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Strain Name:
C57BL/6J-MtgxR9570Btlr/Mmmh
Stock Number:
069363-MU
Citation ID:
RRID:MMRRC_069363-MU
Other Names:
R9570 (G1)
Major Collection:

Strain Information

Smarca4
Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Synonyms: SNF2beta, Brg1, SW1/SNF, b2b692Clo, b2b508.1Clo
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20586
Homologene: 135927
Dgat2
Name: diacylglycerol O-acyltransferase 2
Synonyms: 0610010B06Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 67800
Homologene: 23580
Gtf2i
Name: general transcription factor II I
Synonyms: TFII-I, BAP-135, 6030441I21Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14886
HGNC: HGNC:4659
Homologene: 7748
Fryl
Name: FRY like transcription coactivator
Synonyms: 2310004H21Rik, 2510002A14Rik, 9030227G01Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 72313
Homologene: 103956
Rlf
Name: rearranged L-myc fusion sequence
Synonyms: 9230110M18Rik, MommeD8
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 109263
Homologene: 8243
Ankrd17
Name: ankyrin repeat domain 17
Synonyms: Gtar, 4933425K22Rik, A130069E23Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 81702
Homologene: 82403
Aplf
Name: aprataxin and PNKP like factor
Synonyms: 2010301N04Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 72103
Homologene: 49753
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • C to A, chromosome 1 at 152,554,331 bp (GRCm38)
  • A to G, chromosome 1 at 164,379,392 bp (GRCm38)
  • G to T, chromosome 2 at 25,408,300 bp (GRCm38)
  • T to C, chromosome 2 at 74,682,468 bp (GRCm38)
  • A to G, chromosome 2 at 76,046,813 bp (GRCm38)
  • C to T, chromosome 2 at 82,258,500 bp (GRCm38)
  • G to A, chromosome 2 at 120,704,233 bp (GRCm38)
  • G to A, chromosome 4 at 58,832,796 bp (GRCm38)
  • A to G, chromosome 4 at 86,828,971 bp (GRCm38)
  • A to G, chromosome 4 at 121,149,890 bp (GRCm38)
  • C to T, chromosome 4 at 134,542,744 bp (GRCm38)
  • A to T, chromosome 4 at 143,417,944 bp (GRCm38)
  • C to T, chromosome 5 at 5,546,382 bp (GRCm38)
  • T to C, chromosome 5 at 30,622,089 bp (GRCm38)
  • T to A, chromosome 5 at 31,243,928 bp (GRCm38)
  • T to C, chromosome 5 at 73,022,155 bp (GRCm38)
  • G to T, chromosome 5 at 90,253,677 bp (GRCm38)
  • G to A, chromosome 5 at 113,865,755 bp (GRCm38)
  • G to A, chromosome 5 at 134,265,773 bp (GRCm38)
  • T to A, chromosome 5 at 137,836,080 bp (GRCm38)
  • C to T, chromosome 5 at 147,372,614 bp (GRCm38)
  • T to C, chromosome 6 at 48,905,838 bp (GRCm38)
  • T to C, chromosome 6 at 66,637,734 bp (GRCm38)
  • T to A, chromosome 6 at 87,663,799 bp (GRCm38)
  • T to A, chromosome 6 at 116,405,678 bp (GRCm38)
  • G to T, chromosome 7 at 24,593,811 bp (GRCm38)
  • T to C, chromosome 7 at 34,192,976 bp (GRCm38)
  • A to T, chromosome 7 at 41,650,582 bp (GRCm38)
  • A to G, chromosome 7 at 43,828,543 bp (GRCm38)
  • C to T, chromosome 7 at 45,615,170 bp (GRCm38)
  • A to G, chromosome 7 at 79,826,913 bp (GRCm38)
  • C to T, chromosome 7 at 99,169,719 bp (GRCm38)
  • A to G, chromosome 7 at 103,881,649 bp (GRCm38)
  • A to G, chromosome 7 at 108,881,297 bp (GRCm38)
  • G to A, chromosome 8 at 33,577,281 bp (GRCm38)
  • T to A, chromosome 8 at 33,762,048 bp (GRCm38)
  • C to T, chromosome 8 at 40,824,650 bp (GRCm38)
  • T to A, chromosome 8 at 71,367,829 bp (GRCm38)
  • A to G, chromosome 8 at 71,406,780 bp (GRCm38)
  • A to C, chromosome 8 at 77,667,332 bp (GRCm38)
  • T to C, chromosome 8 at 83,668,832 bp (GRCm38)
  • T to A, chromosome 9 at 9,148,208 bp (GRCm38)
  • A to G, chromosome 9 at 21,669,553 bp (GRCm38)
  • T to C, chromosome 9 at 110,398,149 bp (GRCm38)
  • A to T, chromosome 9 at 121,784,216 bp (GRCm38)
  • A to G, chromosome 10 at 20,000,568 bp (GRCm38)
  • T to C, chromosome 10 at 78,520,250 bp (GRCm38)
  • A to G, chromosome 10 at 80,525,988 bp (GRCm38)
  • A to T, chromosome 10 at 86,713,734 bp (GRCm38)
  • A to G, chromosome 11 at 5,829,713 bp (GRCm38)
  • T to A, chromosome 11 at 8,890,697 bp (GRCm38)
  • A to G, chromosome 11 at 60,185,742 bp (GRCm38)
  • C to T, chromosome 11 at 116,659,757 bp (GRCm38)
  • G to A, chromosome 12 at 85,094,992 bp (GRCm38)
  • A to G, chromosome 12 at 87,895,764 bp (GRCm38)
  • A to T, chromosome 12 at 98,815,984 bp (GRCm38)
  • A to T, chromosome 12 at 116,000,174 bp (GRCm38)
  • T to C, chromosome 13 at 22,475,066 bp (GRCm38)
  • T to C, chromosome 13 at 55,805,856 bp (GRCm38)
  • T to C, chromosome 13 at 100,223,313 bp (GRCm38)
  • T to A, chromosome 14 at 31,142,681 bp (GRCm38)
  • T to C, chromosome 14 at 34,092,552 bp (GRCm38)
  • A to C, chromosome 14 at 56,231,585 bp (GRCm38)
  • T to C, chromosome 14 at 62,360,529 bp (GRCm38)
  • C to T, chromosome 15 at 77,640,571 bp (GRCm38)
  • T to C, chromosome 16 at 13,819,384 bp (GRCm38)
  • A to T, chromosome 16 at 16,260,414 bp (GRCm38)
  • T to A, chromosome 16 at 21,644,203 bp (GRCm38)
  • A to T, chromosome 16 at 32,750,877 bp (GRCm38)
  • A to T, chromosome 16 at 45,095,086 bp (GRCm38)
  • A to T, chromosome 16 at 45,560,342 bp (GRCm38)
  • A to G, chromosome 16 at 56,750,450 bp (GRCm38)
  • T to A, chromosome 16 at 57,628,176 bp (GRCm38)
  • A to T, chromosome 18 at 42,139,555 bp (GRCm38)
  • T to C, chromosome 18 at 44,445,858 bp (GRCm38)
  • T to A, chromosome 19 at 6,255,719 bp (GRCm38)
  • A to T, chromosome 19 at 9,992,967 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9570 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069363-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.