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Strain Name:
C57BL/6J-MtgxR9497Btlr/Mmmh
Stock Number:
069300-MU
Citation ID:
RRID:MMRRC_069300-MU
Other Names:
R9497 (G1)
Major Collection:

Strain Information

Kif5a
Name: kinesin family member 5A
Synonyms: Kns, Kif5, D10Bwg0738e, Khc
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 16572
VEGA: 10
HGNC: HGNC:6323
Homologene: 55861
Myh7
Name: myosin, heavy polypeptide 7, cardiac muscle, beta
Synonyms: Myhc-b, Myhcb, beta-MHC, B-MHC, MYH-beta/slow, MyHC-I, betaMHC
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 140781
HGNC: HGNC:7577
Homologene: 68044
Htr1d
Name: 5-hydroxytryptamine (serotonin) receptor 1D
Synonyms: Gpcr14, Htr1db
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 15552
HGNC: HGNC:5289
Homologene: 20240
Ppp1r13b
Name: protein phosphatase 1, regulatory subunit 13B
Synonyms: ASPP1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 21981
VEGA: 12
Homologene: 9090
Fryl
Name: FRY like transcription coactivator
Synonyms: 2310004H21Rik, 2510002A14Rik, 9030227G01Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 72313
Homologene: 103956
Nfkbil1
Name: nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor like 1
Synonyms: Def-7
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18038
HGNC: HGNC:7800
Homologene: 3671
Mysm1
Name: myb-like, SWIRM and MPN domains 1
Synonyms: C530050H10Rik, C130067A03Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 320713
Homologene: 66204
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • C to T, chromosome 1 at 14,918,802 bp (GRCm38)
  • C to T, chromosome 1 at 75,490,840 bp (GRCm38)
  • A to C, chromosome 1 at 86,046,171 bp (GRCm38)
  • T to C, chromosome 1 at 152,757,196 bp (GRCm38)
  • A to G, chromosome 1 at 195,168,435 bp (GRCm38)
  • T to C, chromosome 2 at 21,827,014 bp (GRCm38)
  • A to G, chromosome 2 at 84,981,341 bp (GRCm38)
  • T to C, chromosome 2 at 115,864,243 bp (GRCm38)
  • T to C, chromosome 2 at 127,877,786 bp (GRCm38)
  • T to C, chromosome 2 at 155,406,318 bp (GRCm38)
  • T to C, chromosome 3 at 64,707,053 bp (GRCm38)
  • A to G, chromosome 3 at 97,051,485 bp (GRCm38)
  • A to T, chromosome 3 at 98,877,146 bp (GRCm38)
  • G to A, chromosome 3 at 129,905,910 bp (GRCm38)
  • T to G, chromosome 4 at 40,994,104 bp (GRCm38)
  • A to C, chromosome 4 at 72,152,898 bp (GRCm38)
  • T to C, chromosome 4 at 94,960,398 bp (GRCm38)
  • G to A, chromosome 4 at 96,571,974 bp (GRCm38)
  • A to T, chromosome 4 at 115,414,571 bp (GRCm38)
  • A to G, chromosome 4 at 136,442,352 bp (GRCm38)
  • T to C, chromosome 5 at 24,715,540 bp (GRCm38)
  • A to G, chromosome 5 at 45,733,997 bp (GRCm38)
  • T to C, chromosome 5 at 73,057,791 bp (GRCm38)
  • G to T, chromosome 5 at 96,737,036 bp (GRCm38)
  • C to T, chromosome 6 at 15,720,853 bp (GRCm38)
  • T to G, chromosome 6 at 15,770,509 bp (GRCm38)
  • T to C, chromosome 6 at 39,151,319 bp (GRCm38)
  • C to A, chromosome 6 at 72,429,207 bp (GRCm38)
  • C to T, chromosome 6 at 92,156,692 bp (GRCm38)
