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Strain Name:
C57BL/6J-MtgxR9674Btlr/Mmmh
Stock Number:
069467-MU
Citation ID:
RRID:MMRRC_069467-MU
Other Names:
R9674 (G1)
Major Collection:

Strain Information

Ighm
Name: immunoglobulin heavy constant mu
Synonyms: Ig mu, Igh6, Igh-M, muH, IgM
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 16019
HGNC: HGNC:5541
Cadps
Name: Ca2+-dependent secretion activator
Synonyms: CAPS1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 27062
VEGA: 14
HGNC: HGNC:1426
Homologene: 2755
Grm1
Name: glutamate receptor, metabotropic 1
Synonyms: mGluR1, Grm1, Gprc1a, nmf373, rcw, 4930455H15Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 14816
HGNC: HGNC:4593
Homologene: 649
Cyp19a1
Name: cytochrome P450, family 19, subfamily a, polypeptide 1
Synonyms: Int-5, Int5, aromatase, Ar, Cyp19, ArKO, p450arom
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 13075
VEGA: 9
HGNC: HGNC:2594
Homologene: 30955
Dlg1
Name: discs large MAGUK scaffold protein 1
Synonyms: B130052P05Rik, SAP97, Dlgh1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 13383
HGNC: HGNC:2900
Homologene: 20869
Xrn1
Name: 5'-3' exoribonuclease 1
Synonyms: mXrn1, Dhm2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 24127
Homologene: 5894
Prkdc
Name: protein kinase, DNA activated, catalytic polypeptide
Synonyms: DNA-PKcs, slip, DNAPDcs, XRCC7, DNA-PK, DOXNPH, dxnph
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 19090
HGNC: HGNC:9413
Homologene: 5037
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • T to C, chromosome 1 at 36,771,069 bp (GRCm38)
  • C to T, chromosome 1 at 38,209,783 bp (GRCm38)
  • T to A, chromosome 1 at 97,119,840 bp (GRCm38)
  • A to T, chromosome 2 at 25,236,318 bp (GRCm38)
  • T to A, chromosome 2 at 25,794,370 bp (GRCm38)
  • G to A, chromosome 2 at 26,471,296 bp (GRCm38)
  • A to G, chromosome 2 at 45,001,713 bp (GRCm38)
  • T to C, chromosome 2 at 62,764,716 bp (GRCm38)
  • C to T, chromosome 2 at 71,546,152 bp (GRCm38)
  • T to G, chromosome 2 at 85,648,249 bp (GRCm38)
  • G to T, chromosome 2 at 93,677,513 bp (GRCm38)
  • C to T, chromosome 2 at 180,198,474 bp (GRCm38)
  • A to G, chromosome 3 at 38,452,425 bp (GRCm38)
  • A to T, chromosome 3 at 56,058,762 bp (GRCm38)
  • A to T, chromosome 3 at 60,007,051 bp (GRCm38)
  • T to C, chromosome 3 at 92,777,806 bp (GRCm38)
  • T to A, chromosome 4 at 44,931,418 bp (GRCm38)
  • T to A, chromosome 4 at 65,542,726 bp (GRCm38)
  • T to C, chromosome 4 at 115,328,959 bp (GRCm38)
  • C to T, chromosome 4 at 120,969,548 bp (GRCm38)
  • T to A, chromosome 4 at 143,793,592 bp (GRCm38)
  • A to G, chromosome 5 at 5,647,021 bp (GRCm38)
  • A to G, chromosome 6 at 37,050,222 bp (GRCm38)
  • C to T, chromosome 6 at 60,971,088 bp (GRCm38)
  • T to C, chromosome 6 at 70,335,868 bp (GRCm38)
  • T to C, chromosome 6 at 118,153,869 bp (GRCm38)
  • A to T, chromosome 7 at 44,462,428 bp (GRCm38)
