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v6.5
A targeting vector (5'-arm-loxP-Malat1-FRT-Neo-FRT-loxP-3'-arm) was linearized and electroporated into the embryonic stem cell (ES) line V6.5 (SV129 × Bl6 F1 hybrid). G418-resistant ES clones were screened for correct homologous recombination at the Malat1 locus via colony PCR and Southern Blot analysis. Positive ES cell clones were injected into blastocysts derived from C57BL/6J mice (Charles River), and chimeric animals were set up for breeding with wildtype C57BL/6J mice to identify animals transmitting the correctly targeted Malat1 locus via the germ line. The heterozygous Malat1loxP,FRTNeo/+ mice generated from the ES cell clones were crossed with a transgenic CMV-Cre deleter line (in a C57Bl/6J genetic background) to remove the PGK-Neo selection cassette and the complete Malat1 gene, which was confirmed by PCR. As a result, nucleotides 5795370–5802920 from mouse chromosome 19qA(Assembly NCBI37/mm9) consisting of the complete 6982 nt Malat1 transcript sequence plus 251 nt upstream of the Malat1 transcription start site and 322 nt downstream of the Malat1 transcript end were removed. Mice were interbred. The genetic background appears to be primarily B6J, but an undetermined amount of 129 is likely to be present.
Eißmann M, Gutschner T, Hämmerle M, Günther S, Caudron-Herger M, Groß M, Schirmacher P, Rippe K, Braun T, Zörnig M, Diederichs S. Loss of the abundant nuclear non-coding RNA MALAT1 is compatible with life and development. RNA Biol. 2012 Aug;9(8):1076-87. doi: 10.4161/rna.21089. Epub 2012 Aug 1. (Medline PMID: 22858678)
Michalik KM, You X, Manavski Y, Doddaballapur A, Zörnig M, Braun T, John D, Ponomareva Y, Chen W, Uchida S, Boon RA, Dimmeler S. Long noncoding RNA MALAT1 regulates endothelial cell function and vessel growth. Circ Res. 2014 Apr 25;114(9):1389-97. doi: 10.1161/CIRCRESAHA.114.303265. Epub 2014 Mar 6. (Medline PMID: 24602777)
Cremer S, Michalik KM, Fischer A, Pfisterer L, Jaé N, Winter C, Boon RA, Muhly-Reinholz M, John D, Uchida S, Weber C, Poller W, Günther S, Braun T, Li DY, Maegdefessel L, Perisic Matic L, Hedin U, Soehnlein O, Zeiher A, Dimmeler S. Hematopoietic Deficiency of the Long Noncoding RNA MALAT1 Promotes Atherosclerosis and Plaque Inflammation. Circulation. 2019 Mar 5;139(10):1320-1334. doi: 10.1161/CIRCULATIONAHA.117.029015. Erratum in: Circulation. 2019 Jul 16;140(3):e161. (Medline PMID: 30586743)
Colony Surveillance Program and Current Health Reports
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.