromosome 11 at 6,273,763 bp (GRCm38)
  • A to G, chromosome 11 at 8,998,744 bp (GRCm38)
  • T to C, chromosome 11 at 53,723,997 bp (GRCm38)
  • A to G, chromosome 11 at 65,196,394 bp (GRCm38)
  • G to A, chromosome 11 at 69,431,070 bp (GRCm38)
  • A to G, chromosome 11 at 78,528,249 bp (GRCm38)
  • G to A, chromosome 11 at 82,795,373 bp (GRCm38)
  • T to C, chromosome 11 at 103,208,915 bp (GRCm38)
  • G to A, chromosome 11 at 106,272,506 bp (GRCm38)
  • G to T, chromosome 13 at 19,086,599 bp (GRCm38)
  • G to A, chromosome 13 at 23,011,931 bp (GRCm38)
  • A to G, chromosome 13 at 67,786,206 bp (GRCm38)
  • A to G, chromosome 14 at 59,791,219 bp (GRCm38)
  • A to T, chromosome 15 at 28,421,000 bp (GRCm38)
  • A to G, chromosome 15 at 75,667,893 bp (GRCm38)
  • T to G, chromosome 16 at 84,720,282 bp (GRCm38)
  • T to A, chromosome 17 at 25,491,967 bp (GRCm38)
  • C to A, chromosome 17 at 28,167,164 bp (GRCm38)
  • T to C, chromosome 17 at 37,300,065 bp (GRCm38)
  • G to A, chromosome 17 at 50,606,925 bp (GRCm38)
  • A to T, chromosome 17 at 56,876,283 bp (GRCm38)
  • G to T, chromosome 17 at 71,061,127 bp (GRCm38)
  • T to C, chromosome 18 at 20,267,890 bp (GRCm38)
  • A to G, chromosome 18 at 22,952,261 bp (GRCm38)
  • T to C, chromosome 18 at 44,886,316 bp (GRCm38)
  • T to A, chromosome 18 at 67,675,837 bp (GRCm38)
  • G to A, chromosome 19 at 9,010,861 bp (GRCm38)
  • G to A, chromosome 19 at 12,088,070 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9469 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069272-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.