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Strain Name:
C57BL/6J-MtgxR9477Btlr/Mmmh
Stock Number:
069280-MU
Citation ID:
RRID:MMRRC_069280-MU
Other Names:
R9477 (G1)
Major Collection:

Strain Information

Sptbn4
Name: spectrin beta, non-erythrocytic 4
Synonyms: dyn, SpbIV, neuroaxonal dystrophy, 5830426A08Rik, ROSA62, nmf261, 1700022P15Rik, Spnb4
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 80297
Homologene: 11879
Grm1
Name: glutamate receptor, metabotropic 1
Synonyms: mGluR1, Grm1, Gprc1a, nmf373, rcw, 4930455H15Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 14816
HGNC: HGNC:4593
Homologene: 649
Cdon
Name: cell adhesion molecule-related/down-regulated by oncogenes
Synonyms: CAM-related/down-regulated by oncogenes, CDO
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 57810
Homologene: 22996
Sema3c
Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C
Synonyms: Semae, 1110036B02Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 20348
Homologene: 36201
Vcan
Name: versican
Synonyms: PG-M, hdf, heart defect, 5430420N07Rik, Cspg2, DPEAAE
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 13003
HGNC: HGNC:2464
Homologene: 3228
App
Name: amyloid beta precursor protein
Synonyms: betaAPP, Abeta, protease nexin II, Adap, Cvap, appican, E030013M08Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 11820
VEGA: 16
HGNC: HGNC:620
Homologene: 56379
Pik3cb
Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta
Synonyms: 1110001J02Rik, p110beta
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 74769
HGNC: HGNC:8976
Homologene: 21250
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • T to G, chromosome 1 at 9,985,629 bp (GRCm38)
  • T to C, chromosome 1 at 17,746,732 bp (GRCm38)
  • A to G, chromosome 1 at 34,166,211 bp (GRCm38)
  • A to T, chromosome 1 at 89,837,763 bp (GRCm38)
  • A to G, chromosome 1 at 90,778,899 bp (GRCm38)
  • G to T, chromosome 1 at 117,801,173 bp (GRCm38)
  • G to T, chromosome 1 at 123,376,641 bp (GRCm38)
  • C to T, chromosome 1 at 155,226,341 bp (GRCm38)
  • T to C, chromosome 1 at 166,399,835 bp (GRCm38)
  • G to A, chromosome 1 at 174,026,589 bp (GRCm38)
  • A to T, chromosome 2 at 31,396,019 bp (GRCm38)
  • T to A, chromosome 2 at 37,518,374 bp (GRCm38)
  • G to A, chromosome 2 at 52,111,330 bp (GRCm38)
  • G to A, chromosome 2 at 66,022,475 bp (GRCm38)
  • G to A, chromosome 2 at 89,023,271 bp (GRCm38)
  • A to T, chromosome 2 at 137,094,489 bp (GRCm38)
  • A to G, chromosome 2 at 146,143,112 bp (GRCm38)
  • A to G, chromosome 3 at 40,830,622 bp (GRCm38)
  • A to G, chromosome 3 at 60,613,348 bp (GRCm38)
  • A to T, chromosome 3 at 87,252,496 bp (GRCm38)
  • A to G, chromosome 4 at 11,528,753 bp (GRCm38)
  • T to C, chromosome 4 at 43,566,298 bp (GRCm38)
  • G to A, chromosome 5 at 17,716,983 bp (GRCm38)
  • T to C, chromosome 5 at 37,288,838 bp (GRCm38)
  • T to G, chromosome 5 at 148,077,930 bp (GRCm38)
  • A to T, chromosome 6 at 22,308,480 bp (GRCm38)
  • A to T, chromosome 6 at 48,690,380 bp (GRCm38)
  • A to T, chromosome 6 at 65,794,358 bp (GRCm38)
  • G to A, chromosome 6 at 120,743,782 bp (GRCm38)
  • A to T, chromosome 6 at 124,191,951 bp (GRCm38)
  • A to G, chromosome 6 at 124,522,496 bp (GRCm38)
  • A to G, chromosome 6 at 126,739,398 bp (GRCm38)
  • G to A, chromosome 6 at 128,400,182 bp (GRCm38)
  • T to G, chromosome 7 at 21,272,336 bp (GRCm38)
  • A to G, chromosome 7 at 27,433,199 bp (GRCm38)
  • T to C, chromosome 7 at 28,152,840 bp (GRCm38)
  • T to A, chromosome 7 at 28,326,075 bp (GRCm38)
  • T to C, chromosome 7 at 30,732,257 bp (GRCm38)
  • G to A, chromosome 7 at 44,312,918 bp (GRCm38)
  • C to T, chromosome 7 at 85,569,036 bp (GRCm38)
  • T to C, chromosome 8 at 66,418,890 bp (GRCm38)
  • T to C, chromosome 8 at 84,912,150 bp (GRCm38)
  • A to T, chromosome 8 at 104,833,924 bp (GRCm38)
  • T to A, chromosome 9 at 29,411,626 bp (GRCm38)
  • A to G, chromosome 9 at 35,491,905 bp (GRCm38)
  • T to C, chromosome 9 at 60,870,826 bp (GRCm38)
  • C to T, chromosome 9 at 99,040,920 bp (GRCm38)
  • C to A, chromosome 9 at 124,476,857 bp (GRCm38)
  • T to C, chromosome 10 at 10,719,661 bp (GRCm38)
  • C to T, chromosome 10 at 52,417,905 bp (GRCm38)
  • A to T, chromosome 10 at 67,157,955 bp (GRCm38)
  • C to T, chromosome 10 at 77,911,390 bp (GRCm38)
  • T to A, chromosome 10 at 114,503,075 bp (GRCm38)
  • T to A, chromosome 10 at 128,298,053 bp (GRCm38)
  • A to T, chromosome 11 at 104,945,872 bp (GRCm38)
  • A to G, chromosome 11 at 106,519,880 bp (GRCm38)
  • A to G, chromosome 12 at 31,930,767 bp (GRCm38)
  • A to G, chromosome 12 at 58,267,834 bp (GRCm38)
  • C to A, chromosome 13 at 67,492,039 bp (GRCm38)
  • T to A, chromosome 13 at 89,693,009 bp (GRCm38)
  • A to T, chromosome 13 at 120,026,746 bp (GRCm38)
  • A to G, chromosome 14 at 55,739,411 bp (GRCm38)
  • A to G, chromosome 14 at 56,131,356 bp (GRCm38)
  • T to C, chromosome 15 at 58,873,622 bp (GRCm38)
  • T to C, chromosome 15 at 100,038,903 bp (GRCm38)
  • T to A, chromosome 16 at 44,376,820 bp (GRCm38)
  • C to T, chromosome 16 at 65,523,617 bp (GRCm38)
  • C to A, chromosome 16 at 85,056,504 bp (GRCm38)
  • G to A, chromosome 17 at 27,118,677 bp (GRCm38)
  • T to C, chromosome 17 at 55,988,720 bp (GRCm38)
  • A to T, chromosome 17 at 56,062,244 bp (GRCm38)
  • A to T, chromosome 18 at 16,622,155 bp (GRCm38)
  • A to G, chromosome 18 at 24,003,131 bp (GRCm38)
  • A to C, chromosome 18 at 44,337,266 bp (GRCm38)
  • T to C, chromosome 18 at 65,644,357 bp (GRCm38)
  • G to A, chromosome 19 at 47,876,472 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9477 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069280-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.