Strain Detail Sheet

Strain Name:
C57BL/6N-Prss29em2Tesp/Mmmh
Stock Number:
069570-MU
Citation ID:
RRID:MMRRC_069570-MU
Other Names:
Prss29-Cre

Strain Information

Gene Details

Prss29em2Tesp
Name: protease, serine 29; endonuclease-mediated mutation 2, Thomas E Spencer
Type: Allele
Species: Multi-species
Chromosome: 17
Prss29
Name: serine protease 29
Synonyms: mISP-2, Isp2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 114662
VEGA: 17
Homologene: 70877
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Genetic Alterations
Codon-optimized Cre, EGFP, self-cleaving peptide 2A, and a floxed neomycin cassette were knocked into the 3’ end of the Prss29 gene after exon 6, downstream of an internal ribosome entry site.
ES Cell Line
JM8.F6 derived from C57BL/6N

Strain Description

Phenotype
IRES-Codon optimized Cre and EGFP were knocked into the 3’ end of the Prss29 locus. Cre is expressed specifically in the glandular epithelium of the uterus during pregnancy.
MeSH Terms
  • Pregnancy
  • Mice
  • Animals
  • Female
  • Placenta/metabolism
  • Uterus/physiology
  • Endometrium/metabolism
  • Embryo Implantation/genetics
  • Mammals
Strain Development

CRISPR/Cas9 was used on C57BL/6N-derived JM8.F6 ESCs to knock in the described genetic alteration. Subsequent breeding used C57BL/6NJ (RRID:IMSR_JAX:005304).

Suggested Control Mice
C57BL/6N

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.

Research Applications

  • Developmental Biology
  • Models for Human Disease
  • Reproduction
  • Research Tools

Strain Origin

Donor
Thomas Spencer, Ph.D., University of Missouri.
Primary Reference

Kelleher AM, Allen CC, Davis DJ, Spencer TE. Prss29 Cre recombinase mice are useful to study adult uterine gland function. Genesis. 2022 Dec;60(10-12):e23493. doi: 10.1002/dvg.23493. Epub 2022 Jul 22. (Medline PMID: 35866844)

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black
Eye
Black

Breeding

MMRRC Breeding System
Sib-mating
Generation
N?F?
Overall Breeding Performance
Good

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
7 to 9
Recommended wean age
3 Weeks
Average Pups Weaned
7 to 9

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.