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Strain Name:
C57BL/6J-MtgxR9547Btlr/Mmmh
Stock Number:
069343-MU
Citation ID:
RRID:MMRRC_069343-MU
Other Names:
R9547 (G1)
Major Collection:

Strain Information

Ednrb
Name: endothelin receptor type B
Synonyms: ETb, Sox10m1, ETR-b
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 13618
VEGA: 14
HGNC: HGNC:3180
Homologene: 89
Kdm4c
Name: lysine (K)-specific demethylase 4C
Synonyms: 2410141F18Rik, Jmjd2c
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 76804
Homologene: 41004
Nphs1
Name: nephrosis 1, nephrin
Synonyms: nephrin
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 54631
HGNC: HGNC:7908
Homologene: 20974
Srsf11
Name: serine and arginine-rich splicing factor 11
Synonyms: 0610009J05Rik, 2610019N13Rik, Sfrs11
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 69207
Homologene: 36164
Tex21
Name: testis expressed gene 21
Synonyms: tsec-2, 4931412D23Rik, 4931406F04Rik, 4931421K24Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 80384
VEGA: 12
Homologene: 10510
Anks1
Name: ankyrin repeat and SAM domain containing 1
Synonyms: Odin
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224650
Homologene: 9068
Prdm2
Name: PR domain containing 2, with ZNF domain
Synonyms: Riz, Riz1, LOC381568, E330024L24Rik, 4833427P12Rik, KMT8
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 110593
HGNC: HGNC:9347
Homologene: 40822
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • A to C, chromosome 1 at 181,593,427 bp (GRCm38)
  • C to A, chromosome 2 at 60,330,725 bp (GRCm38)
  • T to C, chromosome 2 at 145,930,630 bp (GRCm38)
  • A to G, chromosome 2 at 174,120,237 bp (GRCm38)
  • G to A, chromosome 3 at 40,654,106 bp (GRCm38)
  • A to T, chromosome 3 at 49,755,057 bp (GRCm38)
  • G to T, chromosome 3 at 59,865,235 bp (GRCm38)
  • A to G, chromosome 3 at 98,334,634 bp (GRCm38)
  • A to G, chromosome 3 at 158,012,098 bp (GRCm38)
  • T to A, chromosome 4 at 49,514,338 bp (GRCm38)
  • A to C, chromosome 4 at 74,404,867 bp (GRCm38)
  • A to G, chromosome 4 at 108,513,232 bp (GRCm38)
  • G to T, chromosome 4 at 136,938,586 bp (GRCm38)
  • A to T, chromosome 4 at 143,134,991 bp (GRCm38)
  • T to C, chromosome 5 at 64,173,607 bp (GRCm38)
  • T to A, chromosome 6 at 71,139,826 bp (GRCm38)
  • C to T, chromosome 6 at 88,838,935 bp (GRCm38)
  • G to A, chromosome 6 at 115,782,796 bp (GRCm38)
  • C to G, chromosome 6 at 129,656,849 bp (GRCm38)
  • T to C, chromosome 7 at 18,480,162 bp (GRCm38)
  • C to T, chromosome 7 at 30,481,450 bp (GRCm38)
  • CTGTGTG to CTGTG, chromosome 7 at 142,211,999 bp (GRCm38)
  • A to T, chromosome 8 at 54,659,700 bp (GRCm38)
  • T to C, chromosome 8 at 69,820,731 bp (GRCm38)
  • G to A, chromosome 8 at 90,956,558 bp (GRCm38)
  • T to C, chromosome 8 at 91,251,245 bp (GRCm38)
  • A to T, chromosome 9 at 15,999,846 bp (GRCm38)
  • A to G, chromosome 9 at 20,500,450 bp (GRCm38)
  • G to A, chromosome 9 at 39,078,631 bp (GRCm38)
  • T to C, chromosome 9 at 70,820,864 bp (GRCm38)
  • A to G, chromosome 9 at 104,054,368 bp (GRCm38)
  • A to T, chromosome 9 at 113,655,106 bp (GRCm38)
  • A to G, chromosome 9 at 121,789,583 bp (GRCm38)
  • A to G, chromosome 10 at 77,912,633 bp (GRCm38)
  • A to T, chromosome 10 at 100,544,979 bp (GRCm38)
  • G to A, chromosome 10 at 120,038,664 bp (GRCm38)
  • A to G, chromosome 11 at 11,943,919 bp (GRCm38)
  • T to C, chromosome 11 at 44,456,424 bp (GRCm38)
  • G to T, chromosome 11 at 62,876,821 bp (GRCm38)
  • T to C, chromosome 11 at 96,158,762 bp (GRCm38)
  • T to C, chromosome 12 at 76,206,817 bp (GRCm38)
  • A to G, chromosome 12 at 110,658,371 bp (GRCm38)
  • A to G, chromosome 12 at 115,623,265 bp (GRCm38)
  • A to T, chromosome 13 at 23,099,285 bp (GRCm38)
  • A to G, chromosome 13 at 96,048,500 bp (GRCm38)
  • A to G, chromosome 13 at 113,617,218 bp (GRCm38)
  • A to G, chromosome 14 at 55,633,332 bp (GRCm38)
  • A to G, chromosome 14 at 63,542,406 bp (GRCm38)
  • A to T, chromosome 14 at 75,107,388 bp (GRCm38)
  • A to T, chromosome 14 at 103,054,858 bp (GRCm38)
  • T to C, chromosome 14 at 103,843,023 bp (GRCm38)
  • G to A, chromosome 15 at 3,969,011 bp (GRCm38)
  • A to G, chromosome 15 at 58,126,577 bp (GRCm38)
  • T to C, chromosome 16 at 17,499,740 bp (GRCm38)
  • T to C, chromosome 16 at 59,149,744 bp (GRCm38)
  • A to G, chromosome 17 at 14,946,197 bp (GRCm38)
  • T to C, chromosome 17 at 28,051,774 bp (GRCm38)
  • G to T, chromosome 17 at 38,335,450 bp (GRCm38)
  • A to G, chromosome 18 at 34,312,258 bp (GRCm38)
  • G to T, chromosome 19 at 45,557,920 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9547 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069343-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.