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Strain Name:
C57BL/6J-MtgxR9568Btlr/Mmmh
Stock Number:
069362-MU
Citation ID:
RRID:MMRRC_069362-MU
Other Names:
R9568 (G1)
Major Collection:

Strain Information

Trp53
Name: transformation related protein 53
Synonyms: p53, p44
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 22059
Homologene: 460
Slc12a1
Name: solute carrier family 12, member 1
Synonyms: Nkcc2, mBSC1, D630042G03Rik, urehr3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20495
Homologene: 286
Ptch1
Name: patched 1
Synonyms: Ptc, Patched 1, Ptc1, A230106A15Rik, wig
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 19206
HGNC: HGNC:9585
Homologene: 223
Slc4a7
Name: solute carrier family 4, sodium bicarbonate cotransporter, member 7
Synonyms: E430014N10Rik, NBC3, NBCn1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 218756
VEGA: 14
Homologene: 2680
Gch1
Name: GTP cyclohydrolase 1
Synonyms: GTPCH, GTP-CH
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 14528
VEGA: 14
HGNC: HGNC:4193
Homologene: 132
Lrrc2
Name: leucine rich repeat containing 2
Synonyms: 2400002D05Rik, 4933431K03Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 74249
Homologene: 23454
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • A to T, chromosome 1 at 45,391,838 bp (GRCm38)
  • T to A, chromosome 1 at 130,804,619 bp (GRCm38)
  • G to T, chromosome 1 at 150,101,091 bp (GRCm38)
  • C to A, chromosome 2 at 35,283,991 bp (GRCm38)
  • A to G, chromosome 2 at 40,679,215 bp (GRCm38)
  • G to A, chromosome 2 at 116,880,249 bp (GRCm38)
  • A to G, chromosome 2 at 125,190,298 bp (GRCm38)
  • A to T, chromosome 2 at 126,822,590 bp (GRCm38)
  • G to A, chromosome 2 at 173,228,001 bp (GRCm38)
  • T to C, chromosome 2 at 181,222,253 bp (GRCm38)
  • T to C, chromosome 3 at 20,266,533 bp (GRCm38)
  • T to G, chromosome 3 at 38,892,007 bp (GRCm38)
  • T to G, chromosome 3 at 103,736,966 bp (GRCm38)
  • T to A, chromosome 3 at 109,098,616 bp (GRCm38)
  • C to A, chromosome 4 at 26,340,468 bp (GRCm38)
  • G to T, chromosome 4 at 56,786,711 bp (GRCm38)
  • G to A, chromosome 4 at 88,722,010 bp (GRCm38)
  • G to T, chromosome 4 at 92,191,678 bp (GRCm38)
  • G to A, chromosome 4 at 116,554,775 bp (GRCm38)
  • G to T, chromosome 4 at 129,073,604 bp (GRCm38)
  • A to T, chromosome 5 at 114,857,105 bp (GRCm38)
  • T to C, chromosome 5 at 134,593,258 bp (GRCm38)
  • C to T, chromosome 5 at 137,735,132 bp (GRCm38)
  • T to C, chromosome 5 at 144,001,433 bp (GRCm38)
  • A to G, chromosome 6 at 30,640,061 bp (GRCm38)
  • T to A, chromosome 6 at 125,024,833 bp (GRCm38)
  • A to G, chromosome 7 at 6,474,335 bp (GRCm38)
  • A to G, chromosome 7 at 10,634,365 bp (GRCm38)
  • A to T, chromosome 7 at 28,315,263 bp (GRCm38)
  • A to T, chromosome 7 at 30,359,491 bp (GRCm38)
  • A to T, chromosome 7 at 75,125,680 bp (GRCm38)
  • A to G, chromosome 8 at 23,119,365 bp (GRCm38)
  • G to T, chromosome 8 at 61,372,551 bp (GRCm38)
  • G to A, chromosome 8 at 68,887,583 bp (GRCm38)
  • A to C, chromosome 8 at 105,253,571 bp (GRCm38)
  • G to A, chromosome 8 at 105,842,503 bp (GRCm38)
  • A to G, chromosome 8 at 122,584,910 bp (GRCm38)
  • A to T, chromosome 9 at 40,100,568 bp (GRCm38)
  • A to G, chromosome 9 at 70,035,064 bp (GRCm38)
  • A to T, chromosome 9 at 76,197,101 bp (GRCm38)
  • G to A, chromosome 9 at 96,688,891 bp (GRCm38)
  • G to A, chromosome 9 at 105,780,727 bp (GRCm38)
  • A to T, chromosome 9 at 107,300,394 bp (GRCm38)
  • A to G, chromosome 9 at 108,445,706 bp (GRCm38)
  • G to A, chromosome 9 at 110,970,160 bp (GRCm38)
  • A to G, chromosome 10 at 86,863,556 bp (GRCm38)
  • T to A, chromosome 10 at 130,179,945 bp (GRCm38)
  • T to A, chromosome 11 at 26,468,672 bp (GRCm38)
  • T to C, chromosome 11 at 34,708,811 bp (GRCm38)
  • T to A, chromosome 11 at 69,164,010 bp (GRCm38)
  • C to A, chromosome 11 at 69,587,566 bp (GRCm38)
  • A to G, chromosome 11 at 102,356,854 bp (GRCm38)
  • G to T, chromosome 12 at 35,948,350 bp (GRCm38)
  • T to C, chromosome 12 at 113,456,724 bp (GRCm38)
  • T to G, chromosome 12 at 113,544,400 bp (GRCm38)
  • G to T, chromosome 13 at 21,958,727 bp (GRCm38)
  • A to T, chromosome 13 at 58,009,312 bp (GRCm38)
  • A to G, chromosome 13 at 63,542,173 bp (GRCm38)
  • T to A, chromosome 13 at 100,219,830 bp (GRCm38)
  • T to C, chromosome 13 at 100,223,313 bp (GRCm38)
  • A to G, chromosome 14 at 14,796,073 bp (GRCm38)
  • A to G, chromosome 14 at 47,189,180 bp (GRCm38)
  • A to G, chromosome 15 at 48,287,546 bp (GRCm38)
  • A to T, chromosome 15 at 89,160,957 bp (GRCm38)
  • T to A, chromosome 15 at 98,722,938 bp (GRCm38)
  • T to G, chromosome 15 at 99,278,690 bp (GRCm38)
  • C to A, chromosome 15 at 102,445,519 bp (GRCm38)
  • A to C, chromosome 16 at 17,567,436 bp (GRCm38)
  • A to G, chromosome 16 at 18,084,755 bp (GRCm38)
  • C to A, chromosome 16 at 59,035,850 bp (GRCm38)
  • G to T, chromosome 16 at 91,852,894 bp (GRCm38)
  • A to T, chromosome 17 at 21,314,515 bp (GRCm38)
  • G to A, chromosome 17 at 46,520,118 bp (GRCm38)
  • A to T, chromosome 17 at 71,087,481 bp (GRCm38)
  • A to G, chromosome 19 at 4,811,436 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9568 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069362-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.