  • T to A, chromosome 6 at 96,843,870 bp (GRCm38)
  • G to T, chromosome 6 at 131,659,586 bp (GRCm38)
  • C to T, chromosome 6 at 146,623,681 bp (GRCm38)
  • A to T, chromosome 7 at 12,810,288 bp (GRCm38)
  • G to A, chromosome 7 at 27,155,292 bp (GRCm38)
  • A to T, chromosome 7 at 45,154,365 bp (GRCm38)
  • G to A, chromosome 7 at 86,321,781 bp (GRCm38)
  • A to G, chromosome 7 at 109,716,259 bp (GRCm38)
  • A to T, chromosome 7 at 130,164,033 bp (GRCm38)
  • A to T, chromosome 7 at 142,165,561 bp (GRCm38)
  • T to A, chromosome 8 at 25,727,704 bp (GRCm38)
  • A to T, chromosome 8 at 57,837,376 bp (GRCm38)
  • A to T, chromosome 8 at 77,720,305 bp (GRCm38)
  • G to A, chromosome 9 at 7,861,027 bp (GRCm38)
  • A to G, chromosome 9 at 15,992,208 bp (GRCm38)
  • A to T, chromosome 9 at 40,023,320 bp (GRCm38)
  • G to A, chromosome 9 at 40,100,639 bp (GRCm38)
  • T to C, chromosome 9 at 50,800,136 bp (GRCm38)
  • T to G, chromosome 9 at 71,162,369 bp (GRCm38)
  • T to A, chromosome 9 at 72,619,141 bp (GRCm38)
  • A to G, chromosome 9 at 107,984,739 bp (GRCm38)
  • C to T, chromosome 10 at 23,111,559 bp (GRCm38)
  • A to C, chromosome 10 at 36,833,374 bp (GRCm38)
  • A to T, chromosome 10 at 70,940,998 bp (GRCm38)
  • A to G, chromosome 10 at 78,519,904 bp (GRCm38)
  • T to C, chromosome 10 at 105,841,229 bp (GRCm38)
  • T to C, chromosome 10 at 127,243,484 bp (GRCm38)
  • C to A, chromosome 10 at 129,160,595 bp (GRCm38)
  • T to A, chromosome 11 at 12,253,501 bp (GRCm38)
  • A to G, chromosome 11 at 50,236,559 bp (GRCm38)
  • A to G, chromosome 11 at 75,169,305 bp (GRCm38)
  • T to C, chromosome 11 at 120,608,647 bp (GRCm38)
  • A to T, chromosome 12 at 50,391,324 bp (GRCm38)
  • A to C, chromosome 12 at 104,343,171 bp (GRCm38)
  • A to T, chromosome 12 at 111,841,012 bp (GRCm38)
  • C to A, chromosome 12 at 118,936,115 bp (GRCm38)
  • C to A, chromosome 13 at 36,898,144 bp (GRCm38)
  • A to T, chromosome 13 at 74,305,576 bp (GRCm38)
  • A to T, chromosome 13 at 92,474,113 bp (GRCm38)
  • G to T, chromosome 13 at 105,100,479 bp (GRCm38)
  • C to T, chromosome 14 at 50,298,905 bp (GRCm38)
  • A to T, chromosome 14 at 51,891,572 bp (GRCm38)
  • A to T, chromosome 14 at 54,980,384 bp (GRCm38)
  • A to G, chromosome 14 at 105,304,811 bp (GRCm38)
  • T to A, chromosome 15 at 4,921,363 bp (GRCm38)
  • A to T, chromosome 15 at 18,964,181 bp (GRCm38)
  • T to C, chromosome 15 at 89,388,260 bp (GRCm38)
  • T to A, chromosome 16 at 29,469,312 bp (GRCm38)
  • G to A, chromosome 17 at 35,220,629 bp (GRCm38)
  • T to C, chromosome 17 at 35,924,894 bp (GRCm38)
  • T to A, chromosome 17 at 37,752,051 bp (GRCm38)
  • G to T, chromosome 17 at 73,326,367 bp (GRCm38)
  • T to A, chromosome 18 at 10,458,600 bp (GRCm38)
  • T to C, chromosome 18 at 35,718,383 bp (GRCm38)
  • C to T, chromosome 19 at 44,021,646 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9497 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069300-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.