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Strain Name:
C57BL/6J-MtgxR9682Btlr/Mmmh
Stock Number:
069475-MU
Citation ID:
RRID:MMRRC_069475-MU
Other Names:
R9682 (G1)
Major Collection:

Strain Information

Dicer1
Name: dicer 1, ribonuclease type III
Synonyms: 1110006F08Rik, D12Ertd7e, Dicer1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 192119
VEGA: 12
Homologene: 13251
Ccr2
Name: C-C motif chemokine receptor 2
Synonyms: CC-CKR-2, CKR2B, CKR2A, CCR2B, CCR2A, CKR2, Cmkbr2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 12772
HGNC: HGNC:1603
Homologene: 537
Sh3gl2
Name: SH3-domain GRB2-like 2
Synonyms: EEN-B1, endophilin I, Sh3d2a, 9530001L19Rik, B930049H17Rik, endophilin A1, EEN1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 20404
Homologene: 20652
Sema3b
Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B
Synonyms: SemA, semaV, sema5, SemA, LUCA-1, Semaa
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20347
Homologene: 20972
Slc6a12
Name: solute carrier family 6 (neurotransmitter transporter, betaine/GABA), member 12
Synonyms: Gabt2, BGT1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 14411
Homologene: 128225
Lyst
Name: lysosomal trafficking regulator
Synonyms: D13Sfk13
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17101
VEGA: 13
HGNC: HGNC:1968
Homologene: 61
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • T to C, chromosome 1 at 60,765,346 bp (GRCm38)
  • A to T, chromosome 1 at 117,948,094 bp (GRCm38)
  • G to T, chromosome 2 at 11,486,247 bp (GRCm38)
  • A to G, chromosome 2 at 68,366,105 bp (GRCm38)
  • T to A, chromosome 2 at 89,023,952 bp (GRCm38)
  • T to A, chromosome 2 at 111,420,392 bp (GRCm38)
  • T to C, chromosome 2 at 129,033,609 bp (GRCm38)
  • C to T, chromosome 3 at 30,689,274 bp (GRCm38)
  • C to T, chromosome 3 at 36,082,119 bp (GRCm38)
  • T to A, chromosome 3 at 90,144,162 bp (GRCm38)
  • A to G, chromosome 3 at 103,993,898 bp (GRCm38)
  • G to T, chromosome 3 at 158,177,317 bp (GRCm38)
  • A to G, chromosome 4 at 85,377,511 bp (GRCm38)
  • C to A, chromosome 4 at 131,928,509 bp (GRCm38)
  • A to G, chromosome 4 at 133,248,108 bp (GRCm38)
  • A to T, chromosome 5 at 8,702,507 bp (GRCm38)
  • G to A, chromosome 5 at 32,830,679 bp (GRCm38)
  • T to A, chromosome 5 at 71,641,072 bp (GRCm38)
  • A to T, chromosome 5 at 87,139,663 bp (GRCm38)
  • T to C, chromosome 5 at 104,478,924 bp (GRCm38)
  • A to T, chromosome 5 at 110,103,611 bp (GRCm38)
  • A to T, chromosome 5 at 123,511,453 bp (GRCm38)
  • A to T, chromosome 5 at 136,308,262 bp (GRCm38)
  • A to T, chromosome 5 at 145,715,516 bp (GRCm38)
  • A to T, chromosome 5 at 150,043,099 bp (GRCm38)
  • G to A, chromosome 6 at 121,363,745 bp (GRCm38)
  • C to T, chromosome 7 at 7,296,798 bp (GRCm38)
  • A to T, chromosome 7 at 27,158,186 bp (GRCm38)
  • T to G, chromosome 7 at 82,643,673 bp (GRCm38)
  • G to T, chromosome 7 at 104,580,227 bp (GRCm38)
  • A to G, chromosome 7 at 126,862,849 bp (GRCm38)
  • A to G, chromosome 7 at 138,894,716 bp (GRCm38)
  • T to A, chromosome 8 at 3,707,713 bp (GRCm38)
  • A to T, chromosome 8 at 22,351,477 bp (GRCm38)
  • C to T, chromosome 9 at 18,642,578 bp (GRCm38)
  • T to C, chromosome 9 at 37,540,475 bp (GRCm38)
  • G to A, chromosome 9 at 39,078,578 bp (GRCm38)
  • G to A, chromosome 9 at 48,392,948 bp (GRCm38)
  • A to G, chromosome 9 at 65,184,050 bp (GRCm38)
  • T to A, chromosome 9 at 107,603,814 bp (GRCm38)
  • A to G, chromosome 9 at 122,986,647 bp (GRCm38)
  • A to G, chromosome 9 at 124,106,139 bp (GRCm38)
  • A to T, chromosome 10 at 14,440,384 bp (GRCm38)
  • A to G, chromosome 10 at 79,819,205 bp (GRCm38)
  • T to C, chromosome 10 at 80,535,396 bp (GRCm38)
  • C to T, chromosome 10 at 81,273,811 bp (GRCm38)
  • T to C, chromosome 10 at 98,979,800 bp (GRCm38)
  • C to T, chromosome 11 at 73,395,199 bp (GRCm38)
  • A to C, chromosome 11 at 96,820,118 bp (GRCm38)
  • A to G, chromosome 11 at 100,894,767 bp (GRCm38)
  • A to G, chromosome 12 at 104,706,225 bp (GRCm38)
  • T to C, chromosome 12 at 113,131,764 bp (GRCm38)
  • T to A, chromosome 12 at 118,932,593 bp (GRCm38)
  • A to G, chromosome 13 at 13,656,941 bp (GRCm38)
  • A to G, chromosome 14 at 42,311,438 bp (GRCm38)
  • A to G, chromosome 15 at 58,550,264 bp (GRCm38)
  • T to C, chromosome 16 at 16,484,338 bp (GRCm38)
  • T to C, chromosome 16 at 16,829,117 bp (GRCm38)
  • A to G, chromosome 16 at 33,720,928 bp (GRCm38)
  • A to T, chromosome 16 at 57,290,817 bp (GRCm38)
  • G to A, chromosome 17 at 56,769,770 bp (GRCm38)
  • T to G, chromosome 17 at 58,950,267 bp (GRCm38)
  • A to T, chromosome 17 at 71,875,063 bp (GRCm38)
  • A to G, chromosome 18 at 43,358,248 bp (GRCm38)
  • A to T, chromosome 19 at 24,378,954 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9682 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069475-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.