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Strain Name:
C57BL/6J-MtgxR9657Btlr/Mmmh
Stock Number:
069450-MU
Citation ID:
RRID:MMRRC_069450-MU
Other Names:
R9657 (G1)
Major Collection:

Strain Information

Hdgfl2
Name: HDGF like 2
Synonyms: HRP-2, Hdgfrp2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 15193
VEGA: 17
Homologene: 7355
Ptprz1
Name: protein tyrosine phosphatase receptor type Z, polypeptide 1
Synonyms: DSD-1-PG, phosphacan, PTPzeta, PTPbeta, Rptpbeta, Ptpz, Ptprz, RPTPz
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 19283
HGNC: HGNC:9685
Homologene: 2136
Rad54l2
Name: RAD54 like 2 (S. cerevisiae)
Synonyms: Arip4, G630026H09Rik, Srisnf2l
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 81000
Homologene: 56698
Dock1
Name: dedicator of cytokinesis 1
Synonyms: D630004B07Rik, 9130006G06Rik, Dock180, b2b3190Clo
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 330662
HGNC: HGNC:2987
Homologene: 55575
Rapgef2
Name: Rap guanine nucleotide exchange factor (GEF) 2
Synonyms: 5830453M24Rik, Pdzgef1, RA-GEF-1, CNRasGEF, nRapGEP
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 76089
Homologene: 35477
Ppp1r1c
Name: protein phosphatase 1, regulatory inhibitor subunit 1C
Synonyms: 4930565M23Rik, 4930579P15Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 75276
Homologene: 18170
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • C to A, chromosome 1 at 34,473,392 bp (GRCm38)
  • T to C, chromosome 1 at 133,728,740 bp (GRCm38)
  • C to T, chromosome 1 at 162,639,660 bp (GRCm38)
  • T to C, chromosome 1 at 164,306,454 bp (GRCm38)
  • T to C, chromosome 1 at 166,386,743 bp (GRCm38)
  • T to C, chromosome 1 at 181,510,539 bp (GRCm38)
  • T to C, chromosome 2 at 10,102,875 bp (GRCm38)
  • T to C, chromosome 2 at 65,735,688 bp (GRCm38)
  • C to T, chromosome 2 at 76,885,013 bp (GRCm38)
  • A to G, chromosome 2 at 79,808,374 bp (GRCm38)
  • A to G, chromosome 2 at 84,737,724 bp (GRCm38)
  • T to C, chromosome 2 at 87,693,777 bp (GRCm38)
  • A to C, chromosome 2 at 87,990,966 bp (GRCm38)
  • T to A, chromosome 2 at 94,406,665 bp (GRCm38)
  • A to G, chromosome 2 at 114,197,285 bp (GRCm38)
  • A to G, chromosome 2 at 120,304,657 bp (GRCm38)
  • G to A, chromosome 2 at 122,080,300 bp (GRCm38)
  • A to G, chromosome 2 at 155,614,043 bp (GRCm38)
  • T to C, chromosome 2 at 165,123,795 bp (GRCm38)
  • G to T, chromosome 3 at 9,704,731 bp (GRCm38)
  • G to A, chromosome 3 at 19,674,507 bp (GRCm38)
  • A to G, chromosome 3 at 54,383,399 bp (GRCm38)
  • A to T, chromosome 3 at 79,091,884 bp (GRCm38)
  • A to G, chromosome 3 at 89,447,749 bp (GRCm38)
  • A to G, chromosome 3 at 132,715,690 bp (GRCm38)
  • A to G, chromosome 4 at 11,544,898 bp (GRCm38)
  • A to C, chromosome 4 at 108,027,034 bp (GRCm38)
  • A to T, chromosome 4 at 108,718,532 bp (GRCm38)
  • C to A, chromosome 4 at 150,614,933 bp (GRCm38)
  • C to T, chromosome 5 at 134,504,762 bp (GRCm38)
  • T to C, chromosome 5 at 145,450,169 bp (GRCm38)
  • T to G, chromosome 6 at 23,042,378 bp (GRCm38)
  • C to G, chromosome 6 at 90,270,002 bp (GRCm38)
  • A to G, chromosome 7 at 112,322,599 bp (GRCm38)
  • A to G, chromosome 7 at 125,842,784 bp (GRCm38)
  • T to C, chromosome 7 at 127,887,066 bp (GRCm38)
  • T to C, chromosome 7 at 134,737,700 bp (GRCm38)
  • T to C, chromosome 8 at 70,177,610 bp (GRCm38)
  • C to T, chromosome 8 at 75,117,540 bp (GRCm38)
  • T to C, chromosome 8 at 84,260,455 bp (GRCm38)
  • A to T, chromosome 8 at 105,279,009 bp (GRCm38)
  • T to C, chromosome 9 at 89,199,546 bp (GRCm38)
  • T to A, chromosome 9 at 104,075,836 bp (GRCm38)
  • C to A, chromosome 9 at 106,704,173 bp (GRCm38)
  • T to A, chromosome 10 at 24,252,587 bp (GRCm38)
  • A to G, chromosome 10 at 100,515,141 bp (GRCm38)
  • T to A, chromosome 11 at 9,293,379 bp (GRCm38)
  • A to T, chromosome 11 at 30,838,980 bp (GRCm38)
  • A to G, chromosome 11 at 102,400,752 bp (GRCm38)
  • G to A, chromosome 12 at 103,689,791 bp (GRCm38)
  • A to C, chromosome 12 at 112,036,390 bp (GRCm38)
  • A to G, chromosome 13 at 43,403,679 bp (GRCm38)
  • A to T, chromosome 14 at 26,429,858 bp (GRCm38)
  • T to C, chromosome 14 at 50,896,660 bp (GRCm38)
  • A to C, chromosome 14 at 118,788,369 bp (GRCm38)
  • A to C, chromosome 15 at 9,280,047 bp (GRCm38)
  • A to T, chromosome 15 at 28,409,943 bp (GRCm38)
  • A to G, chromosome 15 at 76,361,276 bp (GRCm38)
  • T to A, chromosome 16 at 56,123,932 bp (GRCm38)
  • T to C, chromosome 16 at 65,839,457 bp (GRCm38)
  • G to A, chromosome 16 at 96,173,753 bp (GRCm38)
  • C to T, chromosome 17 at 6,069,383 bp (GRCm38)
  • A to G, chromosome 17 at 23,291,716 bp (GRCm38)
  • T to C, chromosome 17 at 56,098,978 bp (GRCm38)
  • T to C, chromosome 17 at 81,647,815 bp (GRCm38)
  • A to G, chromosome 18 at 63,924,847 bp (GRCm38)
  • A to G, chromosome 19 at 56,363,945 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9657 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069450-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.