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Strain Name:
C57BL/6J-MtgxR9522Btlr/Mmmh
Stock Number:
069324-MU
Citation ID:
RRID:MMRRC_069324-MU
Other Names:
R9522 (G1)
Major Collection:

Strain Information

Setd5
Name: SET domain containing 5
Synonyms: 2900045N06Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 72895
Homologene: 12485
Map7
Name: microtubule-associated protein 7
Synonyms: E-MAP-115, mste, ste, mshi, Mtap7
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 17761
VEGA: 10
HGNC: HGNC:6869
Homologene: 20851
Eif3g
Name: eukaryotic translation initiation factor 3, subunit G
Synonyms: TU-189B2, p44, 44kDa, D0Jmb4, Eif3s4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 53356
VEGA: 9
HGNC: HGNC:3274
Homologene: 2784
Cep83
Name: centrosomal protein 83
Synonyms: 2600001G24Rik, 5730513H21Rik, 4921537D05Rik, Ccdc41
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 77048
VEGA: 10
Homologene: 9396
Rgs3
Name: regulator of G-protein signaling 3
Synonyms: 4930506N09Rik, C2pa, PDZ-RGS3, RGS3S, C2PA-RGS3
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 50780
HGNC: HGNC:9999
Homologene: 32440
Kif16b
Name: kinesin family member 16B
Synonyms: N-3 kinesin, 8430434E15Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16558
Homologene: 135708
Dlgap4
Name: DLG associated protein 4
Synonyms: WBP16, SAP90/PSD-95-associated protein 4, PSD-95/SAP90 binding protein 4, DAP4, Sapap4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228836
Homologene: 8935
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • A to T, chromosome 1 at 45,909,512 bp (GRCm38)
  • G to A, chromosome 1 at 66,638,062 bp (GRCm38)
  • T to C, chromosome 1 at 93,189,707 bp (GRCm38)
  • A to G, chromosome 1 at 100,484,622 bp (GRCm38)
  • G to A, chromosome 1 at 134,313,148 bp (GRCm38)
  • C to T, chromosome 1 at 157,564,765 bp (GRCm38)
  • T to C, chromosome 2 at 52,081,299 bp (GRCm38)
  • G to A, chromosome 2 at 87,990,831 bp (GRCm38)
  • A to G, chromosome 2 at 92,369,921 bp (GRCm38)
  • T to A, chromosome 2 at 112,730,414 bp (GRCm38)
  • A to T, chromosome 2 at 142,849,907 bp (GRCm38)
  • G to T, chromosome 2 at 156,704,594 bp (GRCm38)
  • G to A, chromosome 2 at 166,934,753 bp (GRCm38)
  • A to G, chromosome 2 at 172,427,428 bp (GRCm38)
  • T to C, chromosome 3 at 28,806,594 bp (GRCm38)
  • A to G, chromosome 3 at 36,182,527 bp (GRCm38)
  • A to G, chromosome 3 at 87,256,794 bp (GRCm38)
  • T to G, chromosome 3 at 95,546,798 bp (GRCm38)
  • T to C, chromosome 3 at 98,446,467 bp (GRCm38)
  • A to T, chromosome 3 at 107,672,082 bp (GRCm38)
  • A to G, chromosome 4 at 62,605,492 bp (GRCm38)
  • G to A, chromosome 4 at 137,613,685 bp (GRCm38)
  • G to A, chromosome 5 at 30,199,484 bp (GRCm38)
  • A to T, chromosome 5 at 30,466,123 bp (GRCm38)
  • C to A, chromosome 5 at 30,778,055 bp (GRCm38)
  • G to T, chromosome 5 at 139,805,730 bp (GRCm38)
  • T to A, chromosome 6 at 40,880,441 bp (GRCm38)
  • G to A, chromosome 6 at 90,309,524 bp (GRCm38)
  • T to G, chromosome 6 at 113,115,034 bp (GRCm38)
  • T to C, chromosome 7 at 12,769,370 bp (GRCm38)
  • T to C, chromosome 7 at 23,928,777 bp (GRCm38)
  • T to C, chromosome 7 at 43,675,476 bp (GRCm38)
  • G to T, chromosome 7 at 81,829,333 bp (GRCm38)
  • A to T, chromosome 7 at 120,248,145 bp (GRCm38)
  • T to A, chromosome 7 at 140,274,074 bp (GRCm38)
  • T to C, chromosome 8 at 45,277,015 bp (GRCm38)
  • C to T, chromosome 8 at 92,360,631 bp (GRCm38)
  • G to T, chromosome 9 at 20,898,155 bp (GRCm38)
  • T to A, chromosome 9 at 20,898,156 bp (GRCm38)
  • G to A, chromosome 9 at 21,394,237 bp (GRCm38)
  • A to G, chromosome 9 at 32,116,154 bp (GRCm38)
  • A to G, chromosome 9 at 59,353,408 bp (GRCm38)
  • A to G, chromosome 10 at 20,229,896 bp (GRCm38)
  • A to G, chromosome 10 at 94,750,322 bp (GRCm38)
  • T to C, chromosome 11 at 5,282,379 bp (GRCm38)
  • T to C, chromosome 11 at 83,212,999 bp (GRCm38)
  • C to A, chromosome 12 at 21,345,692 bp (GRCm38)
  • A to T, chromosome 12 at 28,570,654 bp (GRCm38)
  • A to G, chromosome 12 at 75,633,150 bp (GRCm38)
  • T to C, chromosome 13 at 59,716,966 bp (GRCm38)
  • A to T, chromosome 13 at 61,004,443 bp (GRCm38)
  • T to C, chromosome 14 at 15,358,423 bp (GRCm38)
  • A to T, chromosome 14 at 55,478,762 bp (GRCm38)
  • T to C, chromosome 14 at 63,159,148 bp (GRCm38)
  • C to A, chromosome 15 at 9,370,123 bp (GRCm38)
  • C to T, chromosome 15 at 64,920,711 bp (GRCm38)
  • T to C, chromosome 15 at 66,632,820 bp (GRCm38)
  • T to C, chromosome 15 at 75,667,460 bp (GRCm38)
  • G to A, chromosome 15 at 82,764,073 bp (GRCm38)
  • T to C, chromosome 16 at 22,822,499 bp (GRCm38)
  • T to C, chromosome 16 at 57,198,744 bp (GRCm38)
  • G to T, chromosome 17 at 12,698,328 bp (GRCm38)
  • C to T, chromosome 17 at 24,447,122 bp (GRCm38)
  • A to T, chromosome 17 at 38,335,429 bp (GRCm38)
  • A to G, chromosome 17 at 56,566,900 bp (GRCm38)
  • T to C, chromosome 19 at 4,627,246 bp (GRCm38)
  • T to A, chromosome 19 at 12,954,013 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9522 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069324-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.