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Strain Name:
C57BL/6J-MtgxR9473Btlr/Mmmh
Stock Number:
069276-MU
Citation ID:
RRID:MMRRC_069276-MU
Other Names:
R9473 (G1)
Major Collection:

Strain Information

Ubr5
Name: ubiquitin protein ligase E3 component n-recognin 5
Synonyms: 4432411E13Rik, Edd1, Edd
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 70790
VEGA: 15
Homologene: 9295
Atm
Name: ataxia telangiectasia mutated
Synonyms: C030026E19Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 11920
HGNC: HGNC:795
Homologene: 30952
Pip5k1b
Name: phosphatidylinositol-4-phosphate 5-kinase, type 1 beta
Synonyms: Pipk5b
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 18719
HGNC: HGNC:8995
Homologene: 100644
Ttc39b
Name: tetratricopeptide repeat domain 39B
Synonyms: 9130422G05Rik, 1810054D07Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 69863
Homologene: 25228
Rfx1
Name: regulatory factor X, 1 (influences HLA class II expression)
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 19724
HGNC: HGNC:9982
Homologene: 2189
Eif3l
Name: eukaryotic translation initiation factor 3, subunit L
Synonyms: PAF67, 0610011H21Rik, HSP-66Y, Eif3s6ip, Eif3eip, D15N1e
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223691
VEGA: 15
Homologene: 9380
Gtf2ird1
Name: general transcription factor II I repeat domain-containing 1
Synonyms: WBSCR11, Cream1, GTF3, MusTRD1, binding factor for early enhancer, BEN, ESTM9
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 57080
HGNC: HGNC:4661
Homologene: 4158
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • A to G, chromosome 2 at 76,716,994 bp (GRCm38)
  • T to C, chromosome 2 at 93,855,747 bp (GRCm38)
  • A to C, chromosome 2 at 118,532,623 bp (GRCm38)
  • G to T, chromosome 2 at 120,496,054 bp (GRCm38)
  • A to G, chromosome 2 at 122,303,845 bp (GRCm38)
  • A to G, chromosome 4 at 41,757,575 bp (GRCm38)
  • A to T, chromosome 4 at 43,514,813 bp (GRCm38)
  • A to T, chromosome 4 at 48,052,143 bp (GRCm38)
  • A to G, chromosome 4 at 58,064,243 bp (GRCm38)
  • A to G, chromosome 4 at 83,263,740 bp (GRCm38)
  • T to G, chromosome 4 at 143,894,245 bp (GRCm38)
  • T to C, chromosome 5 at 5,740,378 bp (GRCm38)
  • T to C, chromosome 5 at 21,585,245 bp (GRCm38)
  • C to T, chromosome 5 at 74,585,058 bp (GRCm38)
  • A to T, chromosome 5 at 86,917,680 bp (GRCm38)
  • G to A, chromosome 5 at 124,574,614 bp (GRCm38)
  • T to A, chromosome 5 at 134,404,680 bp (GRCm38)
  • C to T, chromosome 5 at 145,044,102 bp (GRCm38)
  • G to A, chromosome 6 at 54,689,024 bp (GRCm38)
  • T to C, chromosome 6 at 131,689,141 bp (GRCm38)
  • G to T, chromosome 7 at 18,013,356 bp (GRCm38)
  • G to A, chromosome 8 at 3,479,232 bp (GRCm38)
  • T to A, chromosome 8 at 27,120,915 bp (GRCm38)
  • T to C, chromosome 8 at 60,954,617 bp (GRCm38)
  • A to G, chromosome 8 at 79,775,044 bp (GRCm38)
  • A to T, chromosome 8 at 84,093,274 bp (GRCm38)
  • T to C, chromosome 8 at 92,902,226 bp (GRCm38)
  • C to T, chromosome 9 at 53,498,972 bp (GRCm38)
  • G to T, chromosome 9 at 78,448,557 bp (GRCm38)
  • T to C, chromosome 10 at 5,248,258 bp (GRCm38)
  • C to T, chromosome 10 at 78,050,188 bp (GRCm38)
  • A to G, chromosome 10 at 127,860,308 bp (GRCm38)
  • A to G, chromosome 11 at 71,788,818 bp (GRCm38)
  • T to A, chromosome 11 at 76,022,238 bp (GRCm38)
  • G to A, chromosome 11 at 78,284,157 bp (GRCm38)
  • T to C, chromosome 11 at 107,184,755 bp (GRCm38)
  • T to C, chromosome 12 at 76,329,858 bp (GRCm38)
  • T to C, chromosome 12 at 103,763,680 bp (GRCm38)
  • T to C, chromosome 12 at 114,223,102 bp (GRCm38)
  • A to G, chromosome 12 at 119,334,255 bp (GRCm38)
  • T to C, chromosome 13 at 23,959,748 bp (GRCm38)
  • T to C, chromosome 14 at 32,463,956 bp (GRCm38)
  • A to G, chromosome 15 at 38,002,373 bp (GRCm38)
  • G to A, chromosome 15 at 39,754,576 bp (GRCm38)
  • A to G, chromosome 15 at 39,824,187 bp (GRCm38)
  • T to A, chromosome 15 at 79,086,504 bp (GRCm38)
  • C to T, chromosome 15 at 101,420,528 bp (GRCm38)
  • G to A, chromosome 16 at 17,980,701 bp (GRCm38)
  • T to C, chromosome 17 at 46,306,683 bp (GRCm38)
  • T to G, chromosome 18 at 90,590,969 bp (GRCm38)
  • A to T, chromosome 19 at 24,358,226 bp (GRCm38)
  • G to A, chromosome 19 at 38,777,893 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9473 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069276-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.