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Strain Name:
C57BL/6J-MtgxR9686Btlr/Mmmh
Stock Number:
069479-MU
Citation ID:
RRID:MMRRC_069479-MU
Other Names:
R9686 (G1)
Major Collection:

Strain Information

Ank2
Name: ankyrin 2, brain
Synonyms: ankyrin B, Ank-2, Ankyrin-2, Ankyrin-B, Gm4392
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 109676
HGNC: HGNC:493
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Spen
Name: spen family transcription repressor
Synonyms: Mint
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 56381
Homologene: 124461
Gng12
Name: guanine nucleotide binding protein (G protein), gamma 12
Synonyms: 2010305F15Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 14701
Homologene: 32427
Rbm47
Name: RNA binding motif protein 47
Synonyms: 9530077J19Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 245945
Homologene: 36932
Ube2o
Name: ubiquitin-conjugating enzyme E2O
Synonyms: B230113M03Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217342
Homologene: 11113
Akap13
Name: A kinase anchor protein 13
Synonyms: AKAP-Lbc, PROTO-LBC, PROTO-LB, Ht31, 5730522G15Rik, 1700026G02Rik, 5830460E08Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 75547
HGNC: HGNC:371
Homologene: 4903
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • A to T, chromosome 1 at 36,266,458 bp (GRCm38)
  • T to A, chromosome 1 at 65,252,456 bp (GRCm38)
  • A to T, chromosome 1 at 82,497,241 bp (GRCm38)
  • G to A, chromosome 1 at 150,737,605 bp (GRCm38)
  • G to A, chromosome 1 at 171,357,809 bp (GRCm38)
  • A to G, chromosome 1 at 173,225,413 bp (GRCm38)
  • A to G, chromosome 1 at 175,632,220 bp (GRCm38)
  • A to C, chromosome 2 at 25,313,510 bp (GRCm38)
  • T to C, chromosome 2 at 62,573,513 bp (GRCm38)
  • T to A, chromosome 2 at 85,579,996 bp (GRCm38)
  • A to T, chromosome 2 at 86,046,991 bp (GRCm38)
  • T to C, chromosome 3 at 13,776,827 bp (GRCm38)
  • T to A, chromosome 3 at 126,946,901 bp (GRCm38)
  • A to G, chromosome 4 at 83,549,682 bp (GRCm38)
  • A to G, chromosome 4 at 123,483,698 bp (GRCm38)
  • A to T, chromosome 4 at 129,155,478 bp (GRCm38)
  • G to T, chromosome 4 at 136,938,586 bp (GRCm38)
  • A to G, chromosome 4 at 141,472,635 bp (GRCm38)
  • G to A, chromosome 5 at 38,674,114 bp (GRCm38)
  • A to T, chromosome 5 at 66,022,626 bp (GRCm38)
  • T to C, chromosome 6 at 29,456,435 bp (GRCm38)
  • T to C, chromosome 6 at 67,017,545 bp (GRCm38)
  • C to T, chromosome 7 at 17,158,198 bp (GRCm38)
  • A to G, chromosome 7 at 20,510,428 bp (GRCm38)
  • A to G, chromosome 7 at 30,661,973 bp (GRCm38)
  • A to G, chromosome 7 at 75,586,336 bp (GRCm38)
  • A to G, chromosome 7 at 81,805,850 bp (GRCm38)
  • G to A, chromosome 7 at 84,940,876 bp (GRCm38)
  • A to G, chromosome 8 at 26,979,016 bp (GRCm38)
  • T to A, chromosome 8 at 110,109,931 bp (GRCm38)
  • C to A, chromosome 8 at 125,866,027 bp (GRCm38)
  • A to G, chromosome 9 at 21,206,266 bp (GRCm38)
  • G to A, chromosome 10 at 23,993,411 bp (GRCm38)
  • T to A, chromosome 10 at 39,867,388 bp (GRCm38)
  • A to T, chromosome 10 at 116,368,789 bp (GRCm38)
  • T to A, chromosome 10 at 127,050,316 bp (GRCm38)
  • A to G, chromosome 10 at 128,480,906 bp (GRCm38)
  • T to A, chromosome 11 at 23,474,351 bp (GRCm38)
  • A to T, chromosome 11 at 82,957,175 bp (GRCm38)
  • A to G, chromosome 11 at 94,357,041 bp (GRCm38)
  • A to T, chromosome 11 at 116,543,953 bp (GRCm38)
  • G to T, chromosome 11 at 118,088,222 bp (GRCm38)
  • A to T, chromosome 12 at 110,855,498 bp (GRCm38)
  • A to G, chromosome 13 at 46,525,450 bp (GRCm38)
  • T to C, chromosome 13 at 49,557,353 bp (GRCm38)
  • G to A, chromosome 14 at 56,552,591 bp (GRCm38)
  • G to A, chromosome 14 at 60,751,659 bp (GRCm38)
  • G to T, chromosome 14 at 65,975,005 bp (GRCm38)
  • A to G, chromosome 14 at 69,796,556 bp (GRCm38)
  • C to T, chromosome 15 at 4,945,123 bp (GRCm38)
  • A to G, chromosome 15 at 25,985,055 bp (GRCm38)
  • A to G, chromosome 15 at 45,109,104 bp (GRCm38)
  • G to A, chromosome 15 at 101,364,400 bp (GRCm38)
  • A to G, chromosome 16 at 23,496,137 bp (GRCm38)
  • A to G, chromosome 16 at 45,250,509 bp (GRCm38)
  • T to C, chromosome 16 at 45,580,214 bp (GRCm38)
  • T to C, chromosome 16 at 58,760,763 bp (GRCm38)
  • C to T, chromosome 17 at 21,463,609 bp (GRCm38)
  • A to G, chromosome 17 at 33,268,879 bp (GRCm38)
  • G to T, chromosome 17 at 66,519,518 bp (GRCm38)
  • A to T, chromosome 17 at 94,877,460 bp (GRCm38)
  • C to A, chromosome 18 at 14,799,158 bp (GRCm38)
  • A to C, chromosome 18 at 37,309,890 bp (GRCm38)
  • A to G, chromosome 18 at 58,838,021 bp (GRCm38)
  • T to C, chromosome 19 at 40,393,510 bp (GRCm38)
  • A to G, chromosome 19 at 61,225,191 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9686 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069479-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.