  • A to T, chromosome 7 at 45,333,387 bp (GRCm38)
  • C to T, chromosome 7 at 105,714,222 bp (GRCm38)
  • A to T, chromosome 7 at 108,323,064 bp (GRCm38)
  • A to G, chromosome 7 at 120,475,445 bp (GRCm38)
  • T to A, chromosome 7 at 126,124,528 bp (GRCm38)
  • T to G, chromosome 7 at 128,778,463 bp (GRCm38)
  • T to C, chromosome 8 at 12,827,525 bp (GRCm38)
  • T to A, chromosome 8 at 24,950,998 bp (GRCm38)
  • C to T, chromosome 8 at 82,343,309 bp (GRCm38)
  • A to G, chromosome 8 at 119,445,760 bp (GRCm38)
  • A to T, chromosome 9 at 54,166,857 bp (GRCm38)
  • A to T, chromosome 9 at 95,973,592 bp (GRCm38)
  • A to T, chromosome 9 at 95,973,594 bp (GRCm38)
  • A to T, chromosome 9 at 107,689,748 bp (GRCm38)
  • A to G, chromosome 9 at 120,494,199 bp (GRCm38)
  • A to G, chromosome 10 at 10,733,284 bp (GRCm38)
  • A to T, chromosome 10 at 30,834,417 bp (GRCm38)
  • T to A, chromosome 10 at 69,988,719 bp (GRCm38)
  • C to T, chromosome 10 at 82,284,196 bp (GRCm38)
  • T to G, chromosome 11 at 59,465,024 bp (GRCm38)
  • T to C, chromosome 11 at 69,779,147 bp (GRCm38)
  • T to C, chromosome 11 at 82,755,012 bp (GRCm38)
  • T to A, chromosome 11 at 101,276,048 bp (GRCm38)
  • A to G, chromosome 12 at 80,489,598 bp (GRCm38)
  • T to A, chromosome 12 at 100,466,294 bp (GRCm38)
  • G to T, chromosome 12 at 109,592,590 bp (GRCm38)
  • T to A, chromosome 12 at 113,421,519 bp (GRCm38)
  • G to T, chromosome 12 at 115,312,227 bp (GRCm38)
  • A to G, chromosome 13 at 23,574,688 bp (GRCm38)
  • T to C, chromosome 13 at 24,926,055 bp (GRCm38)
  • G to A, chromosome 13 at 104,426,940 bp (GRCm38)
  • A to G, chromosome 14 at 12,454,291 bp (GRCm38)
  • C to T, chromosome 14 at 30,773,894 bp (GRCm38)
  • G to C, chromosome 14 at 47,684,756 bp (GRCm38)
  • A to T, chromosome 14 at 101,840,904 bp (GRCm38)
  • C to G, chromosome 15 at 12,890,084 bp (GRCm38)
  • T to A, chromosome 15 at 35,607,234 bp (GRCm38)
  • C to A, chromosome 15 at 54,952,739 bp (GRCm38)
  • G to A, chromosome 15 at 77,517,147 bp (GRCm38)
  • T to A, chromosome 15 at 79,365,324 bp (GRCm38)
  • A to G, chromosome 15 at 80,380,428 bp (GRCm38)
  • T to C, chromosome 16 at 7,353,021 bp (GRCm38)
  • T to A, chromosome 16 at 9,653,401 bp (GRCm38)
  • T to A, chromosome 16 at 15,715,955 bp (GRCm38)
  • T to C, chromosome 16 at 17,574,344 bp (GRCm38)
  • T to C, chromosome 16 at 31,791,762 bp (GRCm38)
  • A to G, chromosome 16 at 96,640,836 bp (GRCm38)
  • G to T, chromosome 17 at 30,779,138 bp (GRCm38)
  • T to A, chromosome 17 at 32,627,867 bp (GRCm38)
  • A to G, chromosome 17 at 65,833,132 bp (GRCm38)
  • T to A, chromosome 18 at 43,571,896 bp (GRCm38)
  • G to A, chromosome 18 at 86,473,702 bp (GRCm38)
  • T to A, chromosome 19 at 37,701,278 bp (GRCm38)
  • T to A, chromosome 19 at 56,933,763 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9674 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069467-